DIDO3 Regulates Microtubule Stability at Transcriptional and Posttranslational Levels and is Needed for Fibroblast Adhesion and Movement

Pacios-Bras, Cristina; Pons, Tirso; Alonso-Guerrero, Astrid; Román‐García, Sara; Gutiérrez, Julio; Manrique, Pablo; Burgo, Fernando Gutiérrez del; Villares, Ricardo; Gallardo, Carmen Mora; Wely, Karel H. M. van; Carrasco, Yolanda R.; Martı́nez-A, Carlos

doi:10.2139/ssrn.3428482

Cited by 2 publications

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“…Although DIDO3, which is expressed ubiquitously in the organism, presumably has a more general function in determining chromatin structure, its profound influence on the B cell population led us to focus our study on this cell lineage. Speculations on the molecular mechanism involved have been treated elsewhere 31, 32,20,33 . Here we demonstrate that DIDO3 plays a role in HSC homing and differentiation, as well as in the later B cell differentiation process.…”

Section: Discussionmentioning

confidence: 99%

Chromatin reader Dido3 regulates the genetic network of B cell differentiation

Burgo

Pons

Luis

et al. 2021

Preprint

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The development of hematopoietic lineages is based on a complex balance of transcription factors whose expression depends on the epigenetic signatures that characterize each differentiation step. The B cell lineage arises from hematopoietic stem cells through the stepwise silencing of stemness genes and balanced expression of mutually regulated transcription factors, as well as DNA rearrangement. Here we report the impact on B cell differentiation of the lack of DIDO3, a reader of chromatin status, in the mouse hematopoietic compartment. We found reduced DNA accessibility in hematopoietic precursors, leading to severe deficiency in the generation of successive B cell differentiation stages. The expression of essential transcription factors and differentiation markers is affected, as is the somatic recombination process.

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Section: Discussionmentioning

confidence: 99%

Chromatin reader Dido3 regulates the genetic network of B cell differentiation

Burgo

Pons

Luis

et al. 2021

Preprint

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show abstract

“…Although DIDO3, which is expressed ubiquitously in the organism, presumably has a more general function in determining chromatin structure, its profound in uence on the B cell population led us to focus our study on this cell lineage. Speculations on the molecular mechanism involved have been treated elsewhere 31,32,20,33 . Here we demonstrate that DIDO3 plays a role in HSC homing and differentiation, as well as in the later B cell differentiation process.…”

Section: Discussionmentioning

confidence: 99%

Chromatin Reader Dido3 Regulates the Genetic Network of B Cell Differentiation

Burgo

Pons

Vázquez

et al. 2021

Preprint

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BACKGROUNDThe development of hematopoietic lineages is based on a complex balance of transcription factors whose expression depends on the epigenetic signatures that characterize each differentiation step. The B cell lineage arises from hematopoietic stem cells through the stepwise silencing of stemness genes and balanced expression of mutually regulated transcription factors, as well as DNA rearrangement, in a complex process involving epigenetic remodeling.RESULTSHere we report the impact on B cell differentiation of the lack of DIDO3, a reader of histone post-translational modifications, in the mouse hematopoietic compartment. We found reduced DNA accessibility in hematopoietic precursors, leading to a severe deficiency specifically in the generation of successive stages of B-cell differentiation. The expression of essential transcription factors and differentiation markers is impaired, as is the process of somatic recombination. DIDO3-deficient cells show transcriptional alterations of a number of polycomb repressive complex 2, suggesting the involvement of DIDO3 in determining the specific activity of PRC2 in the B cell lineage, including VH-DJH rearrangement. CONCLUSIONSTaken together, our data suggest that DIDO3 is an epigenetic reader involved in the specific differentiation of B cell precursors in the hematopoietic compartment of mice.

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DIDO3 Regulates Microtubule Stability at Transcriptional and Posttranslational Levels and is Needed for Fibroblast Adhesion and Movement

Cited by 2 publications

References 0 publications

Chromatin reader Dido3 regulates the genetic network of B cell differentiation

Chromatin reader Dido3 regulates the genetic network of B cell differentiation

Chromatin Reader Dido3 Regulates the Genetic Network of B Cell Differentiation

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