Diagnostic Utility of MOC-31, HBME-1 and MOC-31mRNA in Distinguishing Between Carcinoma Cells and Reactive Mesothelial Cells in Pleural Effusions

Sun, Ying; Wu, Guang-Ping; Fang, Changqing; Liu, Shuli

doi:10.1159/000325401

Cited by 20 publications

(21 citation statements)

References 17 publications

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“…The current study included a wide variety of primary sites of metastatic adenocarcinoma, including 1 case originating in the thyroid and 1 case originating in the kidney, which to our knowledge had not been included in the studies by Morgan et al 1 or Hecht et al 19 The current series also included effusions from various body cavities, whereas the series by Sun et al focused on pleural effusions only. 20 The results of the current study found minimal and focal cytoplasmic staining in 13% of cases with reactive mesothelial cells/mesothelioma; however, we did not observe the characteristic membranous staining in these cases that is noted in adenocarcinoma. Hecht et al described positive staining for MOC-31 in scattered mesothelial cells in 9 of their 112 cases.…”

Section: -18contrasting

confidence: 88%

“…Sun et al reported a sensitivity of 70% and 100% specificity in pleural effusions. 20 The results of the current study also demonstrated a high sensitivity of MOC-31 of 89% and specificity of 100%. The current study included a wide variety of primary sites of metastatic adenocarcinoma, including 1 case originating in the thyroid and 1 case originating in the kidney, which to our knowledge had not been included in the studies by Morgan et al 1 or Hecht et al 19 The current series also included effusions from various body cavities, whereas the series by Sun et al focused on pleural effusions only.…”

Section: -18supporting

confidence: 68%

“…1,19,20 Whereas the previous studies analyzed cell block sections only, the current study reviewed not only cell block material but also alcohol-fixed cytospin preparations. Hecht et al 19 reported 100% sensitivity and 94% specificity for MOC-31 as an individual antibody in the detection of adenocarcinoma in effusion cytology specimens.…”

Section: -18mentioning

confidence: 99%

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Use of the monoclonal antibody MOC‐31 as an immunomarker for detecting metastatic adenocarcinoma in effusion cytology

Kundu

Krishnamurthy

2011

Cancer Cytopathology

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BACKGROUND:MOC‐31 is an established immunologic marker with which to detect adenocarcinomas. The objective of the current study was to evaluate the use of MOC‐31 in the diagnosis of metastatic adenocarcinoma in effusion specimens.METHODS:The authors evaluated cytologic specimens of effusions/washings in which MOC‐31 immunostaining was performed on unstained cell block sections or Papanicolaou‐stained cytospin preparations. Membranous staining with or without cytoplasmic staining was considered to be positive. The immunostaining results were correlated with the cytologic diagnoses and clinical follow‐up data.RESULTS:A total of 215 effusions and washings were identified (cell blocks in 162 cases, cytospin preparations in 53 cases, and both in 2 cases in which MOC‐31 immunostaining was performed). A total of 94 (44%) of the 215 cases were found to be positive for malignancy, including 87 metastatic adenocarcinomas. Specimens were positive for MOC‐31 in 76 (87%; 55 cell blocks and 21 cytospin preparations) of 87 cases of metastatic adenocarcinoma. Eleven cases of metastatic adenocarcinoma were found to be negative for MOC‐31 (4 cases from lung tumors, 2 from stomach tumors, 2 from colon tumors, 2 from breast tumors, and 1 from a renal tumor). Minimal and/or focal cytoplasmic staining for MOC‐31 was noted in 13% of cases of reactive mesothelial cells/mesothelioma. The sensitivity of MOC‐31 for metastatic adenocarcinoma was 89%, the specificity was 100%, the negative predictive value was 92%, and the positive predictive value was 100%.CONCLUSIONS:MOC‐31 alone was found to be highly sensitive for distinguishing reactive mesothelial cells/mesothelioma from metastatic adenocarcinoma in effusion specimens. Interpreting membranous MOC‐31 staining as positive can help prevent confusion between reactive mesothelial cells/mesothelioma and metastatic adenocarcinoma. Cancer (Cancer Cytopathol) 2011;. © 2011 American Cancer Society

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Section: -18contrasting

confidence: 88%

Section: -18supporting

confidence: 68%

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Use of the monoclonal antibody MOC‐31 as an immunomarker for detecting metastatic adenocarcinoma in effusion cytology

