Diaphonization: A Recipe to Study Teeth

Rehman, Kiran; Khan, Farhan Raza; Habib, Sadia

doi:10.5005/jp-journals-10024-1670

Cited by 9 publications

(3 citation statements)

References 12 publications

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“…This direction of diaphonization was practically used as one of the methods of preparing future dentists in institutions of higher education. The success of this experience allows us to consider diaphonization as a promising method for the production of educational anatomical preparations [7].…”

Section: методи дослідженняmentioning

confidence: 95%

Diaphonization as a Method of Modern Morphological Research

Vovk¹,

Ionov²,

Lyutenko³

et al. 2022

CAOS

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In the article diaphonization is discussed as one of the methods of morphological research in the direction of the study of blood supply to the brain. The history of the research of this method is analyzed, the areas of its application are classiﬁ ed according to the research objects. We have highlighted the advantages of using the diaphonization method for research and educational purposes. Also basing on the standard diaphonization protocol, an updated version of it was created, adapted speciﬁ cally for the study of cerebral blood supply. In the protocol improved by us, the proportions of the relevant substances have been changed, certain stages of the preparation have been excluded or quantitatively changed; changes were made taking into account the histological and histochemical speciﬁ city of brain tissues and with the aim of maximally improving the visibility of the corresponding structures in the preparation. In addition to the description of the experimental study and the modiﬁ ed protocol, we have outlined some separate methods of using readymade preparations in the educational process of students, as well as their usage in collaboration with modern educational systems of three- anatomy (Artec Space Spider scanner, Anatomage virtual anatomical table, etc.) and others research perspectives.

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Section: методи дослідженняmentioning

confidence: 95%

Diaphonization as a Method of Modern Morphological Research

Vovk¹,

Ionov²,

Lyutenko³

et al. 2022

CAOS

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“…adecuada, autores como Kiran Rehman, 27 Vertucci, 30 Pecora, 31 Castellucci 32 , han reportado el uso de ácido nítrico al 5% en dientes con y sin endodoncia, para controlar el fenómeno de contracción del tejido dental orgánico por el proceso de desmineralización. 1 Autores como Labarta 29 y Parek H 3 , emplearon Ácido Nítrico al 5% por 72 horas con recambios cada 24 horas para darle estabilidad al tejido, pues cuanto más rápida es la acción del agente descalcificante, mayor es el daño al tejido 1 .…”

Section: A) B) C) D) E) F) G) H)unclassified

Protocolo para diafanización dental

Cano¹,

Quiroga

Morales³

et al. 2023

Rev Estomatol

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Antecedentes: La Diafanización dental es una técnica que permite transparentar los dientes haciendo visible la anatomía interna de los conductos radiculares ofreciendo una herramienta pedagógica económica y confiable; sin embargo, la literatura no reporta protocolos estandarizados para obtener una diafanización dental predecible. Objetivo: Obtener un protocolo estandarizado para diafanización dental, como modelo educativo, a partir de la revisión de la literatura y la realización de un estudio piloto. Materiales y Métodos: Se realizó una búsqueda sistemática en las bases de datos Scopus y Medline con los términos Mesh "root canal", "diaphonization", "clearing", "morphology" anatomy", y se estructuró una tabla de extracción con las variables más representativas para establecer las 3 fases de la Diafanización, a. Descalcificación, se evaluó Ácido Nítrico 5% (HNO3), Ácido Fórmico 10% (CH2O2) y EDTA 10%, b. Deshidratación, se empleó Alcoholes etílicos ascendentes, c. Clarificación, se evaluó Metil Salicilato y Aceite de Inmersión Sintético. Se seleccionaron 54 dientes, 36 sin endodoncia, y 18 con endodoncia, y se distribuyeron en dos grupos: Grupo A. Dientes sin endodoncia, Grupo B. Dientes con endodoncia, constituidos por 18 subgrupos que estaban definidos de acuerdo al tipo de descalcificante, momento de aplicación medio de contraste y medio de Clarificación. Resultados: El Ácido Nítrico al 5% pese a que fue el más corrosivo, permitió el mayor flujo y accesibilidad para el medio de contraste (Tinta China) en especímenes sin endodoncia. De igual manera, el Ácido Fórmico al 10%, preservó la estructura de los dientes tratados endodónticamente. El Metil Salicilato como clarificante, brindó mejores resultados visuales alcanzando una mayor transparencia. Conclusiones: El desarrollo de un estudio piloto para estandarizar técnicas de diafanización en odontología, permite la estructuración de un protocolo educativo que posibilita conocer la gran variabilidad anatómica de los dientes y la comprensión y análisis de los dientes que han sido tratados endodóncicamente, aportando una herramienta pedagógica para la comprensión de la anatomía radicular. El uso de Ácido Fórmico al 10%, en dientes con tratamiento de endodoncia y de Ácido Nítrico 5% en dientes sin endodoncia, con una transparencia alcanzada por el uso del Metil Salicilato, muestran los mejores resultados visuales en anatomía y obturación endodóntica.

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“…Bioactive materials were filled in the transparent teeth, and the apical region was submerged in methylene blue solubilized PBS solution to evaluate dye leakage through tooth apex. Diaphonization protocol was followed as described by Rehman et al [30].…”

Section: Evaluation Of Micro-leakagementioning

confidence: 99%

Unravelling the effects of ibuprofen-acetaminophen infused copper-bioglass towards the creation of root canal sealant

Chitra¹,

Chandran²,

Ramadoss³

et al. 2022

Biomed. Mater.

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Impact towards the tuneable characteristics of bioactive glasses (BAG) has been explored; as there is no root canal sealant till date with ideal characteristics competent enough to manoeuvre the perplexing root canal architecture. Combeite, calcite and traces of cuprorivaite crystalline phases were validated for material formation, in which Cu 2P [XPS] peak authenticating the presence of copper in bioglass network (Cu-BAG). Spherical and platelet-like morphologies were observed and the grain size of Cu-BAG (~100 nm) was lesser as compared to BAG (~ 1 µm). These particle distributions impacted the porosity, and dominant non-bridging oxygens in Cu-BAG influences ionic dissolution, which subsequently enhanced the mineralization. These bioactive materials were loaded with acetaminophen and ibuprofen, corresponding organic moieties was confirmed through FT-IR. These drugs loaded bioactive materials exhibited tremendous anti-inflammatory and anti-microbial behavior with better sealing ability. Drug loaded bioglass paste filled in biomechanically prepared root canal was estimated for sealing potential, mineralization, micro leakage, and fracture resistance properties. Hydroxyl apatite growth was noted on the sealants, flower like protuberance confirmed the sealing potential of the prepared material. Bioglass exhibited promising characteristics required in a root canal sealant. This investigation is a step further towards tailoring the properties of bioactive materials as promising candidates in root canal obturation and thereof.

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Diaphonization: A Recipe to Study Teeth

Cited by 9 publications

References 12 publications

Diaphonization as a Method of Modern Morphological Research

Diaphonization as a Method of Modern Morphological Research

Protocolo para diafanización dental

Unravelling the effects of ibuprofen-acetaminophen infused copper-bioglass towards the creation of root canal sealant

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