Diagnostical Appreciation of Physiological Reaction of Intravascular Thrombocytes’ Activity of Two-Years-Old Mice to Regular Physical Loads

Zavalishina, Svetlana Yuryevna; Vatnikov, Yury Anatolevich; Makurina, O.N.; Kulikov, Evgeny Vladimirovich; Sotnikova, E.D.; Паршина, Валентина; Rystsova, Ekaterina Olegovna; Kochneva, Margarita Vasil'evna; Sturov, Nikolaj Vladimirovich

doi:10.13005/bpj/1090

Cited by 6 publications

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“…In the dissociation of a homogeneous population into S- and R-forms that differ from random mutations by a high frequency of reversion, the variability of genetic, morphological, physiological, and antigenic properties is observed, and the decrease in metabolic processes and the transition of the population to the “uncultivated state” cause the duration and retrospective of bacteriological studies [ 1 ]. S-variants contain all the components of lipopolysaccharides; R-variants express an incomplete polysaccharide; lowering the level of acylation of lipids leads to a decrease in hydrophobicity and an increase in fluidity and the permeability of the outer membrane, which activates the collection, transport, and exchange of lipophilic compounds of cells of susceptible species [ 12 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

Features of formation of Yersinia enterocolitica biofilms

et al. 2019

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Aim: The work aimed to study the morphology of colonies and their comparison by features of the formation of Yersinia enterocolitica biofilms. Materials and Methods: Bacteria were cultured on a Yersinia Selective Agar medium ("CIN-agar") at 28°C for 24 h. The microorganisms were grown in meat-peptone broth with 1.0% glucose to measure the absolute values of the optical density of the culture. The optical density of the liquid was determined in a microplate photometric analyzer Immunochem-2100 (HTI, USA) at a wavelength of 490 nm. For the study of biofilms, the specimens were fixed for 3-5 h in pairs of 25.0% solution of glutaraldehyde (according to DV), and pairs of a 1.0% aqueous solution of osmic acid (OSO4) were used for contrasting for 2-3 min. The specimens were examined with stereoscopic microscopy "BIOMED MS-1 Stereo" (Russia) and scanning electron microscope "TM 3030 plus" (Holland). Results: With stereoscopic microscopy of the colonies of Y. enterocolitica, the S-forms had an elevated intensely colored center, radial striation along the periphery, smooth edges, d ≤ 1.0 mm. R-form colonies had a dark color and a dry surface, were tuberous and had a dense center with a peripheral ridge, rugged edges, d ≥ 1.5 mm. The optical density of the Y. enterocolitica S-form showed that this type of microorganism belongs to the moderate producers of biofilms since the optical density of the sample (density of the sample - Ds) exceeded the optical density of control (density of the control - Dc) by 3 times. In Y. enterocolitica R-form (D ≤ 0.197) weakly produced biofilms, the optical density of the sample exceeded the optical density of the control by <2 times. Conclusion: The ability to form biofilms, the variability of phenotypic features, and the multiplicity of virulence factors of bacteria significantly reduce the effectiveness of diagnostic studies. The development of accelerated methods of detection and differentiation of the virulent properties of pathogenic bacteria will allow scientifically to substantiate and develop a set of measures aimed at preventing animal diseases and obtaining safe livestock products to prevent human diseases. Thus, we need to pay attention to which forms of colonies do Y. enterocolitica form on solid nutrient media: S- or R-forms. Through this study, we know that bacteria-forming S-shaped colonies are more capable of forming biofilms than R-forms. It means that they are more pathogenic and can cause persistent infections due to adhesion and biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Features of formation of Yersinia enterocolitica biofilms

et al. 2019

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Section: Introductionmentioning

confidence: 92%

“…The immunological concept of the pathogenesis of chronic heart failure is based on the elevation of certain laboratory parameters (erythrocyte sedimentation rate, neutrophil numbers, C-reactive protein, and pro-inflammatory cytokines) detected in patients with cardiac disease, which is consistent with chronic aseptic inflammation [4,[11][12][13]. These abnormalities have been confirmed in laboratory experimental models [14]. In addition to the toxic effects of the products of aseptic inflammation, stasis and hypoxia of the stomach and intestines lead to the development of malabsorption syndrome, impaired tissue metabolism, and reduced liver detoxification.…”

