Diallyl trisulfide induces apoptosis in Jurkat cells by the modification of cysteine residues in thioredoxin

Watanabe, Kei; Hosono, Takashi; Watanabe, Kazuhiro; Hosono-Fukao, Tomomi; Ariga, Toshio; Seki, Taiichiro

doi:10.1080/09168451.2014.921564

Cited by 8 publications

(7 citation statements)

References 11 publications

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“…Ca 2+ can also activate endonuclease and degrade nuclear DNA. On the other hand, Ca 2+ overload within the cytoplasm may cause mitochondrial swelling, outer membrane rupture (Redza-Dutordoir and Averill-Bates 2016; Ha et al 2019), and even fragmentation under special conditions, which in turn can induce cellular apoptosis (Li et al 2015;Watanabe et al 2014). In the present study, intracellular ROS and cytoplasmic Ca 2+ in the PM 2.5 exposed cells significantly increased (Fig.…”

Section: Discussionsupporting

confidence: 46%

Cytotoxicity induced by fine particulate matter (PM2.5) via mitochondria-mediated apoptosis pathway in rat alveolar macrophages

Wei

Yuan

et al. 2021

Environ Sci Pollut Res

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Although positive associations exist between ambient particulate matter (PM 2.5 ; diameter ≤ 2.5 μm) and the morbidity and mortality rates for respiratory diseases, the biological mechanisms of the reported health effects are unclear. Considering that alveolar macrophages (AM) are the main cells responsible for phagocytic clearance of xenobiotic particles that reach the airspaces of the lungs, the purpose of this study was to investigate whether PM 2.5 induced AM apoptosis, and investigate its possible mechanisms. Freshly isolated AM from Wistar rats were treated with extracted PM 2.5 at concentrations of 33, 100, or 300 μg/mL for 4 h; thereafter, the cytotoxic effects were evaluated. The results demonstrated that PM 2.5 induced cytotoxicity by decreasing cell viability and increasing lactate dehydrogenase (LDH) levels in AMs. The levels of reactive oxygen species (ROS) and intracellular calcium cations (Ca 2+) markedly increased in higher PM 2.5 concentration groups. Additionally, the apoptotic ratio increased, and the apoptosis-related proteins BCL2-associated X (Bax), caspase-3, and caspase-9 were upregulated, whereas B cell lymphoma-2 (Bcl-2) protein levels were downregulated following PM 2.5 exposure. Cumulative findings showed that PM 2.5 induced apoptosis in AMs through a mitochondrial-mediated pathway, which indicated that PM 2.5 plays a significant role in lung injury diseases.

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Section: Discussionsupporting

confidence: 46%

Cytotoxicity induced by fine particulate matter (PM2.5) via mitochondria-mediated apoptosis pathway in rat alveolar macrophages

Wei

Yuan

et al. 2021

Environ Sci Pollut Res

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“…We previously showed that DATS inhibited cell cycle progression in cancer cells. [3,7,9] Marquez et al reported that cell proliferation regulates adipogenesis in human adipose tissue-derived mesenchymal stem cells. [43] Thus, the causal relationship between DATS-mediated cell cycle alteration and adipogenesis remains unclear, although DATS may influence adipogenesis through cell cycle regulation in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…[2] We have previously reported on a range of positive health benefits of DATS, including hepato-protective, anticancer, and antithrombotic effects. [3][4][5][6][7][8][9] Obesity is a serious health problem; 39% of the world's adult population is overweight and 13% is obese. These proportions have been increasing year by year.…”

Section: Introductionmentioning

confidence: 99%

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Diallyl Trisulfide Prevents Obesity and Decreases miRNA‐335 Expression in Adipose Tissue in a Diet‐Induced Obesity Rat Model

Miura

Ikeda

Abe

et al. 2021

Molecular Nutrition Food Res

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Scope: Diallyl trisulfide (DATS), an organosulfur compound generates in crushed garlic, has various beneficial health effects. A growing body of evidence indicates that miRNAs are involved in the pathology of lifestyle diseases including obesity. The anti-obesogenic effect of garlic is previously reported; however, the effects of DATS on obesity, and the relationship between garlic compounds and the involvement of miRNA remains unclear. Here, the anti-obesogenic activity of DATS and the potential role of miRNA in a diet-induced obesity rat model are investigated. Methods and Results: Oral administration of DATS suppressed body and white adipose tissue (WAT) weight gain in rats fed a high-fat diet compared with vehicle-administered rats. DATS lowered the plasma and liver triglyceride levels in obese rats, and decreased lipogenic mRNA levels including those of Srebp1c, Fasn, and Scd1 in the liver. DATS also suppressed de novo lipogenesis in the liver. Transcriptomic analyses of miRNA and mRNA in the epididymal WAT of obese rats using microarrays revealed that DATS decreased miRNA-335 expression and normalized the obesity-related mRNA transcriptomic signatures in epididymal WAT. Conclusion: The potent anti-obesogenic effects of DATS and its possible mechanism of action was clearly demonstrated in this study.

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“…As a lipophilic compound extracted from garlic, DATS has been shown to be a novel anticancer agent. Numerous studies have indicated that DATS has strong anti-proliferative and pro-apoptotic activities in many cell lines (51)(52)(53). DATS-rich garlic oil benefits blood anti-coagulation factors and further prevents the development of thrombus.…”

Section: Discussionmentioning

confidence: 99%

Suppressive role of diallyl trisulfide in the activated platelet-mediated hematogenous metastasis of MDA-MB-231 human breast cancer cells

Yu-ping

Zhao

Wang

et al. 2017

International Journal of Molecular Medicine

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Accumulating evidence has indicated that garlic consumption may reduce the risk of developing several types of cancer, and extensive studies have revealed the effects of its bioactive component, diallyl trisulﬁde (DATS), on the proliferation and apoptosis of tumor cells. The present study was undertaken to examine whether DATS affects hematogenous metastasis. In view of the dynamic crosstalk interplayed by tumor cells and platelets in hematogenous metastasis, we attempted to demonstrate the role of DATS in the metastatic behavior of MDA-MB-231 human breast cancer cells, which were co-incubated with activated platelets. Indeed, our data indicated that DATS significantly blocked platelet activation and aggregation induced by platelet-activating factor (PAF), and decreased the production of thromboxane B2 (TXB2). It was also found that DATS suppressed the migration and invasion of MDA-MB-231 cells in the presence of platelets activated by PAF in vitro in a dose-dependent manner. Furthermore, our results revealed thaat the release of activated TGF-β1 in the platelet-tumor cell system was markedly attenuated by DATS. Therefore, our findings strongly suggest that the diverse pharmacological activities of DATS are at least partially reflected by the interruption of the activated platelets-mediated metastasis of breast cancer cells.

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Diallyl trisulfide induces apoptosis in Jurkat cells by the modification of cysteine residues in thioredoxin

Cited by 8 publications

References 11 publications

Cytotoxicity induced by fine particulate matter (PM2.5) via mitochondria-mediated apoptosis pathway in rat alveolar macrophages

Cytotoxicity induced by fine particulate matter (PM2.5) via mitochondria-mediated apoptosis pathway in rat alveolar macrophages

Diallyl Trisulfide Prevents Obesity and Decreases miRNA‐335 Expression in Adipose Tissue in a Diet‐Induced Obesity Rat Model

Suppressive role of diallyl trisulfide in the activated platelet-mediated hematogenous metastasis of MDA-MB-231 human breast cancer cells

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