Diagnostic value of PB/KOH/PAS method for identification of primary cancer from metastases

Fakan, F; Adamcová, Jana

doi:10.1016/s0065-1281(81)80073-6

Cited by 5 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In non‐colloid adenocarcinomas of the stomach, sialomucins were non‐O‐acylated, in agreement with some studies, 5 , 22 but not with others 3 . In the mammary gland, non‐colloid adenocarcinomas also contained non‐O‐acylated sialomucins, as previously shown 3 , 22 …”

Section: Discussionsupporting

confidence: 90%

“…5,32 In noncolloid adenocarcinomas of the stomach, sialomucins were non-O-acylated, in agreement with some studies, 5,22 but not with others. 3 In the mammary gland, non-colloid adenocarcinomas also contained non-Oacylated sialomucins, as previously shown. 3,22 As far as we are aware, this is the ®rst histochemical study designed to analyse mucin composition in mucinous adenocarcinomas of the colloid type.…”

Section: Discussionsupporting

confidence: 59%

“…3 In the mammary gland, non-colloid adenocarcinomas also contained non-Oacylated sialomucins, as previously shown. 3,22 As far as we are aware, this is the ®rst histochemical study designed to analyse mucin composition in mucinous adenocarcinomas of the colloid type. The analysis of O-acylation of sialic acid was initially made by means of the PB-KOH-PAS and PAT-KOH-PAS methods.…”

Section: Discussionsupporting

confidence: 59%

“…Mucins are high molecular weight glycoproteins normally secreted by a variety of epithelial cells but aberrantly produced by their neoplastic counterparts 1 . Sialic acid is one of the major components of the carbohydrate chain, and shows distinct O‐acylation patterns in the mucins secreted by adenocarcinomas 2–5 . Sialic acid residues have been implicated in tumour progression and metastasis 6–8 .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mucinous (colloid) adenocarcinomas secrete distinct O‐acylated forms of sialomucins: a histochemical study of gastric, colorectal and breast adenocarcinomas

et al. 2001

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 59%

Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mucinous (colloid) adenocarcinomas secrete distinct O‐acylated forms of sialomucins: a histochemical study of gastric, colorectal and breast adenocarcinomas

et al. 2001

View full text Add to dashboard Cite

show abstract

Histochemical identification of side chain substitutedO-acylated sialic acids: The PAT-KOH-Bh-PAS and the PAPT-KOH-Bh-PAS procedures

et al. 1984

View full text Add to dashboard Cite

Two new methods, based on the original periodic acid-Thionin Schiff-saponification-periodic acid-Basic Fuchsin Schiff (PAT-KOH-PAS) technique of Culling et al. (1976), have been devised for the histochemical identification of side-chain O-acylated sialic acids. In the first of these, the periodic acid-Thionin Schiff-saponification-borohydride reduction-periodic acid-Basic Fuchsin Schiff (PAT-KOH-Bh-PAS) procedure, the specificity of the original PAT-KOH-PAS technique was improved by: (a) extending, when necessary, the initial period of periodate oxidation, (b) increasing the period of exposure to Thionin Schiff reagent from 30 min to 4 h, (c) using a Thionin Schiff reagent prepared by a different method, (d) interposing a borohydride reduction step between the saponification and PAS steps and, (e) extending the period of oxidation in the final PAS step from 10 to 30 min. In the second procedure, the periodic acid-phenylhydrazine-Thionin Schiff-borohydride reduction-periodic acid-Basic Fuchsin Schiff (PAPT-KOH-Bh-PAS), based on the periodic acid-phenylhydrazine-Schiff (PAPS) technique of Spicer (1961), blue Thionin Schiff staining was confined to sialic acid residues with oxidizable side chain vicinal diols by interposing a treatment with 0.5% (w/v) aqueous phenylhydrazine hydrochloride for 2 h at room temperature between the initial periodic acid oxidation and the Thionin Schiff steps of the PAT-KOH-Bh-PAS procedure. These procedures are discussed within the general framework of the methods available for the histochemical identification of side-chain O-acylated sialic acids.

show abstract

Chemical and histochemical studies of normal and diseased human gastrointestinal tract. V. A differential diagnostic method for the histochemical classification of glycoproteins

et al. 1985

View full text Add to dashboard Cite

A differential diagnostic scheme is described for the division of colonic epithelial glycoproteins into eleven histochemically distinct classes. The scheme depends upon the use of seven histochemical techniques which, collectively, permit the differential staining of O-sulphate ester, sialic acid and its side chain O-acyl variants and vicinal diols located on carbohydrate residues other than sialic acids. Elements of the scheme also provide a general approach to the classification of epithelial glycoproteins in anatomic sites other than the colon. Application of the scheme permitted the classification of the epithelial glycoproteins in the mucosa 0.5-5.0 cm from human colonic tumours and provided direct confirmation of previous observations that changes from normal in the relative proportions of either side chain O-acylated sialic acids or sialic acids and O-sulphate esters can occur independently of one another.

show abstract

Diagnostic value of PB/KOH/PAS method for identification of primary cancer from metastases

Cited by 5 publications

References 6 publications

Mucinous (colloid) adenocarcinomas secrete distinct O‐acylated forms of sialomucins: a histochemical study of gastric, colorectal and breast adenocarcinomas

Mucinous (colloid) adenocarcinomas secrete distinct O‐acylated forms of sialomucins: a histochemical study of gastric, colorectal and breast adenocarcinomas

Histochemical identification of side chain substitutedO-acylated sialic acids: The PAT-KOH-Bh-PAS and the PAPT-KOH-Bh-PAS procedures

Chemical and histochemical studies of normal and diseased human gastrointestinal tract. V. A differential diagnostic method for the histochemical classification of glycoproteins

Contact Info

Product

Resources

About