Diaphragm muscle sarcopenia into very old age in mice

Vang, Pangdra; Vasdev, Amrit; Zhan, Wen Zhi; Gransee, Heather M.; Sieck, Gary C.; Mantilla, Carlos B.

doi:10.14814/phy2.14305

Cited by 18 publications

(16 citation statements)

References 59 publications

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“…Mice were weighed and placed into one of two plethysmograph chambers with recordings done in parallel and groups (sham, OVX) counterbalanced. WBP recordings and analysis were adapted from previously described [ 40 – 42 ]. Briefly, all mice were acclimated for 10 min, followed by a 45 min recording session during eupnea, and 5 min recording session during HH for a total duration of 1 hr.…”

Section: Methods and Experimental Designmentioning

confidence: 99%

Impact of estrogen deficiency on diaphragm and leg muscle contractile function in female mdx mice

et al. 2021

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Female carriers of Duchenne muscular dystrophy (DMD) presenting with DMD symptomology similar to males with DMD, such as skeletal muscle weakness and cardiomyopathy, are termed manifesting carriers. There is phenotypic variability among manifesting carriers including the age of onset, which can range from the first to fourth decade of life. In females, estrogen levels typically begin to decline during the fourth decade of life and estrogen deficiency contributes to loss of muscle strength and recovery of strength following injury. Thus, we questioned whether the decline of estrogen impacts the development of DMD symptoms in females. To address this question, we studied 6–8 month-old homozygous mdx female mice randomly assigned to a sham or ovariectomy (OVX) surgical group. In vivo whole-body plethysmography assessed ventilatory function and diaphragm muscle strength was measured in vitro before and after fatigue. Anterior crural muscles were analyzed in vivo for contractile function, fatigue, and in response to eccentric contraction (ECC)-induced injury. For the latter, 50 maximal ECCs were performed by the anterior crural muscles to induce injury. Body mass, uterine mass, hypoxia-hypercapnia ventilatory response, and fatigue index were analyzed by a pooled unpaired t-test. A two-way ANOVA was used to analyze ventilatory measurements. Fatigue and ECC-injury recovery experiments were analyzed by a two-way repeated-measures ANOVA. Results show no differences between sham and OVX mdx mice in ventilatory function, strength, or recovery of strength after fatigue in the diaphragm muscle or anterior crural muscles (p ≥ 0.078). However, OVX mice had significantly greater eccentric torque loss and blunted recovery of strength after ECC-induced injury compared to sham mice (p ≤ 0.019). Although the results show that loss of estrogen has minimal impact on skeletal muscle contractile function in female mdx mice, a key finding suggests that estrogen is important in muscle recovery in female mdx mice after injury.

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Section: Methods and Experimental Designmentioning

confidence: 99%

Impact of estrogen deficiency on diaphragm and leg muscle contractile function in female mdx mice

et al. 2021

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“…Sarcopenia is a progressive and generalised skeletal muscle disorder characterized by a decrease of muscle quality, quantity and function [12e16]. This process is particularly evident in respiratory muscles [1,15] where it impairs the ability to produce appropriate tidal volumes [17] and to perform high force expulsive airway clearance manoeuvres [18]. Sarcopenia has been associated with many clinical adverse outcomes [13,14,19e21], however only one study has evaluated the association between reduced muscle mass and extubation failure so far.…”

Section: Introductionmentioning

confidence: 99%

Influence of reduced muscle mass and quality on ventilator weaning and complications during intensive care unit stay in COVID-19 patients

