Diagnóstico microbiológico y vigilancia epidemiológica de la campilobacteriosis en Chile: Situación actual y desafíos futuros

Collado, Luís

doi:10.4067/s0716-10182020000300244

Cited by 7 publications

(15 citation statements)

References 18 publications

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“…In countries such as Chile, even though Campylobacter is a notifiable enteric pathogen under active surveillance by public health agencies, routine stool culture–testing for this pathogen is rarely performed. This is partially explained by the high costs associated with the culture of this bacterium, including the need for special selective media and specific temperature and microaerophilic growth conditions [ 50 ]. As a consequence, there is limited genomic, clinical and epidemiological data available from the region, leaving an important knowledge gap in our understanding of the global population structure, virulence potential, and antimicrobial resistance profiles of clinical Campylobacter strains [ 1 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic analysis of the diversity, antimicrobial resistance and virulence potential of clinical Campylobacter jejuni and Campylobacter coli strains from Chile

et al. 2021

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Campylobacter jejuni and Campylobacter coli are the leading cause of human gastroenteritis in the industrialized world and an emerging threat in developing countries. The incidence of campylobacteriosis in South America is greatly underestimated, mostly due to the lack of adequate diagnostic methods. Accordingly, there is limited genomic and epidemiological data from this region. In the present study, we performed a genome-wide analysis of the genetic diversity, virulence, and antimicrobial resistance of the largest collection of clinical C. jejuni and C. coli strains from Chile available to date (n = 81), collected in 2017–2019 in Santiago, Chile. This culture collection accounts for more than one third of the available genome sequences from South American clinical strains. cgMLST analysis identified high genetic diversity as well as 13 novel STs and alleles in both C. jejuni and C. coli. Pangenome and virulome analyses showed a differential distribution of virulence factors, including both plasmid and chromosomally encoded T6SSs and T4SSs. Resistome analysis predicted widespread resistance to fluoroquinolones, but low rates of erythromycin resistance. This study provides valuable genomic and epidemiological data and highlights the need for further genomic epidemiology studies in Chile and other South American countries to better understand molecular epidemiology and antimicrobial resistance of this emerging intestinal pathogen.

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Section: Discussionmentioning

confidence: 99%

Genomic analysis of the diversity, antimicrobial resistance and virulence potential of clinical Campylobacter jejuni and Campylobacter coli strains from Chile

et al. 2021

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“…Stool culture is the diagnostic standard for Campylobacter spp. ; however, the high costs and complexity of this method have prevented its routine implementation in many resource-limited countries, although the pathogen seems to be of emerging epidemiological relevance in these regions [ 5 , 6 ]. The epidemiology and spectrum of campylobacteriosis in South America are uncertain [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Epidemiological studies in Chile demonstrated prevalence rates from 0.4%, using microscopy, to 18%, using culture [ 8 ]. A clinical study showed an increase in the detection of campylobacteriosis from 0.4% to 6.1% after the implementation of culture methods within routine stool workflow [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Campylobacter spp. Prevalence in Santiago, Chile: A Study Based on Molecular Detection in Clinical Stool Samples from 2014 to 2019

et al. 2023

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Campylobacter spp. is an emerging cause of infectious diarrhea worldwide. In South American countries such as Chile, its prevalence is underestimated due to inadequate detection methods. Gastrointestinal multiplex PCR panels (GMP) permit rapid and sensitive detection of bacterial pathogens and provide important epidemiological information. This study aimed to analyze Campylobacter epidemiology using the results of molecular methods and to compare molecular detection results to those of culture methods. We performed a retrospective, descriptive analysis of Campylobacter spp. detected in clinical stool samples between 2014–2019 by GMP and culture. Within 16,582 specimens examined by GMP, Campylobacter was the most prevalent enteropathogenic bacteria (8.5%), followed by Salmonella spp. (3.9%), Shigella spp./enteroinvasive Escherichia coli (EIEC) (1.9%), and Yersinia enterocolitica (0.8%). The highest Campylobacter prevalence occurred in 2014/2015. Campylobacteriosis affected more males (57.2%) and adults from 19–65 years (47.9%) and showed a bimodal seasonality with summer and winter peaks. In 11,251 routine stool cultures, Campylobacter spp. was detected in 4.6%, mostly C. jejuni (89.6%). Among 4533 samples tested by GMP and culture in parallel, GMP showed a superior sensitivity (99.1% versus 50%, respectively). The study suggests that Campylobacter spp. is the most frequent bacterial enteropathogen in Chile.

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“…Resistance to ciprofloxacin (30–60%), tetracycline (2–24%) and erythromycin (2–6%) has been reported in clinical isolates of C. jejuni in Chile (Collado 2020). However, the origin of this resistance is unknown, although it has been suggested that it may originate in part from the Chilean poultry industry, due to the high rates of resistance previously found in Campylobacter spp.…”

Section: Introductionmentioning

confidence: 99%

Campylobacter jejuni and other emerging Campylobacteraceae in retail beef liver – an underestimated potential source?

Lopez-Cantillo

Opazo-Capurro

López-Joven

et al. 2022

Letters in Applied Microbiology

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Bovine by‐products, such as liver, could be an underestimated source of Campylobacter jejuni. Therefore, our aims were to evaluate the occurrence of C. jejuni and other Campylobacteraceae in retail beef liver and characterize their antibiotic resistance (ciprofloxacin, tetracycline, erythromycin and gentamicin) and potential genetic relationship by flagellin gene restriction fragment length polymorphism (flaA‐RFLP) and multilocus sequence typing with clinical strains. Seventy‐six out of 206 samples (36·9%) were positive for Campylobacter and related organisms. Arcobacter butzleri was the most frequently isolated species (21·8%), followed by C. jejuni (9·7%), C. fetus (7·8%) and C. coli (1%). The C. jejuni strains showed resistance to tetracycline (17·2%) or ciprofloxacin (6·9%), with only one strain resistant to both antibiotics. Meanwhile, 8·3% of ciprofloxacin resistance was observed in C. fetus. The other species showed no resistance. Most of the clonal complexes (CC) in which the C. jejuni genotypes were grouped (CC‐21, 42, 48 and 52), coincided with genotypes of clinical strains previously reported in Chile. As such, this study provides evidence that beef liver could be an underestimated route for resistant C. jejuni to humans. Further studies should assess whether this food could play a role in the transmission of other emerging Campylobacteraceae such as those reported here.

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Diagnóstico microbiológico y vigilancia epidemiológica de la campilobacteriosis en Chile: Situación actual y desafíos futuros

Abstract: El autor declara no tener conflictos de interés en este tema. Este trabajo fue financiado por Fondo Nacional de Ciencia y Tecnología de Chile (FONDECYT 1200125). Además, el autor agradece el soporte del Núcleo Milenio Biología de la Microbiota Intestinal (NU-GUTmicro).

Cited by 7 publications

References 18 publications

Genomic analysis of the diversity, antimicrobial resistance and virulence potential of clinical Campylobacter jejuni and Campylobacter coli strains from Chile

Genomic analysis of the diversity, antimicrobial resistance and virulence potential of clinical Campylobacter jejuni and Campylobacter coli strains from Chile

Campylobacter spp. Prevalence in Santiago, Chile: A Study Based on Molecular Detection in Clinical Stool Samples from 2014 to 2019

Campylobacter jejuni and other emerging Campylobacteraceae in retail beef liver – an underestimated potential source?

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