Diarrheagenic Escherichia coli and Their Antibiotic Resistance Patterns in Dairy Farms and Their Microbial Ecosystems

Aditya, Arpita; Tabashsum, Zajeba; Martinez, Zabdiel Alvarado; Tung, Chuan Wei; Suh, Grace; Nguyen, Phuong L.; Biswas, Debabrata

doi:10.1016/j.jfp.2023.100051

Cited by 5 publications

(11 citation statements)

References 52 publications

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“…It can be pathogenic and non-pathogenic. 10 Non-pathogenic strains are part of the normal flora of the gut, aiding the hosts by producing vitamin K2 and preventing the establishment of pathogenic bacteria in the intestine E. coli can be differentiated into 190 serotypes (serogroups) based on somatic (O), capsular (K), and flagellar (H) antigens. About 80 different capsular polysaccharide (K) antigens are present.…”

Section: Common Causes Of Bacterial Infections In Dairy Calvesmentioning

confidence: 99%

A Comprehensive Review of the Common Bacterial Infections in Dairy Calves and Advanced Strategies for Health Management

Robi,

Mossie,

Temteme

2024

VMRR

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Dairy farming faces a significant challenge of bacterial infections in dairy calves, which can have detrimental effects on their health and productivity. This review offers a comprehensive overview of the most prevalent bacterial infections in dairy calves, including Escherichia coli, Salmonella typhimurium, Salmonella dublin, Salmonella enterica, Clostridium perfringens, Pasteurella multocida, Listeria monocytogenes, Mycoplasma bovis , and Haemophilus somnus . These pathogens can cause various clinical signs and symptoms, leading to diarrhea, respiratory distress, septicemia, and even mortality. Factors such as management practices, environmental conditions, and herd health influence the incidence and severity of the infections. Efficient management and prevention strategies include good colostrum and nutrient feeding, early detection, appropriate treatment, hygiene practices, and supportive care. Regular health monitoring and diagnostic tests facilitate early detection and intervention. The use of antibiotics should be judicious to prevent antimicrobial resistance and supportive care such as fluid therapy and nutritional support promotes recovery. Diagnostic methods, including immunological tests, culture, polymerase chain reaction (PCR), and serology, aid in the identification of specific pathogens. This review also explores recent advancements in the diagnosis, treatment, and prevention of bacterial infections in dairy calves, providing valuable insights for dairy farmers, veterinarians, and researchers. By synthesizing pertinent scientific literature, this review contributes to the development of effective strategies aimed at mitigating the impact of bacterial infections on the health, welfare, and productivity of young calves. Moreover, more research is required to enhance the understanding of the epidemiology and characterization of bacterial infections in dairy calves.

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Section: Common Causes Of Bacterial Infections In Dairy Calvesmentioning

confidence: 99%

A Comprehensive Review of the Common Bacterial Infections in Dairy Calves and Advanced Strategies for Health Management

Robi,

Mossie,

Temteme

2024

VMRR

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“…Multiple samples (between 5 and 15) from the same category were considered as technical replicates. All samples were aseptically collected, transported, and processed to the laboratory for analysis following the method previously published by our research group [6]. a A wide variety of produce samples were collected from all farms including garden vegetables, fruits, herbs, spices, etc.…”

Section: Sample Collectionmentioning

confidence: 99%

“…The collected samples underwent processing and enrichment, with the selective isolation of presumptive diarrheagenic E. coli based on cultural characteristics, following the protocol established by our group [6,7]. In summary, 1 g of each solid environmental sample (Table 1) was combined with 25 mL of 1× PBS (pH 7.4).…”

Section: Presumptive Isolation Of Diarrheagenic E Colimentioning

confidence: 99%

“…The presumptive isolates of diarrheagenic E. coli underwent further confirmation and identification of specific virulence genes using designated primers (Table 2), following a previously published protocol [6]. In brief, glycerol stocks were revitalized by streaking on LB agar and aerobic incubation at 37 • C. A colony from the presumptive isolation was selected and fully suspended in 50 µL PBS.…”

Section: Confirmation Of E Coli and Identifying Their Virulent Typesmentioning

confidence: 99%

“…These pathogens colonize in these farm animal intestines as normal microbiota and contaminate the animal food products during inappropriate processing. In addition to contaminating animal food products, they can contaminate soil, water, and grasses/plants and survive for a long time in soil, water, and plants through fecal runoff [6,7]. Given the numbers of foodborne illnesses with the pathogen STEC, the economic impacts are more than USD 1.6 billion [8].…”

Section: Introductionmentioning

confidence: 99%

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Dominance of Diarrheagenic E. coli Virulent Types in Integrated Crop–Livestock Farms and Their Antibiotic Resistance Patterns

Aditya,

Julianingsih,

Tabashsum

et al. 2024

Zoonotic Diseases

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Microbial ecology on integrated crop–livestock farms (ICLFs) can impact food safety through pathogen transfer between animals and crops. Recent reports of pathogen-contaminated products sold in local organic retail, roadside, and farmers markets highlight the need for assessment of the ecological patterns of bacterial pathogens. This study investigated the prevalence and antibiotic resistance of the virulent type of diarrheagenic E. coli in ICLFs. Over two years, 2973 samples from ICLFs and markets in Maryland and Washington DC were analyzed. Diarrheagenic E. coli was found in 4.30% (128/2973) of collected samples, with a higher isolation rate in environmental (4.42%, 59/1332) and produce (4.20%, 69/1641) samples. Overall, livestock bedding materials had the highest prevalence (8.51%, 4/47). Post-harvest produce exhibited a lower contamination rate of 1.32% (10/756), whereas pre-harvest produce had a higher incidence with 6.67% contamination (59/885), indicating the presence of E. coli. Alarmingly, 92.30% (72/78) of pathogenic E. coli isolates were resistant to common antibiotics. The findings highlight potential risks associated with integrated farming practices and emphasize the importance of safe harvesting and post-harvesting measures, particularly in the context of the growing popularity of local integrated farming. Implementing precautions at on-farm and market levels is crucial to mitigate the risk of antibiotic-resistant E. coli-related enteric illnesses, safeguarding both consumers and the integrity of integrated farming systems.

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Animal-originated foods as Potential sources of multidrug-resistant diarrheagenic E. coli Pathotypes in Kayseri, Türkiye

Heybet,

Ozkaya,

Gundog

et al. 2024

Food Control

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Diarrheagenic Escherichia coli and Their Antibiotic Resistance Patterns in Dairy Farms and Their Microbial Ecosystems

Cited by 5 publications

References 52 publications

A Comprehensive Review of the Common Bacterial Infections in Dairy Calves and Advanced Strategies for Health Management

A Comprehensive Review of the Common Bacterial Infections in Dairy Calves and Advanced Strategies for Health Management

Dominance of Diarrheagenic E. coli Virulent Types in Integrated Crop–Livestock Farms and Their Antibiotic Resistance Patterns

Animal-originated foods as Potential sources of multidrug-resistant diarrheagenic E. coli Pathotypes in Kayseri, Türkiye

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