Diagnostic value of plasma and blood cells metagenomic next-generation sequencing in patients with sepsis

Comparison of plasma and blood cell samples in metagenomic next-generation sequencing for identification of the causative pathogens of fever

“…However, in terms of diagnosing bloodstream infections, plasma mNGS demonstrated higher sensitivity than blood cell mNGS (69.82 % vs 53.25 %). Therefore, a combined approach of whole-blood mNGS emerges as a more advanced diagnostic method for bloodstream infections (BSI) [ 16 ]. Building upon these insights, our investigation contributes novel and previously unreported findings regarding the utility of plasma and blood-cell mNGS in pediatric patients with compromised immune systems.…”

“…Recent research conducted by Wu and colleagues in cohorts of patients with sepsis has embarked on a similar study, demonstrating that whole-blood metagenomic next-generation sequencing (mNGS), combining both blood cells and plasma, exhibits a higher sensitivity compared to traditional blood cultures or mNGS using plasma alone. This approach shows great potential in the diagnosis of bloodstream infections (BSI) [ 16 ]; however, to date, such studies remain exceedingly limited, and the value of utilizing different blood components for mNGS testing warrants further exploration. Our study was conducted among pediatric patients with febrile illnesses in hematology/oncology, who differ from previous study cohorts by being immunocompromised with a higher incidence of opportunistic infections.…”