Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives

Abstract

The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induced serious public healthcare issues and significantly weakened the global economy. Although infecting SARS-CoV-2 will not be as fatal...

Dual Signal Amplification by Urease Catalysis and Silver Nanoparticles for Ultrasensitive Colorimetric Detection of Nucleic Acids

“…Improving the assay sensitivity of biosensors is of vital importance to detect targets at ultra-trace levels for the early diagnosis of diseases, biomedicine, and food industries. − To achieve this goal, target and signal amplification is commonly required. The polymerase chain reaction (PCR) can amplify a specific DNA strand by a factor of 10 6 and be widely considered as the gold standard, however, constrained by thermal cycling .…”

Sensitive Lateral Flow Immunoassay Based on Specific Peptide and Superior Oxidase Mimics with a Universal Dual-Mode Significant Signal Amplification

“…36,37 As the epidemic evolves and the virus mutates, the prevention and treatment of SARS-CoV-2 should be further strengthened to improve the accuracy and sensitivity of diagnostic methods. 38,39 Therefore, in this work, selecting SARS-CoV-2 as a proof of concept, we proposed a nonamplified bioscreening approach to identify peptides that can bind to the SARS-CoV-2 S protein with good affinity and specificity. Subsequently, we constructed the antibody-free pLFIA system using the identified peptide as a capture probe and developed a PDA@MnO 2 -based pLFIA with outstanding oxidase-like activity as a colorimetric tag for significant signal enhancement.…”

“…Polydopamine (PDA), possessing chemical and physical properties similar to those of natural melanin, along with strong adhesion and excellent biocompatibility, is considered to be a typical class of artificial melanin materials. − In addition, MnO 2 and MnO 2 -related materials exhibit prominent oxidase-like activities, which can catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation with remarkable on-demand signal enhancement . The catastrophic spread of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused serious public healthcare problems and severely weakened the global economy. , As the epidemic evolves and the virus mutates, the prevention and treatment of SARS-CoV-2 should be further strengthened to improve the accuracy and sensitivity of diagnostic methods. , Therefore, in this work, selecting SARS-CoV-2 as a proof of concept, we proposed a nonamplified bioscreening approach to identify peptides that can bind to the SARS-CoV-2 S protein with good affinity and specificity. Subsequently, we constructed the antibody-free pLFIA system using the identified peptide as a capture probe and developed a PDA@MnO 2 -based pLFIA with outstanding oxidase-like activity as a colorimetric tag for significant signal enhancement.…”

Aptamer-based kinetically controlled DNA reactions coupled with metal–organic framework nanoprobes for sensitive detection of SARS-CoV-2 spike protein

“…Emerging aptamers with good binding affinity and specificity provide opportunities for the establishment of detection methods for SARS-CoV-2 spike protein. 13–20 For example, Metternich et al made use of two aptamers targeting SARS-CoV-2 spike protein to develop a fluorescence detection method based on covalent guanine quantum defect chemistry; 15 Luo et al demonstrated the usability of aptamer-functionalized MXene nanosheets as a novel sensor that allowed detection of SARS-CoV-2 spike protein down to 38.9 fg mL −1 ; 17 Xue et al reported an electrochemical method based on aptamer-binding induced multiple hairpin assembly and successfully applied it to detect as low as 9.79 fg mL −1 SARS-CoV-2 spike protein. 20 Although these methods have shown promising feasibility, they still have some limitations.…”