Diaporthe orixae sp. nov., an endophytic species isolated from Orixa japonica in southern China

Abstract

In this study, a novel endophytic fungus, Diaporthe orixae, and a new host record of Diaporthe caryae were obtained from a medicinal plant, Orixa japonica in southern China. Phylogenetic analyses based on a combination of ITS, tef1-α, β-tub, cal, and his3 datasets confirmed that D. orixae is a distinct species, which is sister to D. sackstonii (BRIP 54669b). Diaporthe orixae sp. nov. differs from D. sackstonii in having larger conidiomata and smaller conidiogenous cells and alpha conidia on morphological examination. The new species and new host record are reported with taxonomic descriptions and illustrations.

Structure-Based Virtual Screening of Natural Products and Optimization for the Design and Synthesis of Novel CeCht1 Inhibitors as Nematicide Candidates

“…Furthermore, the natural products with potential were picked for further research regarding synthetic difficulty and structural modifiability space. The natural product lunidonine (Figure ), which was previously reported to have nematicidal activity against Bursaphelenchus xylophilus (LC 50 = 391.5 μg/mL) and Meloidogynein incongnita (LC 50 = 23.9 μg/mL), exhibited certain inhibitory activity against several chitinases, specifically the nematode chitinase Ce Cht1 (Table ), and was chosen to be the lead compound for the subsequent study.…”

Insights from the Endophytic Fungi in Amphisphaeria (Sordariomycetes): A. orixae sp. nov. from Orixa japonica and Its Secondary Metabolites

“…Orixa japonica is a plant of the family Rutaceae and mainly distributed in China, Korea, and Japan [ 5 ]. It is a traditional folk medicine that is frequently used to cure diseases or ease symptoms, such as clearing heat and dampness, relieving cough, and acting as an analgesic for stomach pain and rheumatic joint pain [ 6 ]. Its extract has pharmacological and biological activities [ 7 ].…”

“…In contrast, studies on endophytic fungi of O. japonica are relatively scarce or neglected. From the stem of O. japonica , Lu et al [ 6 ] isolated the novel endophytic fungus Diaporthe orixae and discovered a new host record for Diaporthe caryae . At the same time, Japanese scholars similarly obtained an endophytic fungus belonging to Diaporthe from the leaves of O. japonica and inoculated it on MEA medium to obtain six polyketide derivatives as secondary metabolites from large-scale fermentation [ 12 ].…”

“…Since 2020, our research group has been focusing on the diversity of endophytic fungi of Orixa japonica [ 6 ]. In this study, a new endophytic fungus, Amphisphaeria orixae , was isolated from O. japonica and is introduced based on multi-gene phylogenetic analyses and morphological evidence.…”

“…A. orixae is the first endophytic fungus of Amphisphaeria that forms a hyphomycetous asexual morph and is unique in undergoing thallic or blastic conidiogenesis and having polymorphic conidia. Thirteen secondary metabolites were identified from the rice fermentation product of A. orixae, including a new isocoumarin, (R)-4,6,8-trihydroxy-5-methylisochroman-1-one (1), and 12 known compounds (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). Their chemical structures are depicted in Figure 4 and were established using 1D-and 2D-NMR, mass spectrometry, and chemical computations.…”