Diallel analyses of soluble sugar content in cowpea ( Vigna unguiculata L. Walp.)

Okra pod is sensitive to low temperature, which results in chilling injury under improper low‐temperature storage. This study aimed to evaluate the effect of different concentrations of methyl jasmonate (MeJA) treatment on okra pod stored at 4 ± 1°C for 12 days and illuminate the mechanism of MeJA alleviating chilling injury. Compared to the control, MeJA treatments maintained lower relative electric conductivity (REC), chilling injury (CI) degree, and lignin content, as well as higher total soluble solids, total soluble sugar, pectin content, and chlorophyll content. The factor analysis was applied to comprehensively evaluate the effects of MeJA so that 1 μmol/L MeJA was screened as the optimum concentration to maintain the okra quality throughout the storage time. In contrast with control, MeJA not only accelerated the generation of antioxidant substances (phenolics and flavonoids) but also increased the superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD) activity, inhibited malondialdehyde (MDA), hydrogen peroxide (H2O2) content accumulation, and the polyphenol oxidase (PPO) activity. This work confirmed that MeJA could effectively alleviate chilling injury and maintain the quality during cold‐stored by regulating reactive oxygen species (ROS) metabolism. These results provide theoretical guidance for the application of MeJA in okra storage and preservation.

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“…Total soluble sugars content was assessed by the method of Maina et al ( 2017 ) The results were expressed as OD 540 /g.…”

Section: Methodsmentioning

confidence: 99%

Methyl jasmonate treatment alleviates chilling injury and improves antioxidant system of okra pod during cold storage

Liu

Chen

et al. 2023

Food Science & Nutrition

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“…The contents of total sugar and reducing sugar were detected by 3, 5-dinitrosalicylic acid colourimetry (Nassourou et al, 2017). The content of crude fiber was determined using the apparatus Hanon F800 Fiber Analyzer (YiKe Technology, Chen Du, China).…”

Section: Determination Of Biochemical Indices In Tbsmentioning

confidence: 99%

Comparative study of the effects of Tartary buckwheat seed and sprout consumption on the physiological indices and gut microbiota of C57BL/6J mice

Nan¹,

Zhao²,

Wu³

et al. 2024

Food Science and Human Wellness

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Tartary buckwheat (Fagopyrum Tataricum) is a well-known pseudocereal for its health and economic value. However, abundant antinutritional factors (ANFs) reduces its health benefits. As reported, germination can improve the nutritional profile of grains. In this study, we systematically evaluate the safety of Tartary buckwheat seeds (TB) and Tartary buckwheat sprouts (TBS) used as high active ingredients. After evaluating nutrition levels, bioactive compounds and ANFs in TBS during germinating, fifth-day TBS were selected as the raw material. C57BL/6J mice were gavaged daily with distilled water, TB, or TBS for 6 weeks. The physiological indices related to ANFs were determined. Results showed that the TB intake tends to generate negative effects on the gut microbiota, and organs. Additionally, upon TB intake, the Fe 3+ content in serum, trypsin activity in pancreas and jejunum decreased, while the cytokine, IgE, and histamine levels in serum, water content in faeces, cytokine levels in liver and jejunum increased. Conversely, TBS did not induce any obvious negative effects on the above relevant indices and showed better lipid-lowering effect. Altogether, TBS are safer and more effective as a raw material to produce the functional food for long-term consumption with the intention of preventing and treating hyperlipidaemia.

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“…However, this seed soluble sugar content characteristic has not received much attention in the cowpea breeding program, which has been primarily concerned with increasing yield, pest resistance, draught tolerance, protein content and other quality characteristics [3] Nassourou et al (2017) assessed the amount of total soluble sugar among 15 fully homozygous cowpea cultivars using the phenol sulfuric acid reagent method [3]. The objective of the present work was to evaluate the soluble sugar content in cowpea seeds and investigate elite germplasm with desired soluble sugar contents in order to propose a suitable breeding strategy for improving sweetness of cowpea seeds.…”

Section: Introductionmentioning

confidence: 99%

Screening of Seed Soluble Sugar Content in Cowpea (<i>Vigna unguiculata</i> (L.) Walp)

Wang¹,

Ravelombola²,

Yang³

et al. 2018

AJPS

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Cowpea (Vigna unguiculata (L.) Walp) is a legume crop grown worldwide to provide protein, starch, soluble sugar, amino acids, fatty acids, minerals, and vitamins for human consumption and animal feed. Soluble sugar is an important physiological trait in cowpea seeds. It not only plays an important role in storability and abiotic stress tolerance in seeds, but also provides energy to the human body and improves cooking quality by giving a desirable taste and mouth feel. Therefore, this research aimed to evaluate variation of cowpea seed soluble sugar content, and assess the soluble sugar content of cowpea varieties with different seed coat colors for cowpea consumers. A total of 113 cowpea genotypes were used, including 89 USDA GRIN germplasm accessions and 24 advanced breeding lines developed from University of Arkansas, AR, USA. Seed soluble sugar content was measured using a Spectrophotometer by phenol-sulphuric acid method. The results showed that the seed soluble sugar contents among 113 cowpea genotypes exhibited a wide range varying from 32.6 mg/g to 86.1 mg/g with an average of 54.5 mg/g. The five highest soluble sugar contents in seeds were found in the Arkansas cultivar Empire (86.1 mg/g), USDA germplasm accession PI583202 (84.5 mg/g), Arkansas advanced breeding line 09-655 (82.1 mg/g), USDA accession PI601085 (81.6 mg/g), and Arkansas advanced breeding line 09-529 (80.9 mg/g), and they can be used in cowpea breeding programs to develop new cowpea cultivars with higher seed soluble sugar content. It was also observed that the soluble sugar contents in Arkansas commercial cultivars and advanced breeding lines were higher than those in USDA germplasm and the seeds with colorful coat may have soluble sugar levels desirable by consumers.

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Diallel analyses of soluble sugar content in cowpea ( Vigna unguiculata L. Walp.)

Cited by 9 publications

References 20 publications

Methyl jasmonate treatment alleviates chilling injury and improves antioxidant system of okra pod during cold storage

Methyl jasmonate treatment alleviates chilling injury and improves antioxidant system of okra pod during cold storage

Comparative study of the effects of Tartary buckwheat seed and sprout consumption on the physiological indices and gut microbiota of C57BL/6J mice

Screening of Seed Soluble Sugar Content in Cowpea (<i>Vigna unguiculata</i> (L.) Walp)

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Diallel analyses of soluble sugar content in cowpea ( Vigna unguiculata L. Walp.)

Cited by 9 publications

References 20 publications

Methyl jasmonate treatment alleviates chilling injury and improves antioxidant system of okra pod during cold storage

Methyl jasmonate treatment alleviates chilling injury and improves antioxidant system of okra pod during cold storage

Comparative study of the effects of Tartary buckwheat seed and sprout consumption on the physiological indices and gut microbiota of C57BL/6J mice

Screening of Seed Soluble Sugar Content in Cowpea (&lt;i&gt;Vigna unguiculata&lt;/i&gt; (L.) Walp)

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Screening of Seed Soluble Sugar Content in Cowpea (<i>Vigna unguiculata</i> (L.) Walp)