Diclofenac as a factor in the change of <i>Rhodococcus</i> metabolism

Tyumina, Elena A.; Bazhutin, G. A.; Vikhareva, E. V.; Selyaninov, A. A.; Ившина, И. Б.

doi:10.1088/1757-899x/487/1/012027

Cited by 6 publications

(5 citation statements)

References 11 publications

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“…Effective actinobacterial degradation of selected antibiotics, hormones, antiepileptics, analgesics, and nonsteroidal anti-inflammatory drugs (NSAIDs) has already been documented [12][13][14][15][16][17]. Earlier, we confirmed the ability of rhodococci to completely biodegrade pharmaceuticals from analgesics and antispasmodics, including paracetamol [18], drotaverine hydrochloride [19,20], and diclofenac sodium [21,22]. In this work, the analysis of possible Rhodococcus spp.…”

Section: Introductionsupporting

confidence: 67%

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

et al. 2021

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The article expands our knowledge on the variety of biodegraders of ibuprofen, one of the most frequently detected non-steroidal anti-inflammatory drugs in the environment. We studied the dynamics of ibuprofen decomposition and its relationship with the physiological status of bacteria and with additional carbon and energy sources. The involvement of cytoplasmic enzymes in ibuprofen biodegradation was confirmed. Within the tested actinobacteria, Rhodococcus cerastii IEGM 1278 was capable of complete oxidation of 100 μg/L and 100 mg/L of ibuprofen in 30 h and 144 h, respectively, in the presence of an alternative carbon source (n-hexadecane). Besides, the presence of ibuprofen induced a transition of rhodococci from single- to multicellular lifeforms, a shift to more negative zeta potential values, and a decrease in the membrane permeability. The initial steps of ibuprofen biotransformation by R. cerastii IEGM 1278 involved the formation of hydroxylated and decarboxylated derivatives with higher phytotoxicity than the parent compound (ibuprofen). The data obtained indicate potential threats of this pharmaceutical pollutant and its metabolites to biota and natural ecosystems.

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Section: Introductionsupporting

confidence: 67%

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

et al. 2021

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“…The cell size, shape, and SA/V are interconnected, and individual cells can change their size and shape to achieve an optimal SA/V (Harris and Theriot, 2018;Shi et al, 2021). Previously, we observed alterations in the SA/V ratio in rhodococci exposed to pharmaceuticals (Ivshina et al, 2019;Tyumina et al, 2019;Ivshina et al, , 2022b. For example, when exposed to toxic substrates such as DCF or naproxen, cells tend to reduce their SA/V to minimize the exposed cell surface for contact with the stressor (Ivshina et al, 2019(Ivshina et al, , 2022b.…”

Section: Morphometric Parameters Of Rhodococcus Cerastii Iegm 1243 Ce...mentioning

confidence: 95%

“…In addition to addressing the environmental impact of these NSAIDs, extensive research has elucidated the microbial biodegradation pathways of NSAIDs, as comprehensively reviewed by Guzik and Wojcieszyńska (2019) . However, despite significant efforts to examine the effects of NSAIDs on various organisms, our understanding of their specific impacts on microorganisms, particularly with respect to response mechanisms and adaptation patterns, remains limited ( Jiang et al, 2017 ; Ivshina et al, 2019 ; Tyumina et al, 2019 , 2020 ; Żur et al, 2020 ; Ivshina et al, 2021b ; Żur et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and metabolic adaptations of Rhodococcus cerastii strain IEGM 1243 to separate and combined effects of diclofenac and ibuprofen

Tyumina,

Bazhutin,

Kostrikina

et al. 2023

Front. Microbiol.

