Diamine oxidase is involved in H2O2 production in the chalazal cells during barley grain filling

Asthir, Bavita; Duffus, C. M.; Smith, Rachel Charlotte; Spoor, W.

doi:10.1093/jexbot/53.369.677

Cited by 59 publications

(26 citation statements)

References 15 publications

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Section: Advances In Seed Biologymentioning

confidence: 99%

“…Hydrogen peroxide is suggested to participate in lignin deposition in the cell walls in a peroxidase-catalyzed reaction [100]. The involvement of a diamine oxidase in H 2 O 2 production has been demonstrated along with lignin deposition in the chalazal cells, in developing barley grains, in developing barley grains [100]. Production of ROS and their release in the surrounding medium are supposed to play a part in protecting the embryo against pathogens during seed imbibition [99].…”

Section: Advances In Seed Biologymentioning

confidence: 99%

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Free Radicals and Antioxidant System in Seed Biology

Pehlivan¹

2017

Advances in Seed Biology

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Reactive oxygen species (ROS) are involved in various development stages of seed biology. During seed desiccation, germination and aging, oxidative stress may increase in higher levels, leading to cellular damage and seed deterioration. Plant cells have antioxidant system, detoxifying enzymes and antioxidant compounds, that scavenge ROS, participating in seed survival. This antioxidant system has various roles in desiccation and germination of developing seeds, seed storability, and seed aging. On the other hand, ROS are accepted as molecules involving in cellular signaling, and having regulatory functions in seed development. ROS are also found to have roles in gene expression in early embryogenesis, dormancy and germination. Abscisic acid is a plant hormone and a signaling molecule in seed development and that is reported to have relationships with ROS. The objective of this article is to review the roles of ROS and the importance of antioxidant system in orthodox seeds, and to emphasize the dual efects of ROS in seed biology.

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Section: Advances In Seed Biologymentioning

confidence: 99%

Section: Advances In Seed Biologymentioning

confidence: 99%

Free Radicals and Antioxidant System in Seed Biology

Pehlivan¹

2017

Advances in Seed Biology

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“…In suberin-and lignin-rich cell walls, the cross-linking of phenolics is mediated by H 2 O 2 (Fry, 1998;Schweikert et al, 2000), a process that occurs in chalazal cells during the final stage of barley grain development (Cochrane, 2000;Asthir et al, 2002). ROS are intimately involved in PCD (Apel and Hirt, 2004;Van Breusegem et al, 2008).…”

Section: Inulin-type Fructans Accumulate In Transfer Cell Tissuesmentioning

confidence: 99%

Spatio-Temporal Dynamics of Fructan Metabolism in Developing Barley Grains

et al. 2014

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Barley (Hordeum vulgare) grain development follows a series of defined morphological and physiological stages and depends on the supply of assimilates (mainly sucrose) from the mother plant. Here, spatio-temporal patterns of sugar distributions were investigated by mass spectrometric imaging, targeted metabolite analyses, and transcript profiling of microdissected grain tissues. Distinct spatio-temporal sugar balances were observed, which may relate to differentiation and grain filling processes. Notably, various types of oligofructans showed specific distribution patterns. Levan-and graminan-type oligofructans were synthesized in the cellularized endosperm prior to the commencement of starch biosynthesis, while during the storage phase, inulin-type oligofructans accumulated to a high concentration in and around the nascent endosperm cavity. In the shrunken endosperm mutant seg8, with a decreased sucrose flux toward the endosperm, fructan accumulation was impaired. The tight partitioning of oligofructan biosynthesis hints at distinct functions of the various fructan types in the young endosperm prior to starch accumulation and in the endosperm transfer cells that accomplish the assimilate supply toward the endosperm at the storage phase.

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“…The supernatant was used as source of enzyme. DAO (EC 1.4.3.6) and PAO (EC 1.5.3.11) activities were assayed as described by Asthir et al (2002) using Put (for DAO) and Spd (for PAO) as substrates. Activities are expressed as nmol (D-pyrroline) min -1 g -1 FW for DAO and PAO.…”

Section: Dao and Pao Enzyme Assaysmentioning

confidence: 99%

Modulation of antioxidant defense system and polyamine catabolism in rice leaves under two planting conditions

Asthir

Kumari

2017

Cereal Research Communications

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Aerobic rice offers an attractive alternative approach over transplanting system as it consumes less water with low labour expenses. Flag leaf of six rice cultivars, viz. PR 120, PR 115, PR 116, Feng Ai Zan, PAU 201 and Punjab Mehak 1 was analysed for antioxidant defence mechanism and polyamine catabolism under the aerobic and the transplanting conditions. Ascorbate peroxidase (APX), guaiacol peroxidase (GPX), catalase (CAT), superoxide dismutase (SOD), diamine oxidase (DAO) and polyamine oxidase (PAO) activities increased gradually from tillering to anthesis stage and then declined towards maturity stage under both planting conditions. Apparently, contents of ascorbic acid, α-tocopherol, proline and polyamines (PAs) also revealed similar trend. The aerobic condition elevated activities of PAO, SOD as well as contents of PAs, lipid peroxide and H 2 O 2 whereas the transplanting condition had higher levels of APX, GPX, CAT and total antioxidant activities and contents of ascorbate, α-tocopherol and proline. Cultivars Feng Ai Zan, PR 115 and PR 120 exhibited superior tolerance over other cultivars by accumulating higher contents of PAs with increasing levels of PAO and SOD activities under the aerobic condition. However, under the transplanting condition PR 116 and PAU 201 showed higher activities of antioxidative enzymes with decreasing contents of lipid peroxide and H 2 O 2 . We infer that under the aerobic condition, enhancement of PAs and PAO activity enabled rice cultivars to tolerate oxidative stress, while under the transplanting condition, antioxidative defence system with decreasing of lipid peroxide content was closely associated with the protection of flag leaf by maintaining membrane integrity. In crux, results indicated that H 2 O 2 metabolic machinery was strongly up-regulated especially at the anthesis stage.

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Diamine oxidase is involved in H2O2 production in the chalazal cells during barley grain filling

Cited by 59 publications

References 15 publications

Free Radicals and Antioxidant System in Seed Biology

Free Radicals and Antioxidant System in Seed Biology

Spatio-Temporal Dynamics of Fructan Metabolism in Developing Barley Grains

Modulation of antioxidant defense system and polyamine catabolism in rice leaves under two planting conditions

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