Diagnostics and Therapy Assessment Using Label-Free Raman Imaging

El-Mashtoly, Samir F.; Gerwert, Klaus

doi:10.1021/acs.analchem.1c04483

Cited by 22 publications

(22 citation statements)

References 180 publications

(397 reference statements)

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“…43 Interestingly, machine-learning approaches are also becoming increasingly important to help analyze Raman spectral data in such methods. 44 Functional imaging is also a current important field of research for label-free imaging. In 2010, a pioneer work has been performed showing that ion fluxes can be monitored with digital holography, the signal being carried by the change in refractive index due to osmotic equilibrium.…”

Section: Complex Sample At the Core Of The Image And Ensuring Quantit...mentioning

confidence: 99%

“…Although penalized by weak signals, low readout speed, and thorough signal processing, this fascinating field of research, from spontaneous Raman scattering to stimulated Raman spectroscopy, is attracting highly deserved attention for bioimaging with application in biomedicine, for example, for cancer and drug characterization . Interestingly, machine-learning approaches are also becoming increasingly important to help analyze Raman spectral data in such methods …”

Section: Some Next Challenges: Placing the Complex Sample At The Core...mentioning

confidence: 99%

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On Some Current Challenges in High-Resolution Optical Bioimaging

Bon

Cognet

2022

ACS Photonics

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In this Perspective we propose our current point of view and a suggestive roadmap on the field of high-resolution optical microscopy dedicated to bioimaging. Motivated by biological applications, researchers have indeed devised an impressive amount of strategies to address the diverse constraints of imaging and studying biological matter down to the molecular scale, making this interdisciplinary research field a vibrant forum for creativity. Throughout the discussion, we highlight several striking recent successes in this quest. We also identify some next challenges still ahead to apprehend biological questions in increasingly complex living organisms for integrative studies in a minimally invasive manner.

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Section: Complex Sample At the Core Of The Image And Ensuring Quantit...mentioning

confidence: 99%

Section: Some Next Challenges: Placing the Complex Sample At The Core...mentioning

confidence: 99%

On Some Current Challenges in High-Resolution Optical Bioimaging

Bon

Cognet

2022

ACS Photonics

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“…To overcome this hurdle, Resonance Raman (RR), Surface-Enhanced Raman Spectroscopy (SERS), and Coherent Raman spectroscopy (CRS) were developed. Scientists have mostly adopted SERS in nanomedicine due to its signal enhancement in the presence of rough surface nanomaterials such as Au, Ag, and Cu in the sample system [ 134 ].…”

Section: Graphene Nanocomposite Theranostics For Multimodal Imaging G...mentioning

confidence: 99%

Multimodal Imaging and Phototherapy of Cancer and Bacterial Infection by Graphene and Related Nanocomposites

2022

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The advancements in nanotechnology and nanomedicine are projected to solve many glitches in medicine, especially in the fields of cancer and infectious diseases, which are ranked in the top five most dangerous deadly diseases worldwide by the WHO. There is great concern to eradicate these problems with accurate diagnosis and therapies. Among many developed therapeutic models, near infra-red mediated phototherapy is a non-invasive technique used to invade many persistent tumors and bacterial infections with less inflammation compared with traditional therapeutic models such as radiation therapy, chemotherapy, and surgeries. Herein, we firstly summarize the up-to-date research on graphene phototheranostics for a better understanding of this field of research. We discuss the preparation and functionalization of graphene nanomaterials with various biocompatible components, such as metals, metal oxides, polymers, photosensitizers, and drugs, through covalent and noncovalent approaches. The multifunctional nanographene is used to diagnose the disease with confocal laser scanning microscopy, magnetic resonance imaging computed tomography, positron emission tomography, photoacoustic imaging, Raman, and ToF-SMIS to visualize inside the biological system for imaging-guided therapy are discussed. Further, treatment of disease by photothermal and photodynamic therapies against different cancers and bacterial infections are carefully conferred herein along with challenges and future perspectives.

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“…Despite the power of targeted spatial proteomics methods, they are limited by the costly and laborious process of the development and validation of affinity reagents . Furthermore, antibody-based imaging techniques have limited capabilities for targeting post-translational modifications (PTMs). , Label-free approaches have attracted considerable attention in studies focused on the untargeted protein imaging including the discovery of disease biomarkers and understanding of complex biochemical pathways associated with health and disease. − Raman spectroscopy imaging is a well-established noninvasive label-free imaging modality, which has been widely used for imaging of living cells with subcellular spatial resolution. , Although Raman spectra of individual proteins contain characteristic features in the fingerprint region, substantial spectral overlap presents a challenge to the identification of protein signals observed in imaging experiments. ,, …”

Section: Introductionmentioning

confidence: 99%

Nano-DESI Mass Spectrometry Imaging of Proteoforms in Biological Tissues with High Spatial Resolution

Yang

Unsihuay

et al. 2023

Anal. Chem.

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Mass spectrometry imaging (MSI) is a powerful tool for label-free mapping of the spatial distribution of proteins in biological tissues. We have previously demonstrated imaging of individual proteoforms in biological tissues using nanospray desorption electrospray ionization (nano-DESI), an ambient liquid extraction-based MSI technique. Nano-DESI MSI generates multiply charged protein ions, which is advantageous for their identification using top-down proteomics analysis. In this study, we demonstrate proteoform mapping in biological tissues with a spatial resolution down to 7 μm using nano-DESI MSI. A substantial decrease in protein signals observed in high-spatialresolution MSI makes these experiments challenging. We have enhanced the sensitivity of nano-DESI MSI experiments by optimizing the design of the capillary-based probe and the thickness of the tissue section. In addition, we demonstrate that oversampling may be used to further improve spatial resolution at little or no expense to sensitivity. These developments represent a new step in MSI-based spatial proteomics, which complements targeted imaging modalities widely used for studying biological systems.

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Diagnostics and Therapy Assessment Using Label-Free Raman Imaging

Cited by 22 publications

References 180 publications

On Some Current Challenges in High-Resolution Optical Bioimaging

On Some Current Challenges in High-Resolution Optical Bioimaging

Multimodal Imaging and Phototherapy of Cancer and Bacterial Infection by Graphene and Related Nanocomposites

Nano-DESI Mass Spectrometry Imaging of Proteoforms in Biological Tissues with High Spatial Resolution

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