Diallel Cross Application and Histomolecular Characterization: An Attempt to Develop Reference Stock of Labeo ariza

Abstract

The objective of the present study was to evaluate the growth performance and genetic variation in diallel crosses of Ariza labeo (Labeo ariza) originating from three geographically separated rivers (Atrai, Jamuna and Kangsha) in Bangladesh. Intra (G1K♀K♂, G2J♀J♂, and G3A♀A♂) and inter (G4K♀A♂, G5K♀J♂, G6A♀K♂, G7A♀J♂, G8J♀K♂, and G9J♀A♂) stocks were produced following diallel cross (sex ratio—1:1 and n = 48; 16 from each river). Reproductive and growth performance, muscle cellularity and genetic variation following genotyping of eight microsatellite markers (Lr1, Lr2, Lr3, Lr22, Lr24, Lr27, Lr28 and Lr29) and analysis of all crossbreeds was performed. The fertilization (95% ± 2.11%), hatching (88% ± 1.03%), and survival rates (82% ± 1.88%) of G4K♀A♂ were higher compared to other groups. With respect to length and weight gains (2.67 ± 0.4 cm and 3.39 ± 0.2 g), SGR (3.23% ± 0.20%), and heterosis (8.87% and 24.74%) G4K♀A♂ was the superior group. A higher number of hyperplastic muscle fibers, mean number of alleles (2.75) and mean observed heterozygosity (0.417) from G4K♀A♂ could be interpreted to mean that G4K♀A♂ comprise better performance efficiency compared to others and are considered for continuing the L. ariza stock improvement program.

Assessment of genetic diversity among wild and captive-bred Labeo rohita through microsatellite markers and mitochondrial DNA

“…It has high aquaculture and commercial importance worldwide, especially in Asia, and occupies the topmost rank among Indian major carp where it is consumed owing to its fame for being a tasty fish which results in its high market value (Rahman et al, 2005). However, over the past two decades, many investigators from Bangladesh, India, and Pakistan reported a decline in the genetic diversity of wild and captive stocks of L. rohita (Ahammad et al, 2022;Khan, 2008;Qadeer & Abbas, 2017). Therefore, the current study was designed to evaluate the genetic variation in both wild and captive populations of the indigenous culturable species L. rohita, by utilizing microsatellite markers and mitochondrial marker (CO1).…”