Diastereomeric differentiation of two pairs of glycal derivatives by electrospray ionization tandem mass spectrometry

Abstract

Scheme 4. Proposed cross-ring cleavage mechanisms for (a) isomer 3 and (b) isomer 4.

Rapid differentiation of nucleotide phosphoramidate diastereomers by electrospray ionization tandem mass spectrometry

“…Although mass spectrometry traditionally has not been widely recognized as a technique for characterizing stereoisomers, numerous reports have demonstrated the application of mass spectrometry to differentiate diastereomers based on the stereospecific fragmentation behavior of diastereomeric compounds. [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] However, there has not been a systematic study on nucleotide phosphoramidate diastereomers using mass spectrometry.…”

“…The challenge for the characterization of stereoisomers by mass spectrometry is that isomeric molecules have the identical mass‐to‐charge ratio, and therefore, they cannot be distinguished. Although mass spectrometry traditionally has not been widely recognized as a technique for characterizing stereoisomers, numerous reports have demonstrated the application of mass spectrometry to differentiate diastereomers based on the stereospecific fragmentation behavior of diastereomeric compounds . However, there has not been a systematic study on nucleotide phosphoramidate diastereomers using mass spectrometry.…”