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IntroductionThe increasing use of non-steroidal anti-inflammatory drugs (NSAIDs) has raised concerns regarding their environmental impact. To address this, understanding the effects of NSAIDs on bacteria is crucial for bioremediation efforts in pharmaceutical-contaminated environments. The primary challenge in breaking down persistent compounds lies not in the biochemical pathways but in capacity of bacteria to surmount stressors.MethodsIn this study, we examined the biodegradative activity, morphological and physiological changes, and ultrastructural adaptations of Rhodococcus cerastii strain IEGM 1243 when exposed to ibuprofen, diclofenac, and their mixture.Results and DiscussionOur findings revealed that R. cerastii IEGM 1243 exhibited moderate biodegradative activity towards the tested NSAIDs. Cellular respiration assay showed higher metabolic activity in the presence of NSAIDs, indicating their influence on bacterial metabolism. Furthermore, catalase activity in R. cerastii IEGM 1243 exposed to NSAIDs showed an initial decrease followed by fluctuations, with the most significant changes observed in the presence of DCF and the NSAID mixture, likely influenced by bacterial growth phases, active NSAID degradation, and the formation of multicellular aggregates, suggesting potential intercellular synergy and task distribution within the bacterial community. Morphometric analysis demonstrated alterations in size, shape, and surface roughness of cells exposed to NSAIDs, with a decrease in surface area and volume, and an increase in surface area-to-volume ratio (SA/V). Moreover, for the first time, transmission electron microscopy confirmed the presence of lipid inclusions, polyphosphates, and intracellular membrane-like structures in the ibuprofen-treated cells.ConclusionThese results provide valuable insights into the adaptive responses of R. cerastii IEGM 1243 to NSAIDs, shedding light on the possible interaction between bacteria and pharmaceutical compounds in the environment.

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“…Within Actinobacteria phylum (Corynebacteriaceae order), Rhodococcus represents one of the most extraordinary genera in terms of catabolic versatility, since the members of this genus deploy an extensive asset of genes encoding enzymes enabling the biotransformation and/or biodegradation of a wide array of organic compounds, contaminants, pharmaceuticals, and wastes [1][2][3][4][5][6][7][8][9]. The remarkable metabolic capabilities of Rhodococcus species together with their strong persistence under difficult conditions are due to their peculiar cell features including the cell envelope (containing mycolic acids, and peptidoglycan), hydrophobicity, a specific phospholipid profile, and large and plastic genomes with a multiplicity of genes for catabolic and anabolic processes, and stress response [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Genome-Based Exploration of Rhodococcus Species for Plastic-Degrading Genetic Determinants Using Bioinformatic Analysis

et al. 2022

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Plastic polymer waste management is an increasingly prevalent issue. In this paper, Rhodococcus genomes were explored to predict new plastic-degrading enzymes based on recently discovered biodegrading enzymes for diverse plastic polymers. Bioinformatics prediction analyses were conducted using 124 gene products deriving from diverse microorganisms retrieved from databases, literature data, omic-approaches, and functional analyses. The whole results showed the plastic-degrading potential of Rhodococcus genus. Among the species with high plastic-degrading potential, R. erythropolis, R. equi, R. opacus, R. qingshengii, R. fascians, and R. rhodochrous appeared to be the most promising for possible plastic removal. A high number of genetic determinants related to polyester biodegradation were obtained from different Rhodococcus species. However, score calculation demonstrated that Rhodococcus species (especially R. pyridinivorans, R. qingshengii, and R. hoagii) likely possess PE-degrading enzymes. The results identified diverse oxidative systems, including multicopper oxidases, alkane monooxygenases, cytochrome P450 hydroxylases, para-nitrobenzylesterase, and carboxylesterase, and they could be promising reference sequences for the biodegradation of plastics with C−C backbone, plastics with heteroatoms in the main chain, and polyesters, respectively. Notably, the results of this study could be further exploited for biotechnological applications in biodegradative processes using diverse Rhodococcus strains and through catalytic reactions.

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Diclofenac as a factor in the change of Rhodococcus metabolism

Cited by 6 publications

References 11 publications

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

Response of Rhodococcus cerastii IEGM 1278 to toxic effects of ibuprofen

Phenotypic and metabolic adaptations of Rhodococcus cerastii strain IEGM 1243 to separate and combined effects of diclofenac and ibuprofen

Genome-Based Exploration of Rhodococcus Species for Plastic-Degrading Genetic Determinants Using Bioinformatic Analysis

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