Diagnostik, Klassifikation und Prognosefaktoren der akuten myeloischen Leukämie

Heilmeier, Bernhard; Buske, Christian; Spiekermann, Karsten; Bohlander, Stefan K.; Feuring‐Buske, Michaela; Hiddemann, Wolfgang; Braess, Jan

doi:10.1007/s00063-007-1036-1

Cited by 4 publications

(6 citation statements)

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“…The cytogenetics and gene expression status of these 50 patients at the time of initial diagnosis were obtained by consulting the hospital's electronic medical record system. For prognostic grouping based on cytogenetics, ALL patients use the grouping criteria for prognostic factors of adult acute lymphoblastic leukemia (Rowe, 2010), and AML patients use the Cancer and Leukemia Group B (CALGB) grouping standard (Heilmeier et al, 2007). When comparing the differences between the two groups of AML patients, we adopted the 2017 European Leukemia Network (ELN) risk stratification recommendations (Dohner et al, 2017).…”

Section: Patients and Specimen Collectionmentioning

confidence: 99%

Serum Metabolomics Coupling With Clinical Laboratory Indicators Reveal Taxonomic Features of Leukemia

et al. 2022

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Metabolic abnormality has been considered to be the seventh characteristic in cancer cells. The potential prospect of using serum biomarkers metabolites to differentiate ALL from AML remains unclear. The purpose of our study is to probe whether the differences in metabolomics are related to clinical laboratory-related indicators. We used LC-MS-based metabolomics analysis to study 50 peripheral blood samples of leukemia patients from a single center. Then Chi-square test and T test were used to analyze the clinical characteristics, laboratory indicators and cytokines of 50 patients with leukemia. Correlation analysis was used to explore the relationship between them and the differential metabolites of different types of leukemia. Our study shows that it is feasible to better identify serum metabolic differences in different types and states of leukemia by metabolomic analysis on existing clinical diagnostic techniques. The metabolism of choline and betaine may also be significantly related to the patient’s blood lipid profile. The main enrichment pathways for distinguishing differential metabolites in different types of leukemia are amino acid metabolism and fatty acid metabolism. All these findings suggested that differential metabolites and lipid profiles might identify different types of leukemia based on existing clinical diagnostic techniques, and their rich metabolic pathways help us to better understand the physiological characteristics of leukemia.

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Section: Patients and Specimen Collectionmentioning

confidence: 99%

Serum Metabolomics Coupling With Clinical Laboratory Indicators Reveal Taxonomic Features of Leukemia

et al. 2022

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“…Mit einer Sensitivität von 10 -3 bis 10 -6 stellt die nested PCR die derzeit sensitivste Methode für den Nachweis minimaler Resterkrankung (MRD) dar [6]. Im Gegensatz zur herkömmlichen PCR gewinnt man mit dieser Technik nicht nur die Information, ob eine spezielle molekulargenetische Veränderung vorhanden ist oder nicht, sondern kann sie auch quantifizieren. Zusätzlich zu den spezifischen Primern werden bei dieser Methode fluoreszenzmarkierte Sonden in den Reaktionsansatz gegeben, die während der laufenden PCR mit den sich stetig vermehrenden Amplifikationsprodukten hybridisieren und dann Fluoreszenzsignale abgeben.…”

Section: Polymerase-kettenreaktion (Pcr)unclassified

“…Der Zeitpunkt (PCR-Zyklus), zu dem erstmals eine Fluoreszenz detektierbar wird, korreliert mit der Zahl der nachzuweisenden Moleküle im Ausgangsmaterial. Die Sensitivität der Real-time-PCR liegt bei 10 -2 bis 10 -5 [6]. …”

Section: Polymerase-kettenreaktion (Pcr)unclassified

“…Der Nachweis von unspezifischer Esterase im blastären Zytoplasma deutet auf die Zugehörig-keit zur monozytären Reihe hin. Dies erlaubt die Einordnung einer AML in Richtung der FAB-Gruppen M4 und M5[6].Zytomorphologie und Zytochemie gemeinsam erlauben in der großen Mehrzahl der Fälle die Diagnose einer Leukämie und deren Einordnung (akut vs. chronisch; myeloisch vs. lymphatisch). Diese Methodik ist deshalb der erste Schritt in der speziellen Diagnostik von Leukämien aus Knochenmark und Blut.…”

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“…6 Darstellung von Genexpressionsprofilen verschiedener AML-Subtypen durch die DNA-Mikroarraytechnik (nk: normaler Karyotyp). Jedes kleine Rechteck markiert die Expression eines Gens, rote Visualisierung zeigt dabei eine hohe und grüne eine niedrige Expression an.…”

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Moderne Leukämiediagnostik beim Erwachsenen

Heilmeier

Spiekermann

Bohlander

et al. 2009

Dtsch med Wochenschr

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Identification of numerous criteria important in the pathogenesis, biology, prognosis and treatment of the different types of leukemia necessitates a broad spectrum of diagnostic methods for the initial diagnosis and in the further course of the disease. In addition to cytomorphology with cytochemistry, which is been path-breaking for the application of further diagnostic methods, cytogenetics has become an obligatory diagnostic tool. Immunophenotyping and, even more relevant, molecular genetics plays an important role. Other diagnostic techniques are widely developed. The diagnostic procedures are described, with a focus on their mode of operation as well as their clinical significance. Because of their high clinical relevance and growing complexity, the diagnosis of leukemias should be performed in specialized laboratories.

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Current Awareness in Hematological Oncology

2007

Hematological Oncology

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of hematological oncology. Each bibliography is divided into 14 sections: 1 Reviews; 2 General; Leukemias: 3 Lymphoblastic; 4 Myeloid & Myelodysplastic Syndromes; 5 Chronic; 6 Others; Lymphomas: 7 Hodgkin's; 8 Non‐Hodgkin's; 9 Plasmacytomas/Multiple Myelomas; 10 Others; 11 Bone Marrow Transplantation; 12 Cytokines; 13 Diagnosis; 14 Cytogenetics. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted.

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Diagnostik, Klassifikation und Prognosefaktoren der akuten myeloischen Leukämie

Abstract: A wide range of prognostic criteria of AML is discussed on the basis of currently available clinical data. The most important criteria are the karyotype of the leukemic clone and the patient's age.

Cited by 4 publications

References 52 publications

Serum Metabolomics Coupling With Clinical Laboratory Indicators Reveal Taxonomic Features of Leukemia

Serum Metabolomics Coupling With Clinical Laboratory Indicators Reveal Taxonomic Features of Leukemia

Moderne Leukämiediagnostik beim Erwachsenen

Current Awareness in Hematological Oncology

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