Diagnostic Use of CFU-E Formation from Peripheral Blood in Polycythemia Vera

Beckman, Barbara S.; Anderson, William F.; Beltran, German; Beckman, Edwin N.; Fisher, James W.

doi:10.1093/ajcp/79.4.496

Cited by 10 publications

(3 citation statements)

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“…First, while many studies find peripheral blood EECs in all patients tested [15, 16, 24, 25], other publications state that some PV patients may display EECs in bone marrow cultures but not in the periphery [26, 27]. Therefore, the absence of peripheral blood EECs in one assay does not preclude a diagnosis of PV, but necessitates either several repetitions with larger number of cells or, if possible, the investigation of bone marrow.…”

Section: Pathophysiological and Diagnostic Markersin Pvmentioning

confidence: 99%

Polycythaemia vera: Will New Markers Help Us Answer Old Questions?

Pahl

2002

Acta Haematol

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The publication of the first molecular markers for polycythaemia vera (PV) has led to a renewed interest in finding the mutations leading to the development of this myeloproliferative disorder. A substantial amount of cell biological data on the PV stem cell, however, have been available for over two decades. This review will summarise, combine and integrate both fields of investigation. Based on this analysis, two alternative models for the development of myeloproliferative disorders are proposed.

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Section: Pathophysiological and Diagnostic Markersin Pvmentioning

confidence: 99%

Polycythaemia vera: Will New Markers Help Us Answer Old Questions?

Pahl

2002

Acta Haematol

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“…Prchal and Axelrad first reported the seminal observation that in contrast to bone marrow cells from healthy individuals, bone marrow cells from PV patients form erythroid colonies in vitro without the addition of exogenous erythropoietin [42]. This erythropoietin‐independence has been observed in many different culture systems and remains one of the distinguishing characteristics of PV cells [15,43–48]. The erythropoietin‐independent colonies, which have also been named endogenous erythroid colonies (EECs), occur in 97–100% of PV patients.…”

Section: Pv Cells Are Erythropoietin‐independent and Hypersensitive Tmentioning

confidence: 99%

“…The erythropoietin‐independent colonies, which have also been named endogenous erythroid colonies (EECs), occur in 97–100% of PV patients. The EEC assay is therefore frequently used as a diagnostic tool [44,45,48,49]. In addition, Shih and Lee have successfully used the EEC assay to identify patients, who do not present with the fully developed symptoms of PV and were therefore initially diagnosed with ET [15].…”

Section: Pv Cells Are Erythropoietin‐independent and Hypersensitive Tmentioning

confidence: 99%

Towards a molecular understanding of polycythemia rubra vera

Pahl

2000

European Journal of Biochemistry

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Polycythemia rubra vera (PV) is one of four diseases collectively called the myeloproliferative disorders (MPDs). Each disorder leads to an increased production of one or several hematopoietic cell lineages. MPDs arise from acquired mutations in a pluripotent hematopoietic stem cell. However, the molecular mechanisms leading to the development of these diseases are poorly understood. This review will summarize and evaluate recent advances in our understanding of one particular MPD, PV.

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Effect of adherent cells on the regulation of BFU‐E in patients with myeloproliferative disease

et al. 1990

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The interaction between adherent cells and red cell progenitors from peripheral blood of patients with polycythemia vera (PV), essential thrombocytosis (ET), and healthy controls was studied. Various combinations of adherent and nonadherent cells were co-cultured in a semisolid system. Adherent cells from controls, when added at low concentrations, stimulated BFU-E proliferation, whereas high concentrations (40% of total cells in the culture) caused a significant decrease in the number of BFU-E colonies in 6/8 PV patients, 4/4 ET patients, and 8/12 controls. On the other hand, low and high concentrations of adherent cells from both patients with PV and ET caused a significant increase in BFU-E from either patients or controls. Moreover, adherent cells from these patients induced endogenous BFU-E proliferation (independent of erythropoietin) in nonadherent cells of 12/12 normal controls. The results show that BFU-E from patients with PV and ET are sensitive to suppression by normal adherent cells. On the other hand, adherent cells from these patients possess stimulatory activity on BFU-E from peripheral blood at all concentrations and are devoid of the inhibitory activity. This suggests a possible defect in the functioning of adherent cells in PV and ET patients which may contribute to the abnormal regulation of hematopoiesis in these disorders.

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Diagnostic Use of CFU-E Formation from Peripheral Blood in Polycythemia Vera

Cited by 10 publications

References 0 publications

Polycythaemia vera: Will New Markers Help Us Answer Old Questions?

Polycythaemia vera: Will New Markers Help Us Answer Old Questions?

Towards a molecular understanding of polycythemia rubra vera

Effect of adherent cells on the regulation of BFU‐E in patients with myeloproliferative disease

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