Diagnostic value of miR-186-5p for carotid artery stenosis and its predictive significance for future cerebral ischemic event

Abstract

Background MicroRNAs (miRNAs) serve as novel promising biomarkers for the diagnosis and prognosis of many human diseases. This study investigated the diagnostic value of miR-186-5p for asymptomatic carotid artery stenosis (CAS), and its predictive value for future cerebral ischemic events (CIEs). Methods Sixty-seven cases with asymptomatic CAS and 60 healthy individuals were recruited. Serum levels of miR-186-5p were tested by using qRT-PCR. Receiving–operator characteristic (ROC) curve was drawn based on sensitivity and specificity analyses. All asymptomatic CAS cases were followed up for 5 years. Kaplan-Meier method was applied for the evaluation of the predictive value of miR-186-5p for the occurrence of CIE. Results The serum level of miR-186-5p was increased significantly in asymptomatic CAS patients. MiR-186-5p was the most significant factor associated with the high degree of carotid stenosis in asymptomatic CAS patients. In the ROC curve analysis, the AUC was 0.919, with the sensitivity of 89.6% and specificity of 81.7% at the cutoff value of 1.221. Kaplan-Meier method results revealed that high miR-186-5p level was associated with the occurrence of CIEs. High miR-186-5p level and high degree of carotid stenosis were independent factors for the occurrence of CIEs. Conclusion MiR-186-5p serves as a potential diagnostic biomarker for patients with asymptomatic CAS, and predicts the occurrence of future CIEs.

Aberrant expression of miR‑483‑5p in patients with asymptomatic carotid artery stenosis and its predictive value for cerebrovascular event occurrence

“…In addition, functional miRNAs associated with disease progression are also deregulated in ACAS. For instance, miR-106b-5p ( 13 ) and miR-186-5p ( 22 ) have been indicated to be abnormal in ACAS and associated with ACAS progression. Yan et al ( 23 ) determined that the levels of serum miR-503-5p in patients with ACAS were significantly reduced compared with those in healthy individuals and that miR-503-5p was able to improve CAS.…”

“…For instance, Chen et al ( 14 ) reported that miR-92a may be a potential risk factor for the future onset of cerebrovascular events (including 20 cases of TIA and 8 cases of stroke; study cohort size, 122). A study by Lv et al ( 22 ) determined that miR-186-5p was able to predict the occurrence of future cerebral ischemic events (including 19 TIAs and 5 strokes; study cohort size, 67). Serum miR-106b-5p predicted the occurrence of cerebral ischemic events ( 13 ).…”

Which is worthy to be adopted, carotid endarterectomy or carotid stenting for treatment of symptomatic carotid artery stenosis?

“…The progression of carotid atherosclerosis is slow, and carotid stenosis may remain asymptomatic in many cases (2). However, symptomatic patients may present with transient or permanent contralateral neurologic deficits such as weakness or numbness.…”

miR-28-5p improved carotid artery stenosis by regulating vascular smooth muscle cell proliferation and migration

“…As a small, highly conserved non-coding RNA molecule, microRNAs (miRNAs) can regulate the physiological and pathological processes of disease by inducing target mRNA degradation or blocking translation. 8 In recent years, many studies have shown that a variety of miRNAs are dysregulated in CAS, such as miR-330-5p, 9 miR-186-5p, 10 and miR-214, 11 suggesting that miRNAs have a greater potential regulatory role in CAS. Among the reported miRNAs, miRNA-28-5p attracted our attention.…”

“…CircCBFB promotes the occurrence of abdominal aortic aneurysms by influencing the apoptosis of VSMCs through miRNA-28-5p. 10 Besides, miR-28-5p affects the upregulation of the ABCA1-signaling pathway by regulating ERK2, thus affecting atherosclerosis. 12 Although miR-28-5p can affect common vascular disease, including abdominal aortic aneurysm and atherosclerosis, its effect on the pathogenesis of CAS and its VSMCs in CAS has not been reported.…”