Diagnostic tests for tuberculous lymphadenitis: fine needle aspirations using tissue culture in mycobacteria growth indicator tube and tissue PCR

Supiyaphun, Pakpoom; Tumwasorn, Somying; Udomsantisuk, Nibondh; Keelawat, Somboon; Songsrisanga, Wilailuck; Prasurthsin, Punjapon; Sawatpanich, Ajcharaporn

doi:10.2478/abm-2010-0102

Cited by 5 publications

(4 citation statements)

References 5 publications

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“…FNA cytology also has difficulty in differentiating TB from other granulomatous or NTM diseases (Baek et al, 2000). Several researchers have performed PCR from the remainders of FNA after cytological examination, and this clinical application of PCR along with FNA cytology could reduce the necessity for open biopsy as the process of biopsy is invasive and leaves unwanted scar tissues in the neck causing aesthetic problems (Baek et al, 2000;Supiyaphun et al, 2010).…”

Section: Tuberculous Lymphadenitismentioning

confidence: 99%

Diagnosis of extrapulmonary tuberculosis by PCR

Mehta

Raj

Singh

et al. 2012

FEMS Immunol Med Microbiol

149

126

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During the last two decades, the resurgence of tuberculosis (TB) has been documented in both developed and developing nations, and much of this increase in TB burden coincided with human immunodeficiency virus (HIV) epidemics. Since then, the disease pattern has changed with a higher incidence of extrapulmonary tuberculosis (EPTB) as well as disseminated TB. EPTB cases include TB lymphadenitis, pleural TB, TB meningitis, osteoarticular TB, genitourinary TB, abdominal TB, cutaneous TB, ocular TB, TB pericarditis and breast TB, although any organ can be involved. Diagnosis of EPTB can be baffling, compelling a high index of suspicion owing to paucibacillary load in the biological specimens. A negative smear for acid-fast bacilli, lack of granulomas on histopathology and failure to culture Mycobacterium tuberculosis do not exclude the diagnosis of EPTB. Novel diagnostic modalities such as nucleic acid amplification (NAA) can be useful in varied forms of EPTB. This review is primarily focused on the diagnosis of several clinical forms of EPTB by polymerase chain reaction (PCR) using different gene targets.

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Section: Tuberculous Lymphadenitismentioning

confidence: 99%

Diagnosis of extrapulmonary tuberculosis by PCR

Mehta

Raj

Singh

et al. 2012

FEMS Immunol Med Microbiol

149

126

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“…However, the specificity is low due to the difficulty in distinguishing other granulomatous pathologies in the absence of acid‐fast bacilli that result in the same cytological features. Other conventional diagnostic tools are neither sensitive nor specific to the diagnosis of TBL . The conventional Ziehl–Neelsen (ZN) method is fast, cheap and widely used but lacks sensitivity, ranging from 20% to 43% .…”

Section: Introductionmentioning

confidence: 99%

“…The conventional Ziehl–Neelsen (ZN) method is fast, cheap and widely used but lacks sensitivity, ranging from 20% to 43% . Culture is the reference method for the detection and identification of tubercle bacilli, but may take 2–8 weeks to yield results and requires complex and specialised laboratory facilities . Although molecular methods are rapid and show promising accuracy , they are costly and not available to be routinely used in developing countries like Ethiopia, where TBL is prevalent.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic performance of fluorescent light‐emitting diode microscopy for tuberculous lymphadenitis in a high‐burden setting

Abdissa

Tadesse

Abdella

et al. 2015

Tropical Med Int Health

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The LED microscopy for tuberculous lymphadenitis had significantly higher sensitivity and shorter screening time than Ziehl-Neelsen microscopy. Use of LED microscopy among cases classified as suppurative abscess on fine-needle aspirate cytology improves evidence-based diagnosis of presumptive tuberculous lymphadenitis cases. Moreover, LED microscopy could be considered as an alternative approach in settings where fine-needle aspirate cytology is impractical.

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“…However, this method does not distinguish between EPTB and infections caused by other granulomatous diseases such as nontuberculous mycobacterial (NTM) diseases, sarcoidosis, leprosy and systemic lupus erythematosus ( Mehta et al, 2012 ). The polymerase chain reaction (PCR) technique is a useful tool for rapid diagnosis of MTB in EP samples with high sensitivity and specificity ( Supiyaphun et al, 2010 ). Among EP specimens, formalin-fixed, paraffin-embedded (FFPE) blocks can be used to study the tubercular infections.…”

Section: Introductionmentioning

confidence: 99%

Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB, and nested-rpoB PCR Cloning

et al. 2015

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Present study was aimed to examine the diagnostic utility of polymerase chain reaction (PCR) and nested PCR techniques for the detection of Mycobacterium tuberculosis (MTB) DNA in samples from patients with extra pulmonary tuberculosis (EPTB). In total 80 formalin-fixed, paraffin-embedded (FFPE) samples comprising 70 samples with definite diagnosis of EPTB and 10 samples from known non- EPTB on the basis of histopathology examination, were included in the study. PCR amplification targeting IS6110, rpoB gene and nested PCR targeting the rpoB gene were performed on the extracted DNAs from 80 FFPE samples. The strong positive samples were directly sequenced. For negative samples and those with weak band in nested-rpoB PCR, TA cloning was performed by cloning the products into the plasmid vector with subsequent sequencing. The 95% confidence intervals (CI) for the estimates of sensitivity and specificity were calculated for each method. Fourteen (20%), 34 (48.6%), and 60 (85.7%) of the 70 positive samples confirmed by histopathology, were positive by rpoB-PCR, IS6110-PCR, and nested-rpoB PCR, respectively. By performing TA cloning on samples that yielded weak (n = 8) or negative results (n = 10) in the PCR methods, we were able to improve their quality for later sequencing. All samples with weak band and 7 out of 10 negative samples, showed strong positive results after cloning. So nested-rpoB PCR cloning revealed positivity in 67 out of 70 confirmed samples (95.7%). The sensitivity of these combination methods was calculated as 95.7% in comparison with histopathology examination. The CI for sensitivity of the PCR methods were calculated as 11.39–31.27% for rpoB-PCR, 36.44–60.83% for IS6110- PCR, 75.29–92.93% for nested-rpoB PCR, and 87.98–99.11% for nested-rpoB PCR cloning. The 10 true EPTB negative samples by histopathology, were negative by all tested methods including cloning and were used to calculate the specificity of the applied methods. The CI for 100% specificity of each PCR method were calculated as 69.15–100%. Our results indicated that nested-rpoB PCR combined with TA cloning and sequencing is a preferred method for the detection of MTB DNA in EPTB samples with high sensitivity and specificity which confirm the histopathology results.

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Diagnostic tests for tuberculous lymphadenitis: fine needle aspirations using tissue culture in mycobacteria growth indicator tube and tissue PCR

Cited by 5 publications

References 5 publications

Diagnosis of extrapulmonary tuberculosis by PCR

Diagnosis of extrapulmonary tuberculosis by PCR

Diagnostic performance of fluorescent light‐emitting diode microscopy for tuberculous lymphadenitis in a high‐burden setting

Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB, and nested-rpoB PCR Cloning

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