Dicalcin, a zona pellucida protein that regulates fertilization competence of the egg coat in Xenopus laevis

Abstract

Fertilization is a highly coordinated process whereby sperm interact with the egg-coating envelope (called the zona pellucida, ZP) in a taxon-restricted manner, Fertilization triggers the resumption of the cell cycle of the egg, ultimately leading to generation of a new organism that contains hereditary information of the parents. The complete sperm-ZP interaction comprises sperm recognition of the ZP, the acrosome reaction, penetration of the ZP, and fusion with the egg. Recent evidence suggests that these processes involve oligosaccharides associated with a ZP constituent (termed ZP protein), the polypeptide backbone of a ZP protein, and/or the proper three-dimensional filamentous structure of the ZP. However, a detailed description of the molecular mechanisms involved in sperm-ZP interaction remains elusive. Recently, I found that dicalcin, a novel ZP protein-associated protein, suppresses fertilization through its association with gp41, the frog counterpart of the mammalian ZPC protein. This review focuses on molecular aspects of sperm-ZP interaction and describes the fertilization-suppressive function of dicalcin and associated molecular mechanisms. The amount of dicalcin in the ZP significantly correlates with alteration of the lectin-staining pattern within the ZP and the orientation pattern of ZP filaments, which may assist in elucidating the complex molecular mechanisms that underlie sperm-ZP interaction.

The C-terminal region of OVGP1 remodels the zona pellucida and modifies fertility parameters

“…In Xenopus laevis , the fertilization competence of the egg-coat envelope is regulated by direct interaction between dicalcin and gp41, a frog orthologue of mammalian ZP component ZP339. Dicalcin might act as a key regulatory protein involved in mediating fertilization competence by modifying the three dimensional structure of egg coat ZP filaments40. Likewise, OVGP1 might generate a structural microenvironment necessary for sperm to reach the egg plasma membrane.…”