Diamagnetic repulsion of particles for multilaminar flow assays

Tarn, Mark D.; Elders, Luke T.; Peyman, Sally A.; Pamme, Nicole

doi:10.1039/c5ra21867e

Cited by 6 publications

(5 citation statements)

References 78 publications

(108 reference statements)

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“…Tarn et al [164] demonstrated a proof-of-principle multi-laminar flow assay on the surface of diamagnetic particles (Figure 15A, top). Streptavidin-functionalized polymer beads were suspended in a paramagnetic MnCl 2 solution that was introduced into a flow-focusing region of the microchip (Figure 15A, bottom).…”

Section: Particle Medium Exchangementioning

confidence: 99%

“…( A ) Schematic of the device and setup used for the diamagnetic repulsion-based streptavidin-biotin assay (top) and images showing the deflection of a polymer bead across multiple streams (bottom). Adapted with permission from Tarn et al [164], © 2015 The Royal Society of Chemistry. ( B ) Schematic illustration of a biocompatible cell separation in a multiphase ferrofluid flow (left) and image of the separation/washing process of spiked cancer cells from WBCs at the end of the straight channel section (right).…”

Section: Figurementioning

confidence: 99%

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Recent Advances in Continuous-Flow Particle Manipulations Using Magnetic Fluids

Xuan¹

2019

Micromachines

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Magnetic field-induced particle manipulation is simple and economic as compared to other techniques (e.g., electric, acoustic, and optical) for lab-on-a-chip applications. However, traditional magnetic controls require the particles to be manipulated being magnetizable, which renders it necessary to magnetically label particles that are almost exclusively diamagnetic in nature. In the past decade, magnetic fluids including paramagnetic solutions and ferrofluids have been increasingly used in microfluidic devices to implement label-free manipulations of various types of particles (both synthetic and biological). We review herein the recent advances in this field with focus upon the continuous-flow particle manipulations. Specifically, we review the reported studies on the negative magnetophoresis-induced deflection, focusing, enrichment, separation, and medium exchange of diamagnetic particles in the continuous flow of magnetic fluids through microchannels.

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Section: Particle Medium Exchangementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Recent Advances in Continuous-Flow Particle Manipulations Using Magnetic Fluids

Xuan¹

2019

Micromachines

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“…Tarn et al recently demonstrated diamagnetic repulsion of particles in paramagnetic solutions and ferrofluids. 21 Exploiting the weak diamagnetism of most biological cells and polymer microparticles, they achieved fluorescent biotin coating and repulsion of polymer microparticles in a paramagnetic washing solution. Even though they used non-ferromagnetic particles, they still needed special washing media to generate sufficient repulsion of the intrinsically diamagnetic particles.…”

Section: Introductionmentioning

confidence: 99%

Acoustofluidic coating of particles and cells

et al. 2016

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On-chip microparticle and cell coating technologies enable a myriad of applications in chemistry, engineering, and medicine. Current microfluidic coating technologies often rely on magnetic labeling and concurrent deflection of particles across laminar streams of chemicals. Herein, we introduce an acoustofluidic approach for microparticle and cell coating by implementing tilted-angle standing surface acoustic waves (taSSAWs) into microchannels with multiple inlets. The primary acoustic radiation force generated by the taSSAW field was exploited in order to migrate the particles across the microchannel through multiple laminar streams which contained the buffer and coating chemicals. We demonstrate effective coating of polystyrene microparticles and HeLa cells without the need for magnetic labelling. We characterized the coated particles and HeLa cells with fluorescence microscopy and scanning electron microscopy. Our acoustofluidicbased particle and cell coating method is label-free, biocompatible, and simple. It can be useful in the on-chip manufacturing of many functional particles and cells.

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“…The former motion requires particles to be manipulated being magnetizable. , However, the majority of biological (the only exceptions being red blood cells and magnetotactic bacteria) and synthetic (except magnetic particles) particles are diamagnetic, which must be tagged with one or multiple magnetic particles in order to experience positive magnetophoresis under a nonuniform magnetic field. Therefore, there has been growing interest in using magnetic fluids, which include paramagnetic solutions and ferrofluids, to induce negative magnetophoresis for continuous-flow deflection, − concentration, − and separation − of various diamagnetic particles (see the recent review articles from Yang et al and Zhao et al as well as the references cited therein).…”

mentioning

confidence: 99%

Tunable, Sheathless Focusing of Diamagnetic Particles in Ferrofluid Microflows with a Single Set of Overhead Permanent Magnets

Chen

Malekanfard

et al. 2018

Anal. Chem.

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There has been increasing interest in the use of magnetic fluids to manipulate diamagnetic particles in microfluidic devices. Current methods for diamagnetic-particle focusing in magnetic fluids require either a pair of repulsive magnets or a diamagnetic sheath flow. We demonstrate herein a tunable, sheathless focusing of diamagnetic particles in a microchannel ferrofluid flow with a single set of overhead permanent magnets. Particles are focused into a single stream near the bottom wall of a straight rectangular microchannel, where a magnetic-field minimum is formed as a result of the magnetization of the ferrofluid. This focusing can be readily switched off and on by removing and replacing the permanent magnets. More importantly, the particle-focusing position can be tuned by shifting the magnets with respect to the microchannel. We perform a systematic experimental study of the parametric effects of the fluid-particle-channel system on diamagnetic-particle focusing in terms of a defined particle-focusing effectiveness.

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Diamagnetic repulsion of particles for multilaminar flow assays

Abstract: A continuous multilaminar flow reaction was performed on functionalised polymer particlesviadiamagnetic repulsion forces, using a simple, inexpensive setup.

Cited by 6 publications

References 78 publications

Recent Advances in Continuous-Flow Particle Manipulations Using Magnetic Fluids

Recent Advances in Continuous-Flow Particle Manipulations Using Magnetic Fluids

Acoustofluidic coating of particles and cells

Tunable, Sheathless Focusing of Diamagnetic Particles in Ferrofluid Microflows with a Single Set of Overhead Permanent Magnets

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