Die alterierte Erythrocytenmembran

Kj, Halbhuber; Stibenz, D; Müller, Ulrich A.; Fröber, Rosemarie; Feuerstein, H; Meyer, H.; Augsten, Kurt; Linss, W

doi:10.1016/s0065-1281(82)80079-2

Cited by 13 publications

(2 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are only few reports, however, about sialic acid and phospholipid changes in banked human erythrocytes. Moore et al [14], Devenuto et al [4] and we [7,8] have found a distinct decrease of sialic acid as well as phospholip ids of erythrocyte membranes after 6 weeks of banking in ACD (or ACD-AG and CPD) medium. In contrast to these results, Pessina et al [15] report on a lack of significant sialic acid removal from erythrocytes after 32 days storage in ACD solution with and without adenine in this journal.…”

Section: Introductionmentioning

confidence: 75%

Metabolic Depletion of Erythrocytes is Accompanied by a Decrease of Sialic Acid during Blood Bank Storage: A Reply

Stibenz¹,

Feuerstein²,

Kj³

et al. 1982

Vox Sanguinis

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 75%

Metabolic Depletion of Erythrocytes is Accompanied by a Decrease of Sialic Acid during Blood Bank Storage: A Reply

Stibenz¹,

Feuerstein²,

Kj³

et al. 1982

Vox Sanguinis

View full text Add to dashboard Cite

show abstract

“…The "neo-antigen" is recognized by the antigen binding, Fab, region (3,10,11) of a specific IgG autoantibody in serum that attaches to it and initiates the removal of cells by macrophages (1)(2)(3)(4). A number of studies performed by us (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11) and by others have demonstrated the presence of IgG on senescent, damaged, and stored erythrocytes (12)(13)(14)(15)(16)(17)(18)(19)(20)1). In addition, workers in several laboratories have recently presented evidence that IgG binding is also involved in the removal of erythrocytes in diseases such as thalassemia (21) and sickle cell anemia (22,23).…”

mentioning

confidence: 99%

Oxidation as a possible mechanism of cellular aging: vitamin E deficiency causes premature aging and IgG binding to erythrocytes.

Kay¹,

Bosman²,

Shapiro³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

102

View full text Add to dashboard Cite

Senescent-cell antigen is a "neo-antigen" that appears on the surface of senescent cells and initiates IgG binding and cellular removal. As an approach to evaluating oxidation as a possible mechanism for generation of senescentcell antigen, we studied erythrocytes from vitamin E-deficient rats. Vitamin E is localized primarily in cellular membranes. Its major role is the termination of free-radical chain reactions propagated by the polyunsaturated fatty acids of membrane phospholipids. Results of our studies indicate that erythrocytes of all ages from vitamin E-deficient rats behave like old erythrocytes from normal rats, as determined by their susceptibility to phagocytosis, IgG binding, anion transport ability, and glyceraldehyde-3-phosphate dehydrogenase activity. Increased breakdown products of band 3 were observed with immunoblotting in membranes of erythrocytes from vitamin E-deficient rats. Breakdown products of band 3 are known to increase as cells age in normal individuals. The data suggest that oxidation may be a possible mechanism for erythrocyte aging and generation of senescent-cell antigen in vivo.

show abstract

Topo-optical investigations of the human erythrocyte glycocalyx-age related changes

Gliesing

Stibenz

et al. 1984

Histochemistry

View full text Add to dashboard Cite

The conformational state of the glycocalyx of the intact and altered erythrocyte membrane was studied by means of the topo-optical toluidine blue reaction, i.e. induced membrane birefringence. High membrane anisotropy represents the normal glycocalyx structure and its decline represents their perturbation. The results show that the glycocalyx structure is changed during ageing of the erythrocytes in vivo as well as in vitro. During fluid preservation, in vitro ageing and vesiculation of cells in vitro, a subpopulation of cells showed a decline of membrane anisotropy, but other cells demonstrated abnormally high values. In the latter cases, there is usually a correlation to spherocytes. From this point of view, it is to be assumed that spherogenesis during cell ageing is induced by cell vesiculation. This leads to a remodelling of an intact plasmalemma. In contrast, the cell fractions which are probably non-vesiculating seem to be more or less damaged by membrane and/or plasmic hydrolases. This can be mimicked by neuraminidase and protease treatment of erythrocytes in vitro. Membrane lesions caused by freeze preservation of red blood cells are rare. The topo-optical results are interpreted according to the assumptions of the theory of membrane anisotropy, i.e. the formation of dye-stuff micelles at distinct, clustered, sialylated carbohydrate chains of the glycophorin A.

show abstract

Die alterierte Erythrocytenmembran

Cited by 13 publications

References 86 publications

Metabolic Depletion of Erythrocytes is Accompanied by a Decrease of Sialic Acid during Blood Bank Storage: A Reply

Metabolic Depletion of Erythrocytes is Accompanied by a Decrease of Sialic Acid during Blood Bank Storage: A Reply

Oxidation as a possible mechanism of cellular aging: vitamin E deficiency causes premature aging and IgG binding to erythrocytes.

Topo-optical investigations of the human erythrocyte glycocalyx-age related changes

Contact Info

Product

Resources

About