Dianthus Species (Carnation): In Vitro Culture and the Biosynthesis of Dianthalexin and Other Secondary Metabolites

Matern, Ulrich

doi:10.1007/978-3-662-30369-6_12

Cited by 4 publications

(1 citation statement)

References 76 publications

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“…This is in contrast to recent findings for AS from Catharanthus roseus, which was reported to have a native molecular weight of 145 kDa and to consist of two 67 kDa c~-subunits and two 25 kDa J3-subunits (Poulsen eta/., 1993). A third study of plant AS suggests that the partially purified Dianthus caryophyllus enzyme has a native molecular weight of 140 kDa and may be composed of two subunits of 68 and 72 kDa, respectively (Matern, 1994), which would be congruent with our data. The existence of 25 kD AS~ subunits in A. thaliana was deduced from E. coil functional complementation approaches and from Arabidopsis mutant analyses (Niyogi et al, 1993), although AS~ proteins from Arabidopsis have not been isolated.…”

Section: Discussionsupporting

confidence: 90%

Purification and cDNA cloning of anthranilate synthase from Ruta graveolens: modes of expression and properties of native and recombinant enzymes

et al. 1995

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SummaryRuta graveolens utilizes anthrenilete synthese (AS) for the synthesis both of tryptophan in primary metabolism and acridone alkaloids in secondary metabolism. AS has been purified from plants and cell cultures of R. graveolens 670-and 1700-fold, respectively. Glutamine-end ammoniadependent AS activities were strictly co-purified in all steps. Through cDNA cloning and complementetion of Escherichia coli deletion mutants defective for AS, it is shown that young Ruta plants express two genes for functional AS~ subunits, AS~ land AS(~2. The data indicate that AS~ from Ruta requires an AS~ subunit with a native molecular weight of 60-65 kDe for the glutaminedependent reaction. Protein synthesized in vitro from cloned cDNA is processed upon import into isolated chloroplasts, indicating that mature AS~ subunits are active in plastids in vivo. AS~I and AS(~2 are constitutively expressed in Ruta cell cultures, but AS~I steedy-stete mRNA levels are increased 100-fold 6 h subsequent to elicitation whereas AS~2 expression remains constitutive. Increased AS~I transcription corresponds to elicitorinduced alkaloid accumulation. The data indicate that Ruta regulates anthranilate flux into primary and secondary metabolism through differential regulation of AS genes specific to these pathways.

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Section: Discussionsupporting

confidence: 90%

Purification and cDNA cloning of anthranilate synthase from Ruta graveolens: modes of expression and properties of native and recombinant enzymes

et al. 1995

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Diethyl‐Ether Flower Washings of Dianthus cruentusGriseb. (Caryophyllaceae): Derivatization Reactions Leading to the Identification of New Wax Constituents

Dekić¹,

Ristić²,

Mladenović

et al. 2019

Chemistry & Biodiversity

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Some carnation species (Dianthus spp., Caryophyllaceae) exhibit a strong resistance to drought stress that is connected with the increased surface wax formation. Wax composition is unknown for the majority of Dianthus spp. Herein, mass spectral and gas chromatographic data, in combination with synthesis and chemical transformations (transesterification and synthesis of dimethyl disulfide adducts), enabled the identification of 151 constituents of diethyl‐ether washings of fresh flowers of Dianthus cruentus Griseb. from Serbia. The flower wax contained, along with the dominant ubiquitous long‐chain n‐alkanes, homologous series of n‐ and branched (iso‐ and anteiso‐) long‐chain hexyl alkanoates/alkenoates and alkyl/alkenyl benzoates. The branching position in the mentioned hexyl esters was probed by synthesis of esters of three isomeric hexanols that were spectrally characterized (1H‐ and 13C‐NMR, IR, MS). The washings also contained long‐chain (Z)‐ and (E)‐alkenes (C23−C35) with several different double bond regiochemistries. Fifty‐five of these constituents (eight hexyl esters, two benzoates, and forty‐five alkenes) were detected for the first time in Plantae, while ten of these represent completely new compounds. The rare occurrence of these wax constituents makes them possible chemotaxonomic markers of this particular Dianthus sp.

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The Involvement of Different Secondary Metabolites in Salinity Tolerance of Crops

Sytar

Mbarki

Živčák

et al. 2018

Salinity Responses and Tolerance in Plants, Volume 2

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Dianthus Species (Carnation): In Vitro Culture and the Biosynthesis of Dianthalexin and Other Secondary Metabolites

Cited by 4 publications

References 76 publications

Purification and cDNA cloning of anthranilate synthase from Ruta graveolens: modes of expression and properties of native and recombinant enzymes

Purification and cDNA cloning of anthranilate synthase from Ruta graveolens: modes of expression and properties of native and recombinant enzymes

Diethyl‐Ether Flower Washings of Dianthus cruentusGriseb. (Caryophyllaceae): Derivatization Reactions Leading to the Identification of New Wax Constituents

The Involvement of Different Secondary Metabolites in Salinity Tolerance of Crops

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