et al. 2022

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Background & Aims: sarcopenia, a loss of muscle mass, quality and function, which is particularly evident in respiratory muscles, has been associated with many clinical adverse outcomes. In this study, we aimed at evaluating the role of reduced muscle mass and quality in predicting ventilation weaning, complications, length of intensive care unit (ICU) and of hospital stay and mortality in patients admitted to ICU for SARS-CoV-2-related pneumonia. Methods: this was an observational study based on a review of medical records of all adult patients admitted to the ICU of a tertiary hospital in Milan and intubated for SARS-CoV-2-related pneumonia during the first wave of the COVID-19 pandemic. Muscle mass and quality measurement were retrieved from routine thoracic CT scans, when sections passing through the first, second or third lumbar vertebra were available. Results: a total of 81 patients were enrolled. Muscle mass was associated with successful extubation (OR 1.02, 95% C.I. 1.00 - 1.03, p = 0.017), shorter ICU stay (OR 0.97, 95% C.I. 0.95 - 0.99, p = 0.03) and decreased hospital mortality (HR 0.98, 95% C.I. 0.96 – 0.99, p = 0.02). Muscle density was associated with successful extubation (OR 1.07, 95% C.I. 1.01 - 1.14; p = 0.02) and had an inverse association with the number of complications in ICU (Β -0.07, 95% C.I. -0.13 - -0.002, p = 0.03), length of hospitalization (Β -1.36, 95% C.I. -2.21 - -0.51, p=0.002) and in-hospital mortality (HR 0.88, 95% C.I. 0.78 – 0.99, p = 0.046). Conclusions: leveraging routine CT imaging to measure muscle mass and quality might constitute a simple, inexpensive and powerful tool to predict survival and disease course in patients with COVID-19. Preserving muscle mass during hospitalisation might have an adjuvant role in facilitating remission from COVID-19.

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“…The condition of muscle fiber atrophy and weakness that occurs in respiratory muscles along with systemic skeletal muscle with aging is known as respiratory sarcopenia (1)(2)(3)(4). Respiratory sarcopenia causes deterioration of respiratory force generation (5) and pulmonary function (3,6), which then adversely affect activities of daily living and quality of life (3,(5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

Respiratory Sarcopenia and Sarcopenic Respiratory Disability: Concepts, Diagnosis, and Treatment

Nagano

Wakabayashi

Maeda

et al. 2021

The Journal of nutrition, health and aging

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The condition of muscle fiber atrophy and weakness that occurs in respiratory muscles along with systemic skeletal muscle with age is known as respiratory sarcopenia. The Japanese Working Group of Respiratory Sarcopenia of the Japanese Association of Rehabilitation Nutrition narratively reviews these areas, and proposes the concept and diagnostic criteria. We have defined respiratory sarcopenia as "wholebody sarcopenia and low respiratory muscle mass followed by low respiratory muscle strength and/or low respiratory function." Respiratory sarcopenia can be caused by various factors such as aging, decreased activity, undernutrition, disease, cachexia, and iatrogenic causes. We have also created an algorithm for diagnosing respiratory sarcopenia. Respiratory function decreases with age in healthy older people, along with low respiratory muscle mass and strength. We have created a new term, "Presbypnea," meaning a decline in respiratory function with aging. Minor functional respiratory disability due to aging, such as that indicated by a modified Medical Research Council level 1 (troubled by shortness of breath when hurrying or walking straight up hill), is an indicator of presbypnea. We also define sarcopenic respiratory disability as "a disability with deteriorated respiratory function that results from respiratory sarcopenia." Sarcopenic respiratory disability is diagnosed if respiratory sarcopenia is present with functional disability. Cases of respiratory sarcopenia without functional disability are diagnosed as "at risk of sarcopenic respiratory disability." Functional disability is defined as a modified Medical Research Council grade of 2 or more. Rehabilitation nutrition, treatment that combines rehabilitation and nutritional management, may be adequate to prevent and treat respiratory sarcopenia and sarcopenic respiratory disability.

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Diaphragm muscle sarcopenia into very old age in mice

Cited by 18 publications

References 59 publications

Impact of estrogen deficiency on diaphragm and leg muscle contractile function in female mdx mice

Impact of estrogen deficiency on diaphragm and leg muscle contractile function in female mdx mice

Influence of reduced muscle mass and quality on ventilator weaning and complications during intensive care unit stay in COVID-19 patients

Respiratory Sarcopenia and Sarcopenic Respiratory Disability: Concepts, Diagnosis, and Treatment

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