Section: Introductionmentioning

confidence: 99%

Immune-inflammatory concept of the pathogenesis of chronic heart failure in dogs with dilated cardiomyopathy

et al. 2019

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Background: Dilated cardiomyopathy is common in dogs. This form of cardiomyopathy is the main cause of death due to heart disease in dogs. Death can occur suddenly in clinically normal animals as a result of the progression of congestive heart failure (CHF). The pathogenesis of heart failure syndrome in dogs with dilated cardiomyopathy involves activation of the neurohumoral system and immune-mediated inflammation, which leads to further progression of the condition. Heart failure syndrome in dogs with dilated cardiomyopathy is caused by the progressive loss of cardiomyocytes, apoptosis, remodeling of the left ventricle, systolic and diastolic dysfunction, arrhythmias, reduced cerebral blood flow, the involvement of other key internal organs, and intestinal dysbiosis. Aim: This study aimed to determine the immunological and inflammatory mechanisms surrounding the development of heart failure syndrome in dogs with dilated cardiomyopathy. Materials and Methods: The subjects of this study were dogs with a dilated form of cardiomyopathy (n=159), complicated by various functional classes of heart failure syndrome. Evaluation of myocardial remodeling, systolic function, and systemic hemodynamics was performed using EMP-860 Vet and PU-2200V ultrasound scanners according to the standard technique. Electrocardiography was performed with all dogs in right lateral recumbency using the EK1T-04 Midas electrocardiograph (50 mm/s speed and 1 mV gain = 1 cm). Results: In some affected animals, especially in cases of compensated dilated cardiomyopathy, leukocytosis was noted. In patients with dilated cardiomyopathy complicated by heart failure syndrome of various functional classes, the number of neutrophils was significantly increased, and the number of lymphocytes was decreased by 1.9-2.1 times when compared with those in clinically normal animals. In dogs with dilated cardiomyopathy, neutrophilic leukocytosis develops with a simple regenerative shift to the left. The results of immunological studies indicate that dogs with dilated cardiomyopathy develop T lymphocytopenia as compared with clinically normal animals. Conclusion: The central component of heart failure syndrome in dogs with dilated cardiomyopathy is the activation of the neurohumoral system and immune-mediated inflammation. The development of CHF in dogs with dilated cardiomyopathy is caused by the progressive loss of cardiomyocytes, apoptosis, remodeling of the left ventricle, systolic and diastolic dysfunction, arrhythmias, reduced cerebral blood flow, involvement of other key internal organs, and intestinal dysbiosis.

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“…Hereditary and acquired pathologies in animals of both non-infectious and contagious etiology are very diverse and complex, characterized by various forms of manifestations, which are often difficult to diagnose and require special approaches in treatment and prevention [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

“…It should be noted that most dogs with endocardiosis have an asymptomatic course for many years or even throughout their lives [4,9,10]. However, with progressive mitral regurgitation, severe complications can arise in the form of development of left-sided and then rightsided congestive chronic heart failure (CHF), usually secondary to the high pulmonary arterial hypertension syndrome [5]. However, in dogs with endocardiosis, the tricuspid valve is often affected leading to the development of tricuspid regurgitation [7,11].…”

Section: Introductionmentioning

confidence: 99%

Left ventricular myocardial remodeling in dogs with mitral valve endocardiosis

et al. 2020

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Background and Aim: Left ventricular myocardial remodeling could play an important role in the progression of chronic heart failure (CHF) syndrome in dogs with mitral valve endocardiosis. The aim of this study was to evaluate the left ventricular myocardial remodeling in dogs with mitral valve endocardiosis and to study the dependence of the incidence of this pathological phenomenon on the functional class (FC) of progression of the CHF syndrome. Materials and Methods: A total of 108 afflicted dogs and 36 clinically healthy dogs were examined using transthoracic echocardiography. The following structural and geometric parameters of the left ventricular remodeling were evaluated: Myocardial mass and its index, sphericity index at the end of systole and diastole, end-systolic and end-diastolic relative wall thickness, and integral remodeling index. Results: In all clinically healthy dogs, a normal type of the left ventricular chamber geometry was revealed, whereas, in dogs with mitral valve endocardiosis, the normal geometry of the left ventricle occurred in 56.4%, eccentric hypertrophy in 24.1%, concentric remodeling in 10.2%, and concentric hypertrophy in 9.3% of the cases. In patients with endocardiosis, there was no dilatation type of cardiac remodeling observed. Conclusion: When compared to the clinically healthy animals, the dogs with mitral valve endocardiosis presented with indicators of structural and geometric remodeling, such as increased myocardial mass, myocardial mass index, and sphericity index at the end of systole and diastole, as well as relatively reduced integral systolic index of remodeling and systolic relative thickness of the walls of the heart. The parameters of the left ventricular myocardial remodeling correlated significantly with the FC of CHF syndrome.

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Diagnostical Appreciation of Physiological Reaction of Intravascular Thrombocytes’ Activity of Two-Years-Old Mice to Regular Physical Loads

Cited by 6 publications

References 0 publications

Features of formation of Yersinia enterocolitica biofilms

Features of formation of Yersinia enterocolitica biofilms

Immune-inflammatory concept of the pathogenesis of chronic heart failure in dogs with dilated cardiomyopathy

Left ventricular myocardial remodeling in dogs with mitral valve endocardiosis

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