Diagnostic value of serum sphingolipids in patients with colorectal cancer

Abstract

Background: Abnormal lipid metabolism affects the regulation of tumor progression, though use of serum lipids and sphingolipids for disease progression identification is uncertain. Methods: Serum samples from 51 healthy...

The arachidonic acid metabolome reveals elevation of prostaglandin E2 biosynthesis in colorectal cancer

“…CRC progression is often accompanied by lipid metabolism reprogramming, such as the metabolism of fatty acids, phospholipids, and sphingolipids. 2,12 The AA metabolic pathway involves a large group of oxidized lipids that participate in inflammatory and neuropsychiatric processes. 19 In addition to being part of lipogenesis and decomposition processes, some oxidized lipids can also act as second messengers in the signaling pathway.…”

“…The metabolite extraction method was performed as previously reported. 12,13 Briefly, for serum extraction, 100 μL of serum was mixed with a cocktail of IS in 550 μL of ice-cold methanol containing 0.01 mol L −1 BHT and vortexed for 2 minutes. If necessary, ultrasonic treatment can help full disaggregation.…”

UHPLC-HRMS-Based Serum Untargeted Lipidomics: PCs and SMs are the Main disturbed lipid markers to Distinguish Colorectal Advanced Adenoma from Cancer

“…Tissue lipidomics research revealed that lysophatidylcholines (LPCs) and phosphatidylcholines (PCs) are the most closely associated indicators for the onset and progression of CRC(28, 29). In addition, serum sphingolipid (SPL) pro les present different characteristics at different stages of CRC (30). Triacylglycerols (TAGs), plasmalogens, and fatty acids (FAs) may be major disordered metabolites in the plasma of CRC patients (23,31).…”