Diclofenac and atrazine restrict the growth of a synchronous Chlamydomonas reinhardtii population via various mechanisms

Harshkova, Darya; Majewska, Maria Dorota; Pokora, Wojciech; Baścik-Remisiewicz, Agnieszka; Tułodziecki, Szymon; Aksmann, Anna

doi:10.1016/j.aquatox.2020.105698

Cited by 18 publications

(10 citation statements)

References 70 publications

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“…The use of synchronous cell cultures allowed us to verify the hypothesis that one of the causes of DCF phytotoxicity was the disorder of cell cycle progression, and extend the findings of our previous works [9,35,36]. The anti-proliferative effect of DCF, reported in the literature for mammalian cells [47], also appeared to be valid for C. reinhardtii cells.…”

Section: Discussionsupporting

confidence: 74%

“…All the concentrations of DCF used in our study were higher than the environmentally relevant concentrations ranging from 1 ng/L to 1 mg/L [37][38][39]. Yet, the cultures of C. reinhardtii were able to cope with all the concentrations except the highest one, DCF75, for at least 24 h. In our previous study, the asynchronous cells were able to cope with even higher DCF concentrations for 24 h [36]. In our experiments, DCF75 caused a strong cytotoxic effect, lysis, in which about 70% of cells died during the first hours of the cell cycle.…”

Section: Discussionmentioning

confidence: 45%

“…Data described in our previous studies showed that in asynchronous cultures of C. reinhardtii, DCF caused oxidative stress and affected photosynthesis [9,35,36]. However, it was not clear what was its effect would be on growth and cell reproduction (cell cycle) and if they were both affected similarly or not.…”

Section: Discussionmentioning

confidence: 92%

“…Interestingly, similar immediate DCF toxic effects were observed within 1 h of the DCF50 culture illumination, which resulted in about a 30% drop in cell numbers (Figure 4). It may be assumed that some daughter cells that consumed their energy storage reserves during the dark phase were unable to cope with the combination of light stress combined with the impact of DCF50, possibly due to its toxic effect on young chloroplasts and oxidative stress generation [9,36]. The above assumption is supported by the observation that immediately after th dark/light transition, the overall efficiency of photosynthesis (performance index, quantum yield) transiently dropped in control cells, and this effect was enhanced by DCF50 (Figure 3).…”

Section: Discussionmentioning

confidence: 99%

“…As the above-mentioned observations confirm that DCF alters the cell cycle progression rather than single cell growth [36], we focused on cell cycle-related parameters. As mentioned above, DCF10 and DCF25 concentrations did not affect individual growth of C. reinhardtii cells (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

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Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii

Harshkova

Liakh

Bialevich

et al. 2021

Cells

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The aim of the study was to verify the hypothesis that a potential cause of the phytotoxicity of diclofenac (DCF, a non-steroidal anti-inflammatory drug) is an effect of cell cycle progression. This research was conducted using synchronous cultures of a model organism, green alga Chlamydomonas reinhardtii. The project examined DCF effects on selected parameters that characterize cell cycle progression, such as cell size, attainment of commitment points, DNA replication, number of nuclei formed during cells division and morphology of cells in consecutive stages of the cell cycle, together with the physiological and biochemical parameters of algae cells at different stages. We demonstrated that individual cell growth remained unaffected, whereas cell division was delayed in the DCF-treated groups grown in continuous light conditions, and the number of daughter cells from a single cell decreased. Thus, the cell cycle progression is a target affected by DCF, which has a similar anti-proliferative effect on mammalian cells.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 45%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii

Harshkova

Liakh

Bialevich

et al. 2021

Cells

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Differential Effects of Atrazine on Chlorophyceae Species and Association with Morphology, Photosynthesis, Chlorophyll Content, and Glutathione‐S‐Transferase Activity

Castro

Silva

Machado

et al. 2022

Enviro Toxic and Chemistry

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Atrazine is a herbicide widely used in the control of weeds in crops such as corn, sugar cane, and sorghum. It is often found in aquatic environments, where it can potentially endanger nontarget organisms such as microalgae. The present study evaluated atrazine toxicity to seven different species of Chlorophyceae and the tolerance of the species to the herbicide was related to morphological, photosynthetic, chlorophyll‐a content and the activity of the glutathione‐S‐transferase enzyme (GST). The comparison of median effect concentration (EC50) values for growth inhibition indicates higher toxicity of atrazine for Pseudopediastrum boryanum and Desmodesmus communis, intermediate toxicity for Ankistrodesmus densus, Chlamydomonas puliminiorfes, and Raphidocelis subcapitata, and lower toxicity for Kirchneriella lunaris and Ankistrodesmus falcatus (EC50: 38, 42, 66, 103, 248, 1004, and 1585 µg L−1 atrazine, respectively). Principal component analysis (PCA) with algal characteristics suggested that the atrazine‐sensitive algae P. boryanum and D. communis were positively associated with photosynthetic levels and negatively associated with GST activity and chlorophyll‐a concentration. The PCA also suggested that the atrazine‐tolerant algae A. falcatus and K. lunaris were positively associated with morphological parameters, where the larger the cell size, the more tolerant. Although it is difficult to associate a single characteristic of algae as the key factor determining the tolerance to atrazine, results presented in this work indicate that the cell area, the photosynthetic parameters (mainly saturating irradiance), chlorophyll‐a content, and the biotransformation by GST in combination may be potential predictors for the differential tolerance of Chlorophyceae species to the herbicide. Environ Toxicol Chem 2022;41:1675–1685. © 2022 SETAC

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Superoxide dismutase in Arabidopsis and Chlamydomonas: diversity, localization, regulation, and role

Shams,

Pokora,

Khadivi

et al. 2024

Plant Soil

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Diclofenac and atrazine restrict the growth of a synchronous Chlamydomonas reinhardtii population via various mechanisms

Cited by 18 publications

References 70 publications

Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii

Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii

Differential Effects of Atrazine on Chlorophyceae Species and Association with Morphology, Photosynthesis, Chlorophyll Content, and Glutathione‐S‐Transferase Activity

Superoxide dismutase in Arabidopsis and Chlamydomonas: diversity, localization, regulation, and role

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