Kundu

Krishnamurthy

2011

Cancer Cytopathology

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“…So far, many tumor markers directed against specific cell type antigens have been used in pleural effusions to enhance the cytological diagnosis, with varying degrees of efficacy [7, 8], but the optimum panel of tumor markers still has to be reported. Moreover, the definition of earlier diagnosis of pleural effusions of patients with lung cancer by means of detecting tumor marker mRNA in occult lung cancer cells had previously been reported in our studies [9, 10]. …”

Section: Introductionmentioning

confidence: 60%

Transcription Expression and Clinical Significance of Dishevelled-3 mRNA andδ-Catenin mRNA in Pleural Effusions from Patients with Lung Cancer

Liu

Cha

et al. 2012

Clinical and Developmental Immunology

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Objective. To evaluate diagnostic utility of Dishevelled-3 (DVL-3) mRNA and δ-catenin mRNA expression in pleural effusions of patients with lung cancer. Methods. DVL-3 mRNA and δ-catenin mRNA levels were assessed by performing RT-PCR on pleural effusion specimens from patients with lung cancer (n = 75) and with lung benign disease (n = 51). Results. The expressions of DVL-3 mRNA and δ-catenin mRNA were significantly higher in malignant than in benign lung disease (P < 0.01) and were obviously higher than cytology in adenocarcinoma (P < 0.01). In single use, DVL-3 mRNA had the highest specificity (94.1%) and PPV (95.7%), whereas δ-catenin mRNA had the highest sensitivity (92.0%) and NPV (88.5%). When combinations of markers were evaluated together, DVL-3 mRNA and δ-catenin mRNA gave a high-diagnostic performance: sensitivity of 100.0%, NPV of 100.0%, and accuracy of 96.0%, respectively. Conclusion. As molecular markers of detecting pleural micrometastasis, DVL-3 mRNA and δ-catenin mRNA are helpful to diagnose the cancer cells in pleural effusions of patients with lung cancer.

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“…Therefore, there is a need for a more accurate method of detecting malignant cells. Recently, we have evaluated RT-PCR techniques for the detection of cancer cells in pleural effusions of patients with lung cancer, and have demonstrated that these techniques are more sensitive than immunocytochemistry [16,17]. RT-PCR can objectively detect even 1 cancer cell among 10 6 cells [18,19].…”

Section: Discussionmentioning

confidence: 99%

Transcription Expression and Clinical Significance of mRNA of Vascular Endothelial Growth Factor and Endostatin in Liquid-Based Preparation Specimens from Patients with Cervical Dysplasia and Carcinoma

Zhang²,

Wu³

et al. 2013

Acta Cytologica

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Objectives: The aim of this study was to assess the diagnostic usefulness of vascular endothelial growth factor (VEGF) and endostatin mRNA in cervical specimens of patients with cervical dysplasia and carcinoma. Study Design: Transcription levels of VEGF and endostatin were detected by RT-PCR in cervical liquid-based preparation specimens and compared with cytological assessments. Results: VEGF as well as endostatin mRNA expression was significantly associated with either cytological or histological diagnosis (p < 0.05). VEGF mRNA and endostatin mRNA were significantly more likely to be expressed in squamous cell carcinomas (SCC) than in cervical intraepithelial neoplasia (CIN) III (p < 0.01 and p < 0.05), and obviously also more likely to be expressed in CINII than in CINI and in CINIII than in CINII (p < 0.05). Eleven inflammation lesions gave positive results by cytology but negative results by RT-PCR for VEGF and endostatin mRNA. Twenty-four SCC lesions gave false-negative or precancerous lesion results by cytology but positive results by RT-PCR for VEGF and/or endostatin mRNA expression. Conclusion: Transcription levels of VEGF and endostatin by RT-PCR may be an adjunct to cytology screening for early detection of cervical carcinomas and may determine the progressive potentiality of individual lesions, especially in high-risk patients.

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Diagnostic Utility of MOC-31, HBME-1 and MOC-31mRNA in Distinguishing Between Carcinoma Cells and Reactive Mesothelial Cells in Pleural Effusions

Cited by 20 publications

References 17 publications

Use of the monoclonal antibody MOC‐31 as an immunomarker for detecting metastatic adenocarcinoma in effusion cytology

Use of the monoclonal antibody MOC‐31 as an immunomarker for detecting metastatic adenocarcinoma in effusion cytology

Transcription Expression and Clinical Significance of Dishevelled-3 mRNA andδ-Catenin mRNA in Pleural Effusions from Patients with Lung Cancer

Transcription Expression and Clinical Significance of mRNA of Vascular Endothelial Growth Factor and Endostatin in Liquid-Based Preparation Specimens from Patients with Cervical Dysplasia and Carcinoma

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