Diagnostic Yield of Multiplex PCR Method in Cerebrospinal Fluid for the Diagnosis of Purulent Meningitis in Children

Zhao, Jing-Li; Chen, Hua; Xie, Yi; Pan, Yan-Xiang; Hu, Bo-Fei; Wang, Weijian; He, X. T.

doi:10.1055/s-0040-1719163

Cited by 3 publications

(2 citation statements)

References 22 publications

(20 reference statements)

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“…Although there is no data available describing the accuracy of PCR testing in all microorganisms causing meningitis, common microorganisms had been found to have significant variability with 61–100% sensitivity for Streptococcus pneumoniae , 72−92% for Haemophilus influenzae , and 88–94% for Neisseria meningitides ( 9 ). The reported sensitivity of S. agalactiae using ME PCR panel was found to be near 100% ( 32 , 33 ). This result was not in concordance with the previously reported sensitivity of the targeted specific gene real-time PCR used to detect a specific GBS serotype as some reports found it less sensitive with a 40% chance of missing the detection.…”

Section: Discussionmentioning

confidence: 99%

Case Report: Prolonged CSF PCR Positivity in a Neonate With GBS Meningitis

et al. 2021

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Bacterial meningitis is one of the critical diseases that needs to be diagnosed and treated promptly. Recent diagnostics of high sensitivity and specificity rates, such as PCR, helped with such presentation, especially in cases with prior antibiotics that led to culture negativity. However, the time window of PCR positivity is not well-studied, with scattered reports of different periods of positivity. Here, we report a case of neonatal GBS meningitis with positive PCR for more than 80 days from starting antibiotics.

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Section: Discussionmentioning

confidence: 99%

Case Report: Prolonged CSF PCR Positivity in a Neonate With GBS Meningitis

et al. 2021

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“…The primers for RT-PCR amplification of the eight viruses are shown in Table 1 . The workflow of our multiplex RT-PCR system and conditions used in the PCR reaction cycle is described in our previous study [ 6 ]. PCR products were then analyzed using advanced fragment analysis (AFA).…”

Section: Methodsmentioning

confidence: 99%

Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019

et al. 2022

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Objective: To investigate the common pathogens of viral encephalitis (VE) in children, and to provide guidance for the empirical diagnosis and treatment of patients with VE. Methods: A total of 227 cerebrospinal fluid (CSF) samples were collected from pediatric patients with VE in Zhejiang province from January 2018 to December 2019. The samples were tested using multiplex and singleplex Reverse Transcription-Polymerase Chain Reaction (RT-PCR) with primers specific to enterovirus (EV), varicella-zoster virus (VZV), mumps virus (MuV), cytomegalovirus (CMV), herpes simplex virus type 1 (HSV-1)/type 2 (HSV-2), Epstein–Barr virus (EBV), and human herpesvirus 6 (HHV-6). The data of the two analyses were compared and then verified using Sanger sequencing. Results: Of the 227 CSF samples, 90 were shown to be positive for multiplex RT-PCR with a positivity rate of 39.65% and a 95% confidence interval (33.2%, 46.1%). EV was the most common cause of VE, followed by EBV, HHV-6, MuV, CMV, VZV, and HSV-1. Most included cases occurred in summer, accounting for 49.78% of all cases. For EV, EBV, and HSV-2, multiplex RT-PCR showed a positivity rate of 34.36%, which was not statistically different from that of 30.4% shown by singleplex RT-PCR. The sequences of EV, EBV, VZV, MuV, CMV, HSV-1, HHV-6, and HSV-2 were confirmed by sequencing the PCR products obtained from multiplex and singleplex PCR. Conclusions: In children, VE is more prevalent in the summer than in other seasons in Zhejiang province, and EV may be the most common causative pathogen.

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Evaluation of the accuracy of multiplex polymerase chain reaction in differentiation between bacterial and viral meningitis

et al. 2022

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Background Meningitis is one of the most dangerous infection affecting children. The need for rapid and accurate diagnosis is mandatory for improving the outcome. Aim of the work To evaluate the role of multiplex polymerase chain reaction (PCR) in diagnosis of meningitis either bacterial or viral and to detect its accuracy. Patients and methods A cross-sectional study was carried out in University Children Hospital, Faculty of Medicine, between November 2019 and September 2020. The study was approved by the Ethics Review Board of Faculty of Medicine, Assiut University, and informed written consent was obtained. The committee’s reference number is 17200161. Clinicaltrails.gov ID: NCT03387969. Forty-eight children aged 2 to 18 years with meningitis were included. Detailed history and examination, blood glucose level at time of admission prior to lumbar puncture, and multiplex PCR in cerebrospinal fluid (CSF) were evaluated. Results The mean age of children was 3.27 ± 1.27 years. Thirty-five (72.9%) cases were bacterial meningitis while 13 (27.1%) cases were viral meningitis. Multiplex PCR had 94% sensitivity and 100% specificity for diagnosis of bacterial meningitis. Conclusion Multiplex PCR may help in diagnosis and differentiation of bacterial and viral meningitis with accurate and rapid results. What is Known:• Rapid and accurate diagnosis is mandatory for successful treatment of meningitis.• Differentiation between bacterial and viral meningitis is important to avoid unnecessary treatment.. What is New:• Multiplex PCR is an important marker for rapid detection of meningitis.• Multiplex PCR may be used to differentiate between bacterial and non-bacterial cause of meningitis.

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Diagnostic Yield of Multiplex PCR Method in Cerebrospinal Fluid for the Diagnosis of Purulent Meningitis in Children

Cited by 3 publications

References 22 publications

Case Report: Prolonged CSF PCR Positivity in a Neonate With GBS Meningitis

Case Report: Prolonged CSF PCR Positivity in a Neonate With GBS Meningitis

Etiological Analysis of Viral Encephalitis in Children in Zhejiang Province from 2018 to 2019

Evaluation of the accuracy of multiplex polymerase chain reaction in differentiation between bacterial and viral meningitis

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