Diallyl disulfide inhibits the metastasis of type Ⅱ esophageal‑gastric junction adenocarcinoma cells via NF-κB and PI3K/AKT signaling pathways in vitro

Abstract

Esophageal-gastric junction adenocarcinoma (AEG) is an aggressive tumor with high incidence and dismal prognosis worldwide. Despite significant advances in therapeutic strategies, the 5-year survival rate still remains low. Diallyl disulfide (DADS), which is one of the major volatile components isolated from garlic, has been shown to have multi-targeted antitumor activities in a variety of cancer cells. However, the exact anti-metastatic effects and underlying molecular mechanisms of DADS in AEG have not been elucidated. The present study demonstrated that DADS inhibited cell viability of OE19 cells with low cytotoxicity to healthy hepatocytes, L02 cells, in vitro. Non-toxic doses of DADS were ≤10 µg/ml for a 24-h treatment. Our data showed that these non-toxic doses of DADS were found to block the metastasis of OE19 cells by suppressing MMPs, increasing u-PA and TIMPs, as well as altering the balance of MMPs/TIMPs, which at least in part resulted from the suppression of NF-κB and PI3K/AKT signaling pathways. The present study provides a previously neglected insight into the investigation of DADS in suppressing tumor metastasis and its underlying molecular mechanisms in vitro. Hence, DADS could be a promising anticancer agent for anti-metastatic treatment of AEG in the future.

PRR11 Overexpression Facilitates Ovarian Carcinoma Cell Proliferation, Migration, and Invasion Through Activation of the PI3K/AKT/β-Catenin Pathway

“…ECM degradation is positively mediated by MMP/TIMP expression, as alterations in the balance of MMP/TIMP expression lead to the invasion and migration of OC cells [19,20]. Here, we observed that PRR11 silencing mitigated the invasion and migration of siRNA-PRR11 HO-8910 cells, which was associated with reduced levels of MMP2, but increased levels of TIMP-2 in OC.…”

Comparative study on the composition of four different varieties of garlic

“…These results suggest that under acidic conditions (pH 3.0), ‘Ershuizao’ and ‘Hongqixing’ garlic easily react and convert sucrose to 5-hydroxymethylfurfural, which has antitumor effects. However, previous studies on the antitumor effects of garlic focused on other organosulphur compounds, such as diallyl disulphide (DADS) (Liao et al, 2009; Yin et al, 2018), DATS (Li & Lu, 2002), and ajoene (Li et al, 2002).…”

Peer Review #1 of "Comparative study on the composition of four different varieties of garlic (v0.1)"

“…These results suggest that under acidic conditions (pH 3.0), 'Ershuizao' and 'Hongqixing' garlic easily react and convert sucrose to 5-Hydroxymethylfurfural, which has antitumor effects. However, previous studies on the antitumor effects of garlic focused on other organosulfur compounds, such as diallyl disulfide (DADS) (Liao et al 2009;Yin et al 2018), DATS (Li & Lu 2002), and ajoene .…”