Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation

Coll, Olga; Guitart, Tanit; Villalba, Ana; Papin, Catherine; Simonelig, Martine; Gebauer, Fátima

doi:10.1261/rna.065417.117

Cited by 12 publications

(14 citation statements)

References 51 publications

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“…Thus, it is likely that Q17D57 has similar function to Tailor, which could be a topic for further studies. Alternatively, in D. melanogaster polyadenylation of Toll and R2D2 mRNA happens via Dcr2-dependent mechanism, as Dcr2 interacts with cytoplasmic poly(A) polymerase Wispy [ 58 ]. Considering that Q17D57 has a conserved nucleotidyltransferase domain of poly(A) polymerases, it might be that Ae.…”

Section: Discussionmentioning

confidence: 99%

aBravo Is a Novel Aedes aegypti Antiviral Protein That Interacts with, but Acts Independently of, the Exogenous siRNA Pathway Effector Dicer 2

Varjak

Gestuveo

Burchmore

et al. 2020

Viruses

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Mosquitoes, such as Aedes aegypti, can transmit arboviruses to humans. The exogenous short interfering RNA (exo-siRNA) pathway plays a major antiviral role in controlling virus infection in mosquito cells. The Dicer 2 (Dcr2) nuclease is a key effector protein in this pathway, which cleaves viral double-stranded RNA into virus-derived siRNAs that are further loaded onto an effector called Argonaute 2 (Ago2), which as part of the multiprotein RNA-induced silencing complex (RISC) targets and cleaves viral RNA. In order to better understand the effector protein Dcr2, proteomics experiments were conducted to identify interacting cellular partners. We identified several known interacting partners including Ago2, as well as two novel and previously uncharacterized Ae. aegypti proteins. The role of these two proteins was further investigated, and their interactions with Dcr2 verified by co-immunoprecipitation. Interestingly, despite their ability to interact with Ago2 and Piwi4, neither of these proteins was found to affect exo-siRNA silencing in a reporter assay. However, one of these proteins, Q0IFK9, subsequently called aBravo (aedine broadly active antiviral protein), was found to mediate antiviral activity against positive strand RNA arboviruses. Intriguingly the presence of Dcr2 was not necessary for this effect, suggesting that this interacting antiviral effector may act as part of protein complexes with potentially separate antiviral activities.

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Section: Discussionmentioning

confidence: 99%

aBravo Is a Novel Aedes aegypti Antiviral Protein That Interacts with, but Acts Independently of, the Exogenous siRNA Pathway Effector Dicer 2

Varjak

Gestuveo

Burchmore

et al. 2020

Viruses

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“…Recently, Dcr2 has been shown to be necessary for this process. Dcr2 interacts with Wispy, the cytoplasmic poly‐A polymerase and promotes cytoplasmic polyadenylation of Toll mRNA and r2d2 mRNA .…”

Section: Dicer In Transcriptional Regulation and Rna Processingmentioning

confidence: 99%

Noncanonical functions of microRNA pathway enzymes – Drosha, DGCR8, Dicer and Ago proteins

2018

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MicroRNAs (miRNAs) are small regulatory noncoding RNAs that are generated in the canonical RNA interference (RNAi) pathway. Drosha, DiGeorge syndrome critical region 8 (DGCR8) and Dicer are key players in miRNA biogenesis. Argonaute (Ago) proteins bind to miRNAs and are guided by them to find messenger RNA targets and carry out post-transcriptional silencing of protein-coding genes. Recently, emerging evidence suggests that RNAi factors have a range of noncanonical functions that are beyond miRNA biogenesis. These functions pertain to various biological processes, such as development, transcriptional regulation, RNA processing and maintenance of genome integrity. Here, we review recent literature reporting miRNA-independent, noncanonical functions of Drosha, DGCR8, Dicer and Ago proteins and discuss the importance of these functions.

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“…Of note, at least two immunity related genes, Toll and R2D2, depend on Dcr-2 for their poly-adenylation (Coll et al, 2018). This study suggests that a complex composed of Dcr-2 and Wispy, a cytoplasmic poly(A) polymerase, is necessary for cytoplasmic polyadenylation of these genes.…”

Section: Regulation Of Gene Expression By Dcr-1 and Dcr-2 Contributes To Virushost Interaction In Fliesmentioning

confidence: 75%

Sensing and signalling viral infection in drosophila

Schneider¹,

Imler²

2021

Developmental & Comparative Immunology

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The fruitfly Drosophila melanogaster is a valuable model to unravel mechanisms of innate immunity, in particular in the context of viral infections. RNA interference, and more specifically the small interfering RNA pathway, is a major component of antiviral immunity in drosophila. In addition, the contribution of inducible transcriptional responses to the control of viruses in drosophila and other invertebrates is increasingly recognized. In particular, the recent discovery of a STING-IKK -Relish signalling cassette in drosophila has confirmed that NF-B transcription factors play an important role in the control of viral infections, in addition to bacterial and fungal infections. Here, we review recent developments in the field, which begin to shed light on the mechanisms involved in sensing of viral infections and in signalling leading to production of antiviral effectors.

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Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation

Cited by 12 publications

References 51 publications

aBravo Is a Novel Aedes aegypti Antiviral Protein That Interacts with, but Acts Independently of, the Exogenous siRNA Pathway Effector Dicer 2

aBravo Is a Novel Aedes aegypti Antiviral Protein That Interacts with, but Acts Independently of, the Exogenous siRNA Pathway Effector Dicer 2

Noncanonical functions of microRNA pathway enzymes – Drosha, DGCR8, Dicer and Ago proteins

Sensing and signalling viral infection in drosophila

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