Diagnostic value of lncRNA NORAD in pulmonary tuberculosis and its regulatory role in <i>Mycobacterium tuberculosis</i> infection of macrophages

Sun, Wenna; He, Xiong; Zhang, Xiushuang; Lin, Wen; Liang, Yan

doi:10.1111/1348-0421.12986

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…validated LUCAT1 as a regulator of systemic monocyte immunity during COVID-19 ( 59 ). Another lncRNA, NORAD , has been established to be associated with MTb infection ( 62 ). The nonspecific DE lncRNAs, on the other hand, are involved in mainly DNA repair, cell growth, and cell division pathways.…”

Section: Discussionmentioning

confidence: 99%

Cell-specific housekeeping role of lncRNAs in COVID-19-infected and recovered patients

Chattopadhyay,

Mehta,

Soni

et al. 2024

NAR Genomics and Bioinformatics

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A plethora of studies have demonstrated the roles of lncRNAs in modulating disease severity and outcomes during infection. However, the spatio-temporal expression of these lncRNAs is poorly understood. In this study, we used single-cell RNA-seq to understand the spatio-temporal expression dynamics of lncRNAs across healthy, SARS-CoV-2-infected, and recovered individuals and their functional role in modulating the disease and recovery. We identified 203 differentially expressed lncRNAs, including cell type-specific ones like MALAT1, NEAT1, ZFAS1, SNHG7, SNHG8, and SNHG25 modulating immune function in classical monocyte, NK T, proliferating NK, plasmablast, naive, and activated B/T cells. Interestingly, we found invariant lncRNAs (no significant change in expression across conditions) regulating essential housekeeping functions (for example, HOTAIR, NRAV, SNHG27, SNHG28, and UCA1) in infected and recovered individuals. Despite similar repeat element abundance, variant lncRNAs displayed higher Alu content, suggesting increased interactions with proximal and distal genes, crucial for immune response modulation. The comparable repeat abundance but distinct expression levels of variant and invariant lncRNAs highlight the significance of investigating the regulatory mechanisms of invariant lncRNAs. Overall, this study offers new insights into the spatio-temporal expression patterns and functional roles of lncRNAs in SARS-CoV-2-infected and recovered individuals while highlighting the importance of invariant lncRNAs in the disease context.

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Section: Discussionmentioning

confidence: 99%

Cell-specific housekeeping role of lncRNAs in COVID-19-infected and recovered patients

Chattopadhyay,

Mehta,

Soni

et al. 2024

NAR Genomics and Bioinformatics

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“…A recent study analyzed serum lncRNA NORAD expression in PTB patients and evaluated its potential clinical significance [ 108 ]. These researchers assessed NORAD serum levels using qRT-PCR in 90 PTB patients and 85 healthy individuals.…”

Section: Lncrnas As Potential Biomarkers Of Tbmentioning

confidence: 99%

Advances of Long Non-Coding RNAs as Potential Biomarkers for Tuberculosis: New Hope for Diagnosis?

Xia

Liu

et al. 2023

Pharmaceutics

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Tuberculosis (TB), one of the top ten causes of death globally induced by the infection of Mycobacterium tuberculosis (Mtb), remains a grave public health issue worldwide. With almost one-third of the world’s population getting infected by Mtb, between 5% and 10% of these infected individuals are predicted to develop active TB disease, which would not only result in severe tissue damage and necrosis, but also pose serious threats to human life. However, the exact molecular mechanisms underlying the pathogenesis and immunology of TB remain unclear, which significantly restricts the effective control of TB epidemics. Despite significant advances in current detection technologies and treatments for TB, there are still no appropriate solutions that are suitable for simultaneous, early, rapid, and accurate screening of TB. Various cellular events can perturb the development and progression of TB, which are always associated with several specific molecular signaling events controlled by dysregulated gene expression patterns. Long non-coding RNAs (lncRNAs), a kind of non-coding RNA (ncRNA) with a transcript of more than 200 nucleotides in length in eukaryotic cells, have been found to regulate the expression of protein-coding genes that are involved in some critical signaling events, such as inflammatory, pathological, and immunological responses. Increasing evidence has claimed that lncRNAs might directly influence the susceptibility to TB, as well as the development and progression of TB. Therefore, lncRNAs have been widely expected to serve as promising molecular biomarkers and therapeutic targets for TB. In this review, we summarized the functions of lncRNAs and their regulatory roles in the development and progression of TB. More importantly, we widely discussed the potential of lncRNAs to act as TB biomarkers, which would offer new possibilities in novel diagnostic strategy exploration and benefit the control of the TB epidemic.

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Association of lncRNA THRIL, HOTAIR genes variations and expression levels with pulmonary tuberculosis

Wang,

Li,

Zhang

et al. 2023

BMC Med Genomics

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Background Long non-coding RNA (lncRNA) has been implicated in the pathogenesis of pulmonary tuberculosis (PTB). This study aims to investigate the involvement of lncRNA THRIL and HOTAIR gene single nucleotide polymorphisms (SNPs) and their expression levels in PTB susceptibility. Methods A total of 456 PTB patients and 464 healthy controls participated in our study. we genotyped six SNPs of THRIL and HOTAIR genes using an improved multiple ligase detection reaction (iMLDR). Additionally, real-time reverse-transcriptase polymerase chain reaction was employed to detect the expression levels of THRIL and HOTAIR in peripheral blood mononuclear cells (PBMC) from 78 PTB patients and 84 healthy controls. Results No significant differences in allele and genotype frequencies were observed for THRIL rs1055472, rs11058000, and HOTAIR rs12427129, rs1899663, rs4759314, and rs7958904 polymorphisms between PTB patients and healthy controls (all P > 0.05). Moreover, genotype frequencies of all SNPs did not show any association with PTB susceptibility in the dominant–recessive model. However, the frequencies of rs7958904 CC genotype and C allele in the HOTAIR gene were significantly correlated with leukopenia in PTB patients. Furthermore, the expression levels of the HOTAIR gene were significantly elevated in PTB patients compared to controls. Conclusions Our study indicates that THRIL and HOTAIR gene SNPs might not contribute to PTB susceptibility, while the level of HOTAIR was increased in PTB patients.

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Diagnostic value of lncRNA NORAD in pulmonary tuberculosis and its regulatory role in Mycobacterium tuberculosis infection of macrophages

Cited by 4 publications

References 31 publications

Cell-specific housekeeping role of lncRNAs in COVID-19-infected and recovered patients

Cell-specific housekeeping role of lncRNAs in COVID-19-infected and recovered patients

Advances of Long Non-Coding RNAs as Potential Biomarkers for Tuberculosis: New Hope for Diagnosis?

Association of lncRNA THRIL, HOTAIR genes variations and expression levels with pulmonary tuberculosis

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