Diarylheptanoids from Alnus viridis ssp. viridis and Alnus glutinosa: Modulation of Quorum Sensing Activity in Pseudomonas aeruginosa

Abstract

Diarylheptanoids from the barks of ssp. (green alder) and (black alder) were explored for anti-quorum sensing activity. Chemicals with anti-quorum sensing activity have recently been examined for antimicrobial applications. The anti-quorum sensing activity of the selected diarylheptanoids was determined using two biosensors, namely PAO1 and CV026. Although all of the investigated compounds negatively influenced the motility of PAO1, four were able to inhibit biofilm formation of this human opportunistic pathogen for 40-70 %. Three of the diarylheptanoids (, and ) negatively influenced the biosynthesis of pyocyanin, which is under the control of quorum sensing. Platyphyllenone () and hirsutenone () were able to inhibit the biosynthesis of violacein in CV026, with being able to inhibit the synthesis of both biopigments. Only one of the tested diarylheptanoids () was shown to significantly decrease the production of acyl homoserine lactones (AHL) in PAO1, more specifically, production of the long chain N-(3-oxododecanoyl)-l-HSL. On the other side, four diarylheptanoids (-) significantly reduced the synthesis of 2-alkyl-4-quinolones, part of the quinolone-mediated signaling system. To properly assess therapeutic potential of these compounds, their antiproliferative effect on normal human lung fibroblasts was determined, with doses affecting cell proliferation between 10 and 100 µg/mL. This study confirms that the barks of green and black alders are rich source of phytochemicals with a wide range of biological activities that could further be exploited as natural agents against bacterial contaminations and infections.

<p>Quorum Quenching: A Potential Target for Antipseudomonal Therapy</p>

“…Pyocyanin secretion, the expression of genes lasB, rhlA, lasI, lasR, rhlI, and rhlR genes and production of biofilm [160] Manilkara zapota Pyocyanin, protease and elastase secretion and production of biofilm [151] Diarylheptanoids from the barks of Alnus viridis and Alnus glutinosa Pyocyanin secretion, twitching motility and production of biofilm [161] Gingerol Exoprotease, rhamnolipid and pyocyanin secretion and biofilm formation [162] Diterpene phytol Pyocyanin secretion, twitching motility and production of biofilm [163] Dovepress antibiotics can reduce the pathogenic factors' expression of P. aeruginosa such as the diminished of exotoxin A secretion, pyocyanin, protease, DNase, and phospholipase C, as well as significantly removing the QS activity of P. aeruginosa. 125 In addition, SICs of tobramycin could block the expressions of rhlI and rhlR genes by decreasing C4-HSL generation.…”

Plant-Derived Inhibitors of AHL-Mediated Quorum Sensing in Bacteria: Modes of Action

“…Curcumin inhibits violacein biosynthesis in C. violaceum as well as virulence factor production in Vibrio sp., S. marcescens and P. aeruginosa [24]. In Rudrappa and Bais’ study [80] this compound effectively inhibited biofilm formation, pyocyanin biosynthesis, elastase/protease activity, and AHL production in P. aeruginosa and as a consequence of this, reduced its pathogenicity in plant ( Arabidopsis thaliana ) and animal ( Caenorhabditis elegans ) infection models [80].…”

Aromatherapeutic essential oils and their pharmaceutical combinations: Tools for inhibition of quorum sensing activity and biofilm formation of human pathogens

“…Moreover, biofilm formation represents the main indicator of bacterial infection, especially those caused by devices such as catheters, prosthetic valves, orthopedic devices, etc. Therefore, the eradication of the biofilm matrix from these kinds of surfaces becomes extremely difficult (Li et al, 2018;Vipin, Mujeeburahiman, Ashwini, Arun, & Rekha, 2019;Yap et al, 2014;Zhang et al, 2019;Zhang et al, 2018).…”