Diagnostic value and implications of vimentin expression in normal, reactive and neoplastic endocervical epithelium

Stewart, Colin J.R.; Little, Leonie

doi:10.3109/00313021003631353

Cited by 16 publications

(9 citation statements)

References 42 publications

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“…Frequently, the switch to a migratory ‘fibroblast‐like’ phenotype in epithelial cells is accompanied by the novel expression of mesenchymal markers, including vimentin. Previously, it was shown that vimentin expression in cervical squamous carcinoma occurs mainly within invasive tumour areas, 25 similar to our preliminary experience with cervical adenocarcinoma 11 . Thus the functional and immunophenotypic features of EMT may include a reduction of cell membrane E‐cadherin and beta‐catenin in conjunction with increased expression of cyclin D1 and vimentin.…”

Section: Discussionsupporting

confidence: 85%

“…At present little is known of the molecular or immunophenotypic alterations that are associated with invasion in endocervical neoplasia. However, in recent studies that were focused primarily upon ACIS, we noted that some endocervical adenocarcinomas showed a distinct pattern of vimentin and cyclin D1 expression, with immunoreactivity largely restricted to the periphery of larger tumour glands along the deep tumour margin (invasive front) or to smaller, attenuated and infiltrative glands within the stroma 11,12 . This staining pattern contrasted typically with that of the adjacent or contiguous ‘conventional’‐appearing tumour elements and was often accompanied by cytological alterations similar to those noted above in superficially invasive tumours.…”

Section: Introductionmentioning

confidence: 61%

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Correlation between invasive pattern and immunophenotypic alterations in endocervical adenocarcinoma

et al. 2011

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 61%

Correlation between invasive pattern and immunophenotypic alterations in endocervical adenocarcinoma

et al. 2011

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“…However, it has been noted that endometrial tumors showing mucinous differentiation do not necessarily demonstrate staining patterns similar to those of conventional endometrioid carcinomas and this was borne out in the current study (3,47). One possible explanation for this discrepancy, other than the focal nature of staining in many cases, is the sometimes subtle basal or lateral cell border distribution of vimentin expression in MGH, as has been noted in the normal endocervical epithelium (48,49). Conversely, vimentin expression was seen in 13/24 MGHs in agreement with the findings of Chekmareva et al (25), but not other investigators who have reported MGH to be vimentin-negative (26).…”

Section: Microglandular Lesionsmentioning

confidence: 45%

PAX2 and Cyclin D1 Expression in the Distinction Between Cervical Microglandular Hyperplasia and Endometrial Microglandular-like Carcinoma

Stewart

Crook

2015

International Journal of Gynecological Pathology

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Microglandular hyperplasia (MGH) is a common endocervical alteration that in most cases presents no diagnostic difficulty. However, MGH rarely shows atypical features that may mimic endocervical neoplasia, while conversely endometrial carcinomas can show deceptively bland MGH-like appearances. It has been suggested that immunohistochemical analysis is useful in this context, but relatively few studies have specifically investigated microglandular pattern lesions and the results have been conflicting. In this study, we have examined a series of MGH (n=24), atypical MGH (n=2), and endometrial microglandular-like carcinomas (EMC, n=8), with a panel of antibodies including PAX2, cyclin D1, p16, vimentin, and Ki67. Loss of PAX2 staining was identified only in EMC but had relatively poor sensitivity for a malignant diagnosis (3/8 cases). Seven EMCs showed p16 expression and staining was diffuse (≥50% cells) in 6 cases, whereas all conventional MGH lesions were negative. However, 1 case of atypical MGH was also p16-positive. Cyclin D1, vimentin, and Ki67 did not reliably distinguish the benign and malignant microglandular lesions because of considerable overlap in staining patterns. In summary, none of the antibodies examined proved completely sensitive and specific, but a p16-positive/PAX2-negative phenotype favored a diagnosis of EMC. Pathologists should be aware that EMC, like some other types of endometrial carcinoma, are commonly p16-positive to avoid misinterpretation as a primary endocervical neoplasm. In practice, correlation of the histologic, immunohistologic, and clinical findings is necessary for accurate interpretation of microgandular-pattern lesions, particularly in small biopsy samples.

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“…However, it has been reported that metastatic tumors from one organ to the other may show distinct profiles for bcl-2, ER, and PR in a subset of cases (27) . Although vimentin is well known to be strongly positive in endometrioid adenocarcinoma of primary uterine corpus origin, and is useful in the distinction of the latter from endocervical adenocarcinomas, its expression in primary ovarian endometrioid carcinoma has not been analyzed in detail previously (28–32) .…”

Section: Discussionmentioning

confidence: 99%

Differential Vimentin Expression in Ovarian and Uterine Corpus Endometrioid Adenocarcinomas

Desouki

Kallas

Khabele

et al. 2014

International Journal of Gynecological Pathology

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This study aimed to assess the diagnostic value of vimentin expression in differentiating endometrioid adenocarcinoma of primary uterine corpus and ovarian origin. Immunohistochemical analyses for the expression of vimentin in tumoral epithelial cells were performed on 149 endometrioid adenocarcinomas wherein the primary sites were not in question, including whole tissue sections of 27 carcinomas of uterine corpus origin (and no synchronous ovarian tumor), 7 carcinomas of ovarian origin (and no synchronous uterine corpus tumor) and a tissue microarray containing 91 primary uterine corpus and 24 primary ovarian carcinomas. We also assessed 15 cases that synchronously involved the uterine corpus and ovary, 15 cases of metastasis to organs/tissues other than uterine corpus or ovary as well as 7 lymph node metastases. Vimentin was negative in 97% (30/31) of primary ovarian carcinomas. In contrast, 82% (97/118) of primary uterine corpus carcinomas were vimentin-positive. Vimentin expression was discordant in 53% of synchronous tumors. The sensitivity and specificity of negative vimentin staining in predicting an ovarian primary were 97% and 82%, respectively whereas parallel values for positive vimentin staining in predicting a primary uterine tumor were 82% and 97% respectively. The pattern of vimentin expression in all cases was maintained in their respective regional lymph nodes and distant metastases. In conclusion, ovarian and uterine corpus endometrioid adenocarcinomas have different patterns of vimentin expression. If validated in larger and/or different data sets, these findings may have diagnostic value in distinguishing metastatic lesions from double primary tumors involving both sites.

show abstract

Diagnostic value and implications of vimentin expression in normal, reactive and neoplastic endocervical epithelium

Cited by 16 publications

References 42 publications

Correlation between invasive pattern and immunophenotypic alterations in endocervical adenocarcinoma

Correlation between invasive pattern and immunophenotypic alterations in endocervical adenocarcinoma

PAX2 and Cyclin D1 Expression in the Distinction Between Cervical Microglandular Hyperplasia and Endometrial Microglandular-like Carcinoma

Differential Vimentin Expression in Ovarian and Uterine Corpus Endometrioid Adenocarcinomas

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