Diarylheptanoids from Curcuma phaeocaulis Suppress IL-6-Induced STAT3 Activation

Jang, Hyun‐Jae; Park, Eun Jae; Lee, Seung-Jae; Lim, Hyung Jin; Jo, Jin Ha; Lee, Seung; Rho, Mun‐Chual

doi:10.1055/a-0668-0962

Cited by 9 publications

(7 citation statements)

References 46 publications

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“…It has been demonstrated that the Stat3 pathway is implicated in the development of cancer . We next measured the effect of icaritin on the Stat3 pathway.…”

Section: Resultsmentioning

confidence: 99%

“…It has been demonstrated that the Stat3 pathway is implicated in the development of cancer. 15 We next measured the effect of icaritin on the Stat3 pathway. The decreased expressions of p-Stat3 and Bmi-1 were found in both U87 and T98G cells when icaritin (10 or 20 μM) was added to the cultures ( Figure 3A and 3B).…”

Section: Icaritin Suppressed the Stat3 Pathway In Of Gbm Cellsmentioning

confidence: 99%

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Icaritin inhibits glioblastoma cell viability and glycolysis by blocking the IL‐6/Stat3 pathway

Liang

Wang

2018

J of Cellular Biochemistry

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Glioblastoma (GBM) is a common and aggressive brain tumor that is associated with significant increase in glycolysis for energy production. Icaritin is a natural compound and exhibits anticancer activity in GBM. However, the effect of icaritin on glycolysis in GBM cells remains unclear. The aim of the current study was to investigate the effect of icaritin on glycolysis in GBM cells. The human GBM cell lines U87 and T98G were treated with icaritin or the inhibitor of Stat3 (S3I‐201) in the presence or absence of recombinant human interleukin (IL)‐6. Cell viability was measured using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5 diphenyltetrazolium bromide (MTT) assay. The glycolysis was analyzed by detecting the glucose consumption and lactate production. The Western blot analysis was conducted to detect the expressions of hexokinase 2 (HK2), signal transducer and activator of transcription 3 (Stat3), p‐Stat3, and B lymphoma Mo‐MLV insertion region 1 (Bmi‐1). Results showed that icaritin inhibited the viability of U87 and T98G cells in a dose‐dependent manner. The decreased glucose consumption and lactate production, accompanied by reduced expressions of HK2, were found in both U87 and T98G cells. Icaritin inhibited the IL‐6/Stat3 pathway, which is evidenced by the decreased expressions of p‐Stat3 and Bmi‐1. IL‐6 treatment induced the phosphorylation of Stat3 and Bmi‐1 expression, increased cell viability, as well as elevated glucose consumption, lactate production, and HK2 expression; however, the effects of IL‐6 were attenuated by icaritin or S3I‐201 treatment. In conclusion, icaritin exerted inhibitory effects on cell viability and glycolysis in GBM cells, which was mediated by the IL‐6/Stat3 pathway.

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“…It has been demonstrated that the Stat3 pathway is implicated in the development of cancer . We next measured the effect of icaritin on the Stat3 pathway.…”

Section: Resultsmentioning

confidence: 99%

Section: Icaritin Suppressed the Stat3 Pathway In Of Gbm Cellsmentioning

confidence: 99%

Icaritin inhibits glioblastoma cell viability and glycolysis by blocking the IL‐6/Stat3 pathway

Liang

Wang

2018

J of Cellular Biochemistry

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“…Interestingly, a recent study has reported that curcumin can exhibit significant inhibitory activity on IL-6-induced STAT3 activation with an IC 50 value FIGURE 3 | Snapshot of the browse page. of 1.6 µM, indicating its potential to be a candidate for the treatment of cancer related to the IL-6/STAT3 signaling pathway (Jang et al, 2019). The rest of the predicted targets provide novel potential proteins involved in cancer immunity by curcumin, which deserve to be further validated by experimental assays.…”

Section: Curcuminmentioning

confidence: 99%

TCMIO: A Comprehensive Database of Traditional Chinese Medicine on Immuno-Oncology

Liu

Cai

Du³

et al. 2020

Front. Pharmacol.

