Dialyzable leukocyte extract used in the prevention of Salmonella infection in calves

Mikula, I.; Pistl, J.; Rosocha, Ján

doi:10.1016/0165-2427(92)90073-y

Cited by 5 publications

(2 citation statements)

References 11 publications

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“…The first successful studies on the protective effect of TF against Salmonella typhimurium were performed on mouse model [17,77]. Later, Mikula et al [78] and Mikula et al [2] reported bacteriological, immunological and clinical trials of specific and non-specific DLE on calves which have been experimentally infected with a virulent strain Salmonella typhimurium. Injecting the calves intravenously with specific DLE protected them against the experimental infection with a pathogenic strain S. typhimurium 4/5.…”

Section: Animal Infectious and Parasitic Diseasesmentioning

confidence: 99%

Immunotherapy with Dialyzable Leukocyte Extracts Containing Transfer Factor

Arnaudov¹

2017

Immunotherapy - Myths, Reality, Ideas, Future

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Dialyzable leukocyte extracts (DLE) are complexes consisting of a large number of low molecular weight substances. These extracts possess immunomodulatory properties, which are mainly attributed to small peptides with molecular weight of 3.5-6.0 kDa called "transfer factor." This chapter reviews the nature and immunological characteristics of DLE containing transfer factor (TF), their mechanism of action and the possible uses as immunomodulators in human and veterinary medicine. A main advantage of TF-preparations as immunotherapeutic agents is that they induce a rapid immune response against the pathogen (within 24 h) and thereby reduce the time for the patient immune response by 9-13 days. The low level of difficulty of the process of obtaining protocols determines their relatively low cost and the possibility to combine them with other therapeutic agents during treatment makes them subject to medical applications in the future, including against some new diseases.

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Section: Animal Infectious and Parasitic Diseasesmentioning

confidence: 99%

Immunotherapy with Dialyzable Leukocyte Extracts Containing Transfer Factor

Arnaudov¹

2017

Immunotherapy - Myths, Reality, Ideas, Future

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“…In 1982, Smith et al [73] performed the first successful studies on the protective effect of the murine TF against Salmonella typhimurium using the mouse model. Later, Mikula et al [74] and Mikula et al [2] reported bacteriological, immunological and clinical trials of specific and non-specific DLE on calves experimentally infected with a virulent strain S. typhimurium 4/5. Injecting the calves intravenously with specific DLE protected them against the experimental infection.…”

Section: Using Dle Preparations In Veterinary Medicinementioning

confidence: 99%

Dialysable leukocyte extracts in immunotherapy

Arnaudov

Kostova

2015

Biotechnology & Biotechnological Equipment

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Dialysable leukocyte extracts (DLE) are complexes consisting of a large number of low-molecular-weight substances. These extracts have immunomodulatory properties, which are mainly attributed to small peptides commonly referred to as 'transfer factor'. This review focuses on the characteristics of DLE with transfer factor activity, together with the methods for their preparation and purification. The opportunities for applying the extracts for immunotherapy purposes against various diseases in humans and domestic animals are also discussed. Their ease of preparation and relatively low cost combined with the rapid positive effect they produce will make DLE subject to application in medicine in the future, including their use against new nosological entities.

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Some properties and protective activity of specific DLE against Salmonella cholerae suis infection

Arnaudov¹,

Tziporkov

1996

Biotherapy

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From a rabbit lymphoid tissue, twice immunized with a Salmonella ch. suis vaccine, was obtained a dialysable leucocyte extract (DLE) (m. w. 10,000Da; protein content 1.14 mg/ml; content of ribose 2.7 mg/ml; A260/A280 ratio 2.17 and pH 6.8). By gel filtration on Sephadex G-25, six peaks were obtained and activity was found in peak IV. The activity of the extract was determined by a dermo-application test (DAT) on 10 cows. The protective effect was tested by a challenge with Salmonella ch. suis and Salmonella dublin pathogen strains on white mice intraperitoneally treated with DLE. The DAT proved to be positive in 8 of the 10 cows. When applied on white mice, it induced a high specific protective effect against Salmonella ch. suis (70%), but not against Salmonella dublin infection.

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Dialyzable leukocyte extract used in the prevention of Salmonella infection in calves

Cited by 5 publications

References 11 publications

Immunotherapy with Dialyzable Leukocyte Extracts Containing Transfer Factor

Immunotherapy with Dialyzable Leukocyte Extracts Containing Transfer Factor

Dialysable leukocyte extracts in immunotherapy

Some properties and protective activity of specific DLE against Salmonella cholerae suis infection

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