Diazepam and Cl- transport in rat parotid acinar cells.

ABSTRACT-To obtain more insight into the physiological role of gamma-aminobutyric acid (GABA) in rat salivary glands, we measured the concentration of GABA and the activities of its biosynthetic and metabolic enzymes, glutamate decarboxylase (GAD) and GABA transaminase (GABA-T). The GABA concentrations in rat parotid and submandibular glands were 10.0 and 14.3 nmol/g weight, respectively, which were 0.6-0.8% of the levels in the brain (cerebellum and medulla oblongata), whereas glutamic acid (Glu) was abundant in the two glands. These GABA levels in the two glands were significantly decreased by administration of semicarbazide (200 mg/kg, i.p.), a GAD inhibitor, and increased by gabaculine (50 mg/kg, i.p.), a GABA-T inhibitor. The activities of both GAD and GABA-T were also detected in homogenates of the two salivary glands, but they were lower than those in the brain. However, kinetic analysis showed that the values of Michaelis constants for Glu and GABA in both enzyme reactions in these two glands were similar to those in the brain. These results indicate that GABA and its biosynthetic and metabolic enzymes are present in rat salivary glands as well as the brain.

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Existence of Gamma-Aminobutyric Acid and Its Biosynthetic and Metabolic Enzymes in Rat Salivary Glands

Sawaki

Ouchi

Sato

et al. 1995

Japanese Journal of Pharmacology

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Role of Metallothionein in Protection against Renal Oxidative Stress Induced by cis-Diamminedichloroplatinum (II) in Glutathione-Depleted Mice.

Nakagawa

Satoh

Naganuma

et al. 1996

Tohoku J. Exp. Med.

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Diazepam and Cl- transport in rat parotid acinar cells.

Cited by 2 publications

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Existence of Gamma-Aminobutyric Acid and Its Biosynthetic and Metabolic Enzymes in Rat Salivary Glands

Existence of Gamma-Aminobutyric Acid and Its Biosynthetic and Metabolic Enzymes in Rat Salivary Glands

Role of Metallothionein in Protection against Renal Oxidative Stress Induced by cis-Diamminedichloroplatinum (II) in Glutathione-Depleted Mice.

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