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Advances in immuno-oncology (IO) are making immunotherapy a powerful tool for cancer treatment. With the discovery of an increasing number of IO targets, many herbs or ingredients from traditional Chinese medicine (TCM) have shown immunomodulatory function and antitumor effects via targeting the immune system. However, knowledge of underlying mechanisms is limited due to the complexity of TCM, which has multiple ingredients acting on multiple targets. To address this issue, we present TCMIO, a comprehensive database of Traditional Chinese Medicine on Immuno-Oncology, which can be used to explore the molecular mechanisms of TCM in modulating the cancer immune microenvironment. Over 120,000 small molecules against 400 IO targets were extracted from public databases and the literature. These ligands were further mapped to the chemical ingredients of TCM to identify herbs that interact with the IO targets. Furthermore, we applied a network inference-based approach to identify the potential IO targets of natural products in TCM. All of these data, along with cheminformatics and bioinformatics tools, were integrated into the publicly accessible database. Chemical structure mining tools are provided to explore the chemical ingredients and ligands against IO targets. Herb-ingredient-target networks can be generated online, and pathway enrichment analysis for TCM or prescription is available. This database is functional for chemical ingredient structure mining and network analysis for TCM. We believe that this database provides a comprehensive resource for further research on the exploration of the mechanisms of TCM in cancer immunity and TCM-inspired identification of novel drug leads for cancer immunotherapy. TCMIO can be publicly accessed at http:// tcmio.xielab.net.

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“…S16). IC 50 values were calculated by nonlinear regression analysis as described previously [35]. RAW264.7 cell cytotoxicity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay [34].…”

Section: Isolation Of Compounds 1 and 3 Pulverized Stem Ofmentioning

confidence: 99%

Anti-Inflammatory Activity of Diterpenoids fromCelastrus orbiculatusin Lipopolysaccharide-Stimulated RAW264.7 Cells

Jang

Kim

Park

et al. 2020

Journal of Immunology Research

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Celastrus orbiculatus Thunb has been known as an ethnopharmacological medicinal plant for antitumor, anti-inflammatory, and analgesic effects. Although various pharmacological studies of C. orbiculatus extract has been reported, an anti-inflammatory mechanism study of their phytochemical constituents has not been fully elucidated. In this study, compounds 1–17, including undescribed podocarpane-type trinorditerpenoid (3), were purified from C. orbiculatus and their chemical structure were determined by high-resolution electrospray ionization mass (HRESIMS) and nuclear magnetic resonance (NMR) spectroscopic data. To investigate the anti-inflammatory activity of compounds 1–17, nitric oxide (NO) secretion was evaluated in LPS-treated murine macrophages, RAW264.7 cells. Among compounds 1–17, deoxynimbidiol (1) and new trinorditerpenoid (3) showed the most potent inhibitory effects (IC50: 4.9 and 12.6 μM, respectively) on lipopolysaccharide- (LPS-) stimulated NO releases as well as proinflammatory mediators, such as inducible nitric oxide (iNOS), cyclooxygenase- (COX-) 2, interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α. Its inhibitory activity of proinflammatory mediators is contributed by suppressing the activation of nuclear transcription factor- (NF-) κB and mitogen-activated protein kinase (MAPK) signaling cascades including p65, inhibition of NF-κB (IκB), extracellular signal-regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK), and p38. Therefore, these results demonstrated that diterpenoids 1 and 3 obtained from C. orbiculatus may be considered a potential candidate for the treatment of inflammatory diseases.

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Diarylheptanoids from Curcuma phaeocaulis Suppress IL-6-Induced STAT3 Activation

Cited by 9 publications

References 46 publications

Icaritin inhibits glioblastoma cell viability and glycolysis by blocking the IL‐6/Stat3 pathway

Icaritin inhibits glioblastoma cell viability and glycolysis by blocking the IL‐6/Stat3 pathway

TCMIO: A Comprehensive Database of Traditional Chinese Medicine on Immuno-Oncology

Anti-Inflammatory Activity of Diterpenoids fromCelastrus orbiculatusin Lipopolysaccharide-Stimulated RAW264.7 Cells

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