Diagnostic significance of the human antibody response to a major cytoplasmic antigen of C. albicans

Karwowska, W.; Lisowska-Grospierre, B

doi:10.1016/0165-2478(84)90064-6

Cited by 5 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From the results that we observed, we must conclude that the molecular mass of the immunodominant antigen is 45 kDa and that our purified immunodominant antigen also has enolase activity. These findings have also been reported by others (7,12,16,22). In accordance with the results found by Mason et al (16), we also conclude from the results of the immunoblot analysis that antigenic cross-reactivity exists between our 45-kDa antigen and a commercial baker's yeast enolase.…”

Section: Discussionsupporting

confidence: 91%

Diagnostic value of anti-Candida enolase antibodies

et al. 1994

View full text Add to dashboard Cite

An immunodominant antigen with enolase enzyme activity was purified and used for the development of an assay to detect antibodies directed against this antigen in sera from patients with either invasive candidiasis or Candida colonization. The Au enzyme-linked immunosorbent assay established with the Candida enolase antigen was able to discriminate significantly between invasive candidiasis and colonization in both immunocompetent and immunodeficient groups of patients. The test had a sensitivity of 50%Y and a specificity of 86% in the immunocompetent patient group. In the immunodeficient patient group, a sensitivity of 53% and a specificity of 78% were established. Antibody levels determined by a counterimmunoelectrophoresis assay with the same set of sera resulted in a better sensitivity for sera from the immunocompetent patient group but a lower specificity, i.e., 80 and 29%o, respectively. The counterimmunoelectrophoresis assay of sera from the immunodeficient patient group was not able to discriminate significantly between invasive candidiasis and colonization. With the use of more serum from each patient, the sensitivity of the antibody detection assays increased, while the specificity was maintained. The increase, however, was not statistically significant. Combining the results of the antibody assays with antigen titers obtained by the Cand-Tec assay did not improve the predictive value with respect to invasive candidiasis, as determined by multivariance regression analysis. Furthermore, it was demonstrated by performance of Western blots (immunoblots) that sera from patients as well as a rabbit antiserum cross-reacted with the Candida enolase and baker's yeast enolase enzyme. However, by tandem crossed immunoelectrophoresis it was demonstrated that the antibodies were directed toward different epitopes of the antigen.

show abstract

Section: Discussionsupporting

confidence: 91%

Diagnostic value of anti-Candida enolase antibodies

et al. 1994

View full text Add to dashboard Cite

show abstract

“…In 1980, Jones (9) showed that cytoplasmic antigens contain large amounts of mannan and antigens from the cytoplasm of Candida species. Recently, Karwowska et al (11) reported that antibodies against the SSF which was passed through a concanavalin A Sepharose 4B column reflected the degree of C. albicans invasion. However, the sera of 16% of patients with no evidence of candidiasis were positive for antibody against the SSF in our study.…”

Section: Discussionmentioning

confidence: 99%

Enzyme-linked immunosorbent assay measurement of fluctuations in antibody titer and antigenemia in cancer patients with and without candidiasis

Fujita¹,

Matsubara²,

Matsuda³

1986

J Clin Microbiol

View full text Add to dashboard Cite

Antibody titers against purified sulfate-soluble fraction (PSSF) obtained from cytoplasmic extracts of Candida albicans were determined retrospectively over a 2-year period for 123 cancer patients by enzymelinked immunosorbent assay. Antibody against cell wall mannan (CWM) was also measured by the hemagglutination test and the production of precipitins by a serum interacting with a yeast cell homogenate by immunodiffusion. Invasive candidiasis determined by histological evidence at autopsy was present in 10 patients. Fourfold or greater rises in anti-CWM and anti-PSSF antibodies were detected for eight of the patients with invasive candidiasis at 14 to 22 days after the onset of fever. The immunodiffusion test was positive for four patients with invasive candidiasis. For patients with no evidence of candidiasis, significant rises in anