Dibutyl phthalate degradation by Enterobacter sp. T5 isolated from municipal solid waste in landfill bioreactor

Fang, Chengran; Yao, Jun; Zheng, Yuan-Ge; Jiang, Chenjing; Hu, Lifang; Wu, Yu-Yong; Shen, Dongsheng

doi:10.1016/j.ibiod.2010.04.010

Cited by 99 publications

(38 citation statements)

References 22 publications

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“…The 16S rRNA gene was amplified from genomic DNA using universal bacterial primers F27 (5 0 -AGA GTT TGA TCC TGG CTC AG-3 0 ) and R1492 (5 0 -GGT TAC CTT GTT ACG ACT T-3 0 ). The PCR amplification procedure was as previously described by Fang et al (2010). The PCR products were visualized by agarose gel electrophoresis and purified using an EZ-10 Spin Column DNA Gel Extraction Kit (Bio Basic, Inc.).…”

Section: Microbial Identification and Phylogenetic Analysismentioning

confidence: 99%

Isolation and characterization of four di-n-butyl phthalate (DBP)-degrading Gordonia sp. strains and cloning the 3,4-phthalate dioxygenase gene

Wang

Dai

et al. 2011

World J Microbiol Biotechnol

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Four aerobic bacterial strains capable of utilizing di-n-butyl phthalate (DBP) as the sole source of carbon and energy were isolated from river sediments. Based on the morphology, biochemical characterization, and 16S rRNA gene sequence analysis, they were identified as Gordonia sp. The optimal conditions for DBP degradation by these strains were found to be pH 7.0, 30°C, and stirring at 175 rpm. These four strains could degrade, respectively, 96, 98, 98, and 78% of DBP (400 mg l -1 ) as well as dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-octyl phthalate (DOP), di-isooctyl phthalate (DIOP), and di-isononyl phthalate (DINP). Furthermore, partial sequences of the gene for 3,4-phthalate dioxygenase were obtained from all four strains. To our knowledge, this is the first time that the 3,4-phthalate dioxygenase gene has been successfully cloned from Gordonia sp.

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Section: Microbial Identification and Phylogenetic Analysismentioning

confidence: 99%

Isolation and characterization of four di-n-butyl phthalate (DBP)-degrading Gordonia sp. strains and cloning the 3,4-phthalate dioxygenase gene

Wang

Dai

et al. 2011

World J Microbiol Biotechnol

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“…Bacterial species belonging to Stenotrophomonas were previously found to be capable of using either nonylphenol or octylphenol as a sole carbon source. 44 For species belonging to the well-known human-pathogenic and plant association Enterobacter, degradation of EDCs has been reported particularly for bisphenol A 45 , polychlorinated biphenyls 46 , endosulfan 47 , dibutyl phthalate 48 and nonylphenol 49 .…”

Section: Degradation Of Nonylphenol By Bacterial Speciesmentioning

confidence: 99%

Isolation and characterisation of endocrine disruptor nonylphenol-using bacteria from South Africa

Qhanya¹,

Mthakathi²,

Boucher³

et al. 2017

S. Afr. J. Sci.

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Endocrine disrupting chemicals (EDCs) are synthetic chemicals that alter the function of endocrine systems in animals including humans. EDCs are considered priority pollutants and worldwide research is ongoing to develop bioremediation strategies to remove EDCs from the environment. An understanding of indigenous microorganisms is important to design efficient bioremediation strategies. However, much of the information available on EDCs has been generated from developed regions. Recent studies have revealed the presence of different EDCs in South African natural resources, but, to date, studies analysing the capabilities of microorganisms to utilise/degrade EDCs have not been reported from South Africa. Here, we report for the first time on the isolation and enrichment of six bacterial strains from six different soil samples collected from the Mpumalanga Province, which are capable of utilising EDC nonylphenol as a carbon source. Furthermore, we performed a preliminary characterisation of isolates concerning their phylogenetic identification and capabilities to degrade nonylphenol. Phylogenetic analysis using 16S rRNA gene sequencing revealed that four isolates belonged to Pseudomonas and the remaining two belonged to Enterobacteria and Stenotrophomonas. All six bacterial species showed degradation of nonylphenol in broth cultures, as HPLC analysis revealed 41–46% degradation of nonylphenol 12 h after addition. The results of this study represent the beginning of identification of microorganisms capable of degrading nonylphenol, and pave the way for further exploration of EDC-degrading microorganisms from South Africa.

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“…Phthalate acid esters (PAEs) are a class of organic compounds widely used as plasticizers to improve the properties of plastics (He et al, 2015;Fang et al, 2010). Surveys showed worldwide production of PAEs is approximately 6 million tons per year and a large amount of these compounds are released into the air, sediment, natural water, wastewater, and soils (Bauer and Herrmann, 1997;Mackintosh et al, 2006;Shailaja et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of aging process on adsorption of diethyl phthalate in soils amended with bamboo biochar

et al. 2016

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Dibutyl phthalate degradation by Enterobacter sp. T5 isolated from municipal solid waste in landfill bioreactor

Cited by 99 publications

References 22 publications

Isolation and characterization of four di-n-butyl phthalate (DBP)-degrading Gordonia sp. strains and cloning the 3,4-phthalate dioxygenase gene

Isolation and characterization of four di-n-butyl phthalate (DBP)-degrading Gordonia sp. strains and cloning the 3,4-phthalate dioxygenase gene

Isolation and characterisation of endocrine disruptor nonylphenol-using bacteria from South Africa

Effect of aging process on adsorption of diethyl phthalate in soils amended with bamboo biochar

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