“…Likewise, when one analyzes more recent studies, it is possible to notice a significant decrease in specificity when, in sensitized protocols, the duration of the passive phase is equal to or more than 30 minutes. Protocols with shorter passive phases, or even without a passive phase, followed by sensitization with nitrate or isoproterenol, are linked with specificities that range from 84 to 97% 18,28,38 , whereas, in studies that used a more prolonged passive phase, there was a decrease in specificities (48 to 70%) 9,11,15 . Therefore, limiting the total duration of the examination seems to guarantee good specificity.…”