Diarrhetic shellfish poisoning (DSP) associated with a sub-surface bloom of Dinophysis norvegica in Bedford Basin, eastern Canada

Rao, DV Subba; Pan, Youlian; Zitko, V.; Bugden, Gary; MacKeigan, K.

doi:10.3354/meps097117

Cited by 42 publications

(32 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…growth was concluded to be thermal stratification that lasted for over 2 weeks (Delmas et al, 1992). In Bedford Basin, eastern Canada, D. norvegica bloomed when a strong density gradient suppressed turbulence allowing the cells to concentrate (Subba Rao et al, 1993). Neither study found that Dinophysis blooms would depend on the availability of dissolved nutrients.…”

Section: Introductionmentioning

confidence: 94%

See 1 more Smart Citation

Survival and photosynthetic activity of different Dinophysis acuminata populations in the northern Baltic Sea

Setälä

Autio²,

Kuosa³

et al. 2005

Harmful Algae

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 94%

“…Delmas et al, 1992;Subba Rao et al, 1993;Carpenter et al, 1995;Figueroa et al, 1998;Gisselson et al, 2002;Godhe et al, 2002). The conditions leading to Dinophysis spp.…”

Section: Introductionmentioning

confidence: 99%

Survival and photosynthetic activity of different Dinophysis acuminata populations in the northern Baltic Sea

Setälä

Autio²,

Kuosa³

et al. 2005

Harmful Algae

View full text Add to dashboard Cite

“…The most regularly measured DSP toxin in Dinophysis is OA and its derivatives DTX-1 and DTX-2 (e.g., Cembella 1989;Subba Rao et al 1993;Morono et al 2003). In this study, no OA was found in the samples, which corresponds to the finding of Goto et al (2000), who did not find OA in their Baltic Dinophysis norvegica cells.…”

Section: Discussionmentioning

confidence: 99%

“…Among harmful algal bloom events, diarrhetic shellfish poisoning (DSP) syndrome in humans is caused mainly by representatives of the genus Dinophysis (e.g., Yasumoto et al 1985;Subba Rao et al 1993) after ingestion of shellfish containing Dinophysis toxins.…”

mentioning

confidence: 99%

Pectenotoxin-2 and dinophysistoxin-1 in suspended and sedimenting organic matter in the Baltic Sea

et al. 2006

View full text Add to dashboard Cite

Dinophysis acuminata, D. norvegica, and D. rotundata occur regularly in the Baltic Sea summer plankton. They are known to produce diarrhetic shellfish poisoning (DSP) toxins in coastal areas worldwide, but so far, evidence from the Baltic Sea is scarce, and the fate and transfer of their toxins in the ecosystem is poorly known. Occurrence of Dinophysis and DSP toxins was studied on the southwest coast of Finland in late July-September 2004 by sampling the water column down to the thermocline. DSP toxin profiles were analyzed using highperformance liquid chromatography-mass spectrometry from the thermocline sample from material collected with a sediment trap. Maximum abundances of D. acuminata (7,280 cells L 21 ) and D. rotundata (880 cells L 21 ) were above the thermocline, but D. norvegica (maximum 200 cells L 21 ) was most abundant in the thermocline region. Pectenotoxin-2 (PTX-2) was found during the entire study period. Cellular PTX-2 content in Dinophysis varied between 1.6 and 19.9 pg PTX-2 cell 21 . Dinophysistoxin-1 (DTX-1) was found in samples after mid-August in concentrations ranging from 0.2 to 149 pg DTX-1 cell 21 . PTX-2 and DTX-1 were found in all sediment trap samples. The estimated sedimentation rate of PTX-2 was 0 to 15.4 ng m 22 d 21 and DTX-1 0 to 190 ng m 22 d 21 , corresponding ,0.01% of PTX-2 and 1% of DTX-1 of the integrated water column DSP pool during a 6-week period. Sedimenting organic matter did not contain intact Dinophysis cells, but copepod fecal pellets found in the sediment traps indicated that fecal pellets are the major pathway of DSP toxins to the bottom. The major fraction of the PTX-2 and DTX-1 was either decomposed in the water column or transferred to higher trophic levels in the planktonic food chain.

show abstract

“…The occurrence of Dinophysis spp., especially Dinophysis acuta, D. acuminata and Dinophysis fortii, have been linked to the accumulation of OA group toxins and DSP in Asia (Yasumoto et al, 1978;Mak et al, 2005), North America (Subba Rao et al, 1993;Campbell et al, 2010), South America (Zhao et al,1993;Garcia et al, 2004), Australasia (MacKenzie et al, 1998Madigan et al, 2006), North of Europe (Torgersen et al, 2005;Turrell and Stobo, 2007;Fux et al, 2009) and South of Europe (Boni et al, 1993;Reguera et al, 1993;Gago-Martinez et al, 1993;Mouratidou et al, 2006). DSP symptoms include nausea, vomiting, diarrhea, and abdominal pain.…”

Section: Introductionmentioning

confidence: 99%

Toxin profiles of five geographical isolates of Dinophysis spp. from North and South America

Fux

Smith

Tong

et al. 2011

Toxicon

View full text Add to dashboard Cite

a b s t r a c tMarine dinoflagellates of the genus Dinophysis can produce toxins of the okadaic acid (OA) and pectenotoxin (PTX) groups. These lipophilic toxins accumulate in filter-feeding shellfish and cause an illness in consumers called diarrhetic shellfish poisoning (DSP). In 2008, a bloom of Dinophysis led to the closure of shellfish harvesting areas along the Texas coast, one of the first DSP-related closures in the U.S. This event resulted in a broad study of toxin production in isolates of Dinophysis spp. from U.S. waters. In the present study, we compared toxin profiles in geographical isolates of Dinophysis collected in the U.S. (Eel Pond, Woods Hole MA; Martha's Vineyard, MA; and Port Aransas Bay, Texas), and in those from Canada (Blacks Harbour, Bay of Fundy) and Chile (Reloncavi Estuary), when cultured in the laboratory under the same conditions. For each isolate, the mitochondrial cox1 gene was sequenced to assist in species identification. Strains from the northeastern U.S. and Canada were all assigned to Dinophysis acuminata, while those from Chile and Texas were most likely within the D. acuminata complex whereas precise species designation could not be made with this marker. Toxins were detected in all Dinophysis isolates and each isolate had a different profile. Toxin profiles of isolates from Eel Pond, Martha's Vineyard, and Bay of Fundy were most similar, in that they all contained OA, DTX1, and PTX2. The Eel Pond isolate also contained OA-D8 and DTX1-D7, and low levels (unconfirmed structurally) of DTX1-D8 and DTX1-D9. D. acuminata from Martha's Vineyard produced DTX1-D7, along with OA, DTX1, and PTX2, as identified in both the cells and the culture medium. D. acuminata from the Bay of Fundy produced DTX1 and PTX2, as found in both cells and culture medium, while only trace amounts of OA were detected in the medium. The Dinophysis strain from Texas only produced OA, and the one from Chile only PTX2, as confirmed in both cells and culture medium.Published by Elsevier Ltd.

show abstract

Diarrhetic shellfish poisoning (DSP) associated with a sub-surface bloom of Dinophysis norvegica in Bedford Basin, eastern Canada

Cited by 42 publications

References 16 publications

Survival and photosynthetic activity of different Dinophysis acuminata populations in the northern Baltic Sea

Survival and photosynthetic activity of different Dinophysis acuminata populations in the northern Baltic Sea

Pectenotoxin-2 and dinophysistoxin-1 in suspended and sedimenting organic matter in the Baltic Sea

Toxin profiles of five geographical isolates of Dinophysis spp. from North and South America

Contact Info

Product

Resources

About