Diagnostic value of maspin in distinguishing adenocarcinoma from benign biliary epithelium on endoscopic bile duct biopsy

Immunocytochemistry for Claudin‐18 and Maspin in biliary brushing cytology increases the accuracy of diagnosing pancreatobiliary malignancies

“…The results of this study indicate that Maspin expression is upregulated in pancreatobiliary carcinomas, being observed in both the cytoplasm and nuclei of cancer cells. These findings are similar to those observed in an immunohistochemical study of samples from patients with endometrioid, lung, pancreatic and bile duct cancers . Maspin immunoreactivity was also observed in the nuclei of benign epithelial cells.…”

“…These findings are similar to those observed in an immunohistochemical study of samples from patients with endometrioid, lung, pancreatic and bile duct cancers. 14,[27][28][29] Maspin immunoreactivity was also observed in the nuclei of benign epithelial cells. These expression patterns may help distinguish malignant from benign epithelial cells.…”

Role of CD15 expression in dysplastic and neoplastic tissue of the bile duct – a potential novel tool for differential diagnosis of indeterminate biliary stricture

“…On the other hand, CD15 might be a suitable instrument for the differential diagnosis of IBS from intraductal biopsies and brush cytologies. Sensitivity in our study is comparable to immunohistochemical studies demonstrating that combined immunohistochemical staining of three different antibodies can differentiate between malignant and non‐malignant bile duct biopsies in 67–86% . This is higher than sensitivity of fluorescence in‐situ hybridization (FISH), which was reported to be 42.9% in a study from 2009 with 498 biliary brush samples .…”

Practical Immunohistochemistry in Neoplastic Pathology of the Gastrointestinal Tract, Liver, Biliary Tract, and Pancreas

“…Maspin.-Maspin is a tumor suppressor that is overexpressed in more than 90% of pancreatic ductal adenocarcinomas, 1 62, 16 7, 18 3,1 84 59% to 100% of gallbladder adenocarcinomas, 168,185 57% to 91% of adenocarcinomas of extrahepatic bile ducts, 186,187 and more than 70% of intrahepatic cholangiocarcinomas. 167,188 Malignant cells typically show strong and diffuse nuclear and cytoplasmic Figure 11.…”

“…Benign pancreatic ducts are usually negative for maspin expression, but benign epithelial cells lining the gallbladder and extrahepatic bile ducts have been shown to be positive at a high frequency. 186,187,189 The staining can thus be difficult to interpret despite the observations that positive staining in benign biliary epithelial cells is usually focal/patchy and weaker in intensity in comparison with that seen in malignant cells.…”