Diamine Oxidase Activity During the Germinative and Post-Germinative Growth of the Embryonic Axis in Chickpea Seeds

Matilla, Ángel J.; García, S. García; Bueno, Milagros

doi:10.1023/a:1022325109218

Cited by 3 publications

(4 citation statements)

References 33 publications

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“…Previous research reported that the inhibition provided by EGTA was almost reversed by exogenous CaCl 2 . 25 An addition of 3À9 mmol/L CaCl 2 stimulated the enzyme activity (Figure 3C), indicating that there are calcium binding sites in DAO of germinating fava bean.…”

Section: ' Results and Discussionmentioning

confidence: 87%

“…A paper reported that DAO activity of chickpea seedlings was higher when it germinated at 25 °C, and the enzyme activity in the cotyledons and the embryonic axis was sharply inhibited when germinated at higher temperatures (30À35 °C). 25 It suggests that the DAO in different plants has different optimum germination temperatures. The reason may be related to the size of the seeds that are germinated.…”

Section: ' Results and Discussionmentioning

confidence: 99%

“…In this study, it was observed that the optimum germination temperature for increasing DAO activity of fava bean was 30 °C. A paper reported that DAO activity of chickpea seedlings was higher when it germinated at 25 °C, and the enzyme activity in the cotyledons and the embryonic axis was sharply inhibited when germinated at higher temperatures (30–35 °C) . It suggests that the DAO in different plants has different optimum germination temperatures.…”

Section: Resultsmentioning

confidence: 99%

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Factors Influencing Diamine Oxidase Activity and γ-Aminobutyric Acid Content of Fava Bean (Vicia faba L.) during Germination

Yang

Chen

2011

J. Agric. Food Chem.

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Factors (germination time, spectra, temperature, pH, and chemical inhibitors) influencing diamine oxidase (DAO, EC 1.4.3.6) activity and γ-aminobutyric acid (GABA) content of fava bean (Vicia faba L.) during germination were investigated in this study. DAO activity significantly increased in germinating seeds but varied with different organs. The enzyme activity was higher in shoot than that in cotyledon, hypocotyl, and radicle. When seeds were germinated in the dark, DAO activity was 2.35-, 2.00-, 2.36-, 4.40-, and 1.67-fold of that under white, red, blue, green, and yellow spectra, respectively. The optimum germination temperature and pH value for increasing DAO activity were 30 °C and 3.0, respectively. The DAO activity was inhibited significantly by aminoguanidine and sodium ethylenediamine tetracetate, while it was activated by CuCl(2) and CaCl(2). Germinating at an appropriate temperature and pH, 30% of GABA formation was supplied by DAO. Calcium was related to the regulation of DAO activity and GABA accumulation.

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Section: ' Results and Discussionmentioning

confidence: 87%

Section: ' Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Factors Influencing Diamine Oxidase Activity and γ-Aminobutyric Acid Content of Fava Bean (Vicia faba L.) during Germination

Yang

Chen

2011

J. Agric. Food Chem.

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“…Adequate environmental conditions are necessary to ensure the proper development of seedlings. To obtain the most active DAO possible, the germination conditions, including temperature, water, and light, must be optimized to enhance growth of seedlings throughout germination [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Diamine Oxidase as a Therapeutic Enzyme: Study of Germination from Vegetal Sources and Investigation of the Presence of β-N-Oxalyl-L-α,β-diaminopropionic Acid (β-ODAP) Using LC-MS/MS

Boulfekhar

Ohlund

Kumaresan

et al. 2023

IJMS

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Vegetal diamine oxidase (vDAO), an enzyme proposed to relieve symptoms of histaminosis, shows better reactivity with histamine and aliphatic diamines, as well as higher enzymatic activity than DAO of animal origin. The objective of this study was to evaluate the enzyme activity of vDAO from germinating grains from Lathyrus sativus (grass pea) and Pisum sativum (pea), and to verify the presence of a neurotoxin, β-N-Oxalyl-L-α,β-diaminopropionic acid (β-ODAP), in the crude extract obtained from their seedlings. A targeted liquid chromatography–multiple-reaction monitoring mass spectrometry method was developed and used to quantify β-ODAP in the analysed extracts. An optimized sample preparation procedure, involving protein precipitation with acetonitrile followed by mixed-anion exchange solid-phase extraction, allowed for high sensitivity and good peak shape for β-ODAP detection. The Lathyrus sativus extract exhibited the highest vDAO enzyme activity of the extracts, followed by the extract from pea cultivar Amarillo from the Crop Development Centre (CDC). The results have also shown that even though β-ODAP was present in the crude extract from L. sativus, its content was far below the toxicity threshold (300 mg of β-ODAP/kg body/day). CDC Amarillo showed 5000-fold less β-ODAP than the undialysed L. sativus extract. It was concluded that both species can be considered as convenient sources of vDAO for potential therapeutic use.

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Polyamine contents, ethylene synthesis, and BrACO2 expression during turnip germination

Puga¹,

Gallardo²,

Rodríguez-Gacio³

et al. 2006

Biologia plant.

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Contents of total free [PA(S)] and conjugated polyamines [PA(SH), PA(PH)] were higher in turnip (Brassica rapa L. cv. Rapa) seeds during imbibition (0 -36 h) and radicle protrusion (36 -48 h) than during the further growth (10 d).Ethylene production was activated with the protrusion, reaching a maximum at the second day of germination and dropping afterwards. The application of ethrel accelerated radicle emergence but the direct intervention of ethylene in the breaking of the seed coat was not clear from the use of ethylene-biosynthesis inhibitors (CoCl 2 and AVG). Finally, in this work the gene BrACO2 was characterized. Although its expression was not detected in seeds through zygotic embryogenesis, it increased concomitantly with the germination process.

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Diamine Oxidase Activity During the Germinative and Post-Germinative Growth of the Embryonic Axis in Chickpea Seeds

Cited by 3 publications

References 33 publications

Factors Influencing Diamine Oxidase Activity and γ-Aminobutyric Acid Content of Fava Bean (Vicia faba L.) during Germination

Factors Influencing Diamine Oxidase Activity and γ-Aminobutyric Acid Content of Fava Bean (Vicia faba L.) during Germination

Diamine Oxidase as a Therapeutic Enzyme: Study of Germination from Vegetal Sources and Investigation of the Presence of β-N-Oxalyl-L-α,β-diaminopropionic Acid (β-ODAP) Using LC-MS/MS

Polyamine contents, ethylene synthesis, and BrACO2 expression during turnip germination

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