Diclidophora luscae (Monogenea: Diclidophoridae) in pouting, Trisopterus luscus (Linnaeus, 1758) from the northeast Atlantic; epidemiology, morphology, molecular and phylogenetic analysis

Abstract

Diclidophora (Monogenea) species are gill parasites with a stenoxenic specificity occurring only in Gadiformes. Epidemiological, morphological, molecular and phylogenetic studies were performed on 594 Diclidophora specimens collected from 213 Trisopterus luscus captured in the northeast Atlantic off the Portuguese coast during 2012, 2013 and 2020. Prevalence, parasite abundance and infection intensity were determined. Positive correlation between fish weight and length and infection intensity was observed. The effects of preservation on the parasite morphological features were studied, highlighting that specimen's identification should be reinforced by molecular studies. A sequence of D. luscae capelanii from T. capelanus captured in the Mediterranean Sea included in the 28S rDNA molecular analysis was nested within a robust D. luscae clade. Data analysis suggested that this species is in fact D. luscae, which is compatible with T. luscus and T. capelanus. The identity of fish hosts was confirmed by barcoding. For the first time, data on the infection parameters is shown, highlighting the importance of including this parasite in the monitoring plans for a holistic approach with possible effects for the management of pouting resources aiming of attaining sustainable development and biodiversity conservation measures, according to the 14th objective of the 2030 agenda.

Morphological and genetic identification of the gill monogenean parasite (Diclidophora merlangi) that infects Twobar Seabream Fish (Acanthopagrus bifasciatus) in the Arabian Gulf, Saudi Arabia

“…Molecular findings validated the distinction of the organism’s identity as D. merlangi , which differs from D. luscae , D. minor and D. denticulata . Ramos et al . (2022) studied D. luscae from Trisopterus luscus and validated its molecular identification, grouping it alongside D. minor and D. denticulata into a single clade.…”

“…(2022) studied D. luscae from Trisopterus luscus and validated its molecular identification, grouping it alongside D. minor and D. denticulata into a single clade. Ramos et al . (2022) sequences were determined to be more than 99 % identical to those described before ( Mollaret et al ., 2000 ; Jovelin & Justine, 2001 ), who classified it as D. luscae capelanii .…”

“…The organism ( D. luscae capelanii ) was recovered from T. capelanus in the Mediterranean Sea and after the host harboring it ( T. luscus ) from the Atlantic Ocean ( Jovelin & Justine, 2001 ). Ramos et al . (2022) recovered the same organism from T. lusca , hence, the International Code of Zoological Nomenclature (ICZN) used the name D. luscae .…”