Diameter of titanium nanotubes influences anti-bacterial efficacy

Ercan, Batur; Taylor, Erik N.; Alpaslan, Ece; Webster, Thomas J.

doi:10.1088/0957-4484/22/29/295102

Cited by 211 publications

(187 citation statements)

References 46 publications

(59 reference statements)

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“…Therefore, controlled anodized Ti nanotube diameter size formation is important toward promising amounts of antibacterial performances, which can be applied to improved antimicrobial behavior and tissue growth properties. 118 Aside from diameters, under a solar-simulated light, double-doped TiO 2 nanoparticles (TiO 2 NPs) have acted as a visible lightactive antibacterial agent. Using sol-gel method, doubledoped TiO 2 NPs exhibited the most antibacterial activity in comparison to single-doped TiO 2 NPs under visible light irradiation.…”

Section: Synthesismentioning

confidence: 99%

Antibacterial properties and toxicity from metallic nanomaterials

Vimbela¹,

Ngo²,

Fraze³

et al. 2017

IJN

497

334

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The era of antibiotic resistance is a cause of increasing concern as bacteria continue to develop adaptive countermeasures against current antibiotics at an alarming rate. In recent years, studies have reported nanoparticles as a promising alternative to antibacterial reagents because of their exhibited antibacterial activity in several biomedical applications, including drug and gene delivery, tissue engineering, and imaging. Moreover, nanomaterial research has led to reports of a possible relationship between the morphological characteristics of a nanomaterial and the magnitude of its delivered toxicity. However, conventional synthesis of nanoparticles requires harsh chemicals and costly energy consumption. Additionally, the exact relationship between toxicity and morphology of nanomaterials has not been well established. Here, we review the recent advancements in synthesis techniques for silver, gold, copper, titanium, zinc oxide, and magnesium oxide nanomaterials and composites, with a focus on the toxicity exhibited by nanomaterials of multidimensions. This article highlights the benefits of selecting each material or metal-based composite for certain applications while also addressing possible setbacks and the toxic effects of the nanomaterials on the environment.

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Section: Synthesismentioning

confidence: 99%

Antibacterial properties and toxicity from metallic nanomaterials

Vimbela¹,

Ngo²,

Fraze³

et al. 2017

IJN

497

334

View full text Add to dashboard Cite

show abstract

“…This is in accordance with previous observations of reduced biofilm formation and bacterial adhesion on nanostructured substrates. 17,25,26 For a given surface chemistry, the degree of hydrophilicity will be influenced by nanostructures. On a hydrophilic material, the presence of nanostructures will lead to increased hydrophilicity.…”

Section: Surface Nanotopography Influences Bacterial Adhesion and Biomentioning

confidence: 99%

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

Svensson¹,

Forsberg²,

Hulander³

et al. 2014

IJN

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The role of material surface properties in the direct interaction with bacteria and the indirect route via host defense cells is not fully understood. Recently, it was suggested that nanostructured implant surfaces possess antimicrobial properties. In the current study, the adhesion and biofilm formation of Staphylococcus epidermidis and human monocyte adhesion and activation were studied separately and in coculture in different in vitro models using smooth gold and well-defined nanostructured gold surfaces. Two polystyrene surfaces were used as controls in the monocyte experiments. Fluorescent viability staining demonstrated a reduction in the viability of S. epidermidis close to the nanostructured gold surface, whereas the smooth gold correlated with more live biofilm. The results were supported by scanning electron microscopy observations, showing higher biofilm tower formations and more mature biofilms on smooth gold compared with nanostructured gold. Unstimulated monocytes on the different substrates demonstrated low activation, reduced gene expression of pro- and anti-inflammatory cytokines, and low cytokine secretion. In contrast, stimulation with opsonized zymosan or opsonized live S. epidermidis for 1 hour significantly increased the production of reactive oxygen species, the gene expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10, as well as the secretion of TNF-α, demonstrating the ability of the cells to elicit a response and actively phagocytose prey. In addition, cells cultured on the smooth gold and the nanostructured gold displayed a different adhesion pattern and a more rapid oxidative burst than those cultured on polystyrene upon stimulation. We conclude that S. epidermidis decreased its viability initially when adhering to nanostructured surfaces compared with smooth gold surfaces, especially in the bacterial cell layers closest to the surface. In contrast, material surface properties neither strongly promoted nor attenuated the activity of monocytes when exposed to zymosan particles or S. epidermidis .

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“…Ercan et al 42 investigated the antibacterial efficacy of Ti nanotubes with diameters of 20, 40, 60, 80 nm. They demonstrated that nanotubular structures (except those of 20 nm in diameter) could reduce the number of live bacteria adhering to the surface, and for larger diameters (40-80 nm), fewer living bacteria were observed on the surfaces.…”

Section: Figurementioning

confidence: 99%

Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters

Lin¹,

Tan²,

Duan³

et al. 2014

IJN

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Titania nanotubes loaded with antibiotics can deliver a high concentration of antibiotics locally at a specific site, thereby providing a promising strategy to prevent implant-associated infections. In this study we have fabricated titania nanotubes with various diameters (80, 120, 160, and 200 nm) and 200 nm length via electrochemical anodization. These nanotubes were loaded with 2 mg of gentamicin using a lyophilization method and vacuum drying. A standard strain, Staphylococcus epidermidis (American Type Culture Collection 35984), and two clinical isolates, S. aureus 376 and S. epidermidis 389, were selected to investigate the anti-infective ability of the gentamicin-loaded nanotubes (NT-G). Flat titanium (FlatTi) and nanotubes with no drug loading (NT) were also investigated and compared. We found that NT-G could significantly inhibit bacterial adhesion and biofilm formation compared to FlatTi or NT, and the NT-G with 160 nm and 200 nm diameters had stronger antibacterial activity because of the extended drug release time of NT-G with larger diameters. The NT also exhibited greater antibacterial ability than the FlatTi, while nanotubes with 80 nm or 120 nm diameters had better effects. Furthermore, human marrow derived mesenchymal stem cells were used to evaluate the effect of nanotubular topographies on the osteogenic differentiation of mesenchymal stem cells. Our results showed that NT-G and NT, especially those with 80 nm diameters, significantly promoted cell attachment, proliferation, spreading, and osteogenic differentiation when compared to FlatTi, and there was no significant difference between NT-G and NT with the same diameter. Therefore, nanotube modification and gentamicin loading can significantly improve the antibacterial ability and osteogenic activity of orthopedic implants.

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Diameter of titanium nanotubes influences anti-bacterial efficacy

Cited by 211 publications

References 46 publications

Antibacterial properties and toxicity from metallic nanomaterials

Antibacterial properties and toxicity from metallic nanomaterials

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters

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