Diapause-Associated Protein3 Functions as Cu/Zn Superoxide Dismutase in the Chinese Oak Silkworm (Antheraea pernyi)

Bi, Zhenle; Yang, Xiaoli; Yu, Wengong; Shu, Jianhong; Zhang, Yaozhou

doi:10.1371/journal.pone.0090435

Cited by 10 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found strong down-regulation of SOD1-vtg fusion in response to osmotic (NaCl) and oxidative stress (UV-B light), whereas we observed up-regulation of SOD-vtg under Cd, carbaryl and both microcystin treatments (Table S1). SOD-vtg is generally expressed in diapausing insects (Bi et al 2014) and crustaceans (Acton 2012) ); hence, the regulation of this gene under environmental perturbation indicates a potential alteration of the diapause process by environmental perturbations (e.g. Slusarczyk & Rybicka 2011).…”

Section: Resultsmentioning

confidence: 99%

Early transcriptional response pathways in Daphnia magna are coordinated in networks of crustacean‐specific genes

et al. 2017

View full text Add to dashboard Cite

Natural habitats are exposed to an increasing number of environmental stressors that cause important ecological consequences. However, the multifarious nature of environmental change, the strength and the relative timing of each stressor largely limit our understanding of biological responses to environmental change. In particular, early response to unpredictable environmental change, critical to survival and fitness in later life stages, is largely uncharacterized. Here, we characterize the early transcriptional response of the keystone species Daphnia magna to twelve environmental perturbations, including biotic and abiotic stressors. We first perform a differential expression analysis aimed at identifying differential regulation of individual genes in response to stress. This preliminary analysis revealed that a few individual genes were responsive to environmental perturbations and they were modulated in a stressor and genotype-specific manner. Given the limited number of differentially regulated genes, we were unable to identify pathways involved in stress response. Hence, to gain a better understanding of the genetic and functional foundation of tolerance to multiple environmental stressors, we leveraged the correlative nature of networks and performed a weighted gene co-expression network analysis. We discovered that approximately one-third of the Daphnia genes, enriched for metabolism, cell signalling and general stress response, drives transcriptional early response to environmental stress and it is shared among genetic backgrounds. This initial response is followed by a genotype- and/or condition-specific transcriptional response with a strong genotype-by-environment interaction. Intriguingly, genotype- and condition-specific transcriptional response is found in genes not conserved beyond crustaceans, suggesting niche-specific adaptation.

show abstract

Section: Resultsmentioning

confidence: 99%

Early transcriptional response pathways in Daphnia magna are coordinated in networks of crustacean‐specific genes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…minax , a microsomal GST (mGST, BmUnigene15924.co) and a Mn-SOD (BmUnigene23806.co) were up-regulated throughout diapause ( S5 Fig ). Likewise, members of the GST or SOD family were also up-regulated in other insects during diapause [ 32 , 56 , 57 ]. The up-regulation of these antioxidant proteins is speculated to protect individuals from oxidative damage in hypoxia/anoxia environments.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and metabolomic profiles of Chinese citrus fly, Bactrocera minax (Diptera: Tephritidae), along with pupal development provide insight into diapause program

et al. 2017

View full text Add to dashboard Cite

The Chinese citrus fly, Bactrocera minax (Enderlein), is a devastating citrus pest in Asia. This univoltine insect enters obligatory pupal diapause in each generation, while little is known about the course and the molecular mechanisms of diapause. In this study, the course of diapause was determined by measuring the respiratory rate throughout the pupal stage. In addition, the variation of transcriptomic and metabolomic profiles of pupae at five developmental stages (pre-, early-, middle-, late-, and post-diapause) were evaluated by next-generation sequencing technology and 1H nuclear magnetic resonance spectroscopy (NMR), respectively. A total of 4,808 genes were significantly altered in ten pairwise comparisons, representing major shifts in metabolism and signal transduction as well as endocrine system and digestive system. Gene expression profiles were validated by qRT-PCR analysis. In addition, 48 metabolites were identified and quantified by 1H NMR. Nine of which significantly contributed to the variation in the metabolomic profiles, especially proline and trehalose. Moreover, the samples collected within diapause maintenance (early-, middle-, and late-diapause) only exhibited marginal transcriptomic and metabolomic variation with each other. These findings greatly improve our understanding of B. minax diapause and lay the foundation for further pertinent studies.

show abstract

“…In insects, SOD1-null flies show markedly decreased lifespans and are highly susceptible to oxidation [ 27 ]. Diapause-associated protein 3 has a copper/zinc superoxide dismutase (Cu/Zn-SOD) domain and is involved in the regulation of diapause in Antheraea pernyi [ 28 ]. Hyphantria cunea SOD1 and SOD2 play a role in resistance to oxidative stress, and SOD2, in particular, plays a role with apo lipophorin III in apoptosis [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Superoxide Dismutases, SOD1 and SOD2, Play a Distinct Role in the Fat Body during Pupation in Silkworm Bombyx mori

et al. 2015

View full text Add to dashboard Cite

One way that aerobic biological systems counteract the generation of reactive oxygen species (ROS) is with superoxide dismutase proteins SOD1 and SOD2 that metabolize superoxide radicals to molecular oxygen and hydrogen peroxide or scavenge oxygen radicals produced by the extensive oxidation-reduction and electron-transport reactions that occur in mitochondria. We characterized SOD1 and SOD2 of Bombyx mori isolated from the fat body of larvae. Immunological analysis demonstrated the presence of BmSOD1 and BmSOD2 in the silk gland, midgut, fat body, Malpighian tubules, testis and ovary from larvae to adults. We found that BmSOD2 had a unique expression pattern in the fat body through the fifth instar larval developmental stage. The anti-oxidative functions of BmSOD1 and BmSOD2 were assessed by exposing larvae to insecticide rotenone or vasodilator isosorbide dinitrate, which is an ROS generator in BmN4 cells; however, exposure to these compounds had no effect on the expression levels of either BmSOD protein. Next, we investigated the physiological role of BmSOD1 and BmSOD2 under environmental oxidative stress, applied through whole-body UV irradiation and assayed using quantitative RT-PCR, immunoblotting and microarray analysis. The mRNA expression level of both BmSOD1 and BmSOD2 was markedly increased but protein expression level was increased only slightly. To examine the differences in mRNA and protein level due to UV irradiation intensity, we performed microarray analysis. Gene set enrichment analysis revealed that genes in the insulin signaling pathway and PPAR signaling pathway were significantly up-regulated after 6 and 12 hours of UV irradiation. Taken together, the activities of BmSOD1 and BmSOD2 may be related to the response to UV irradiation stress in B. mori. These results suggest that BmSOD1 and BmSOD2 modulate environmental oxidative stress in the cell and have a specific role in fat body of B. mori during pupation.

show abstract

Diapause-Associated Protein3 Functions as Cu/Zn Superoxide Dismutase in the Chinese Oak Silkworm (Antheraea pernyi)

Cited by 10 publications

References 27 publications

Early transcriptional response pathways in Daphnia magna are coordinated in networks of crustacean‐specific genes

Early transcriptional response pathways in Daphnia magna are coordinated in networks of crustacean‐specific genes

Transcriptomic and metabolomic profiles of Chinese citrus fly, Bactrocera minax (Diptera: Tephritidae), along with pupal development provide insight into diapause program

Superoxide Dismutases, SOD1 and SOD2, Play a Distinct Role in the Fat Body during Pupation in Silkworm Bombyx mori

Contact Info

Product

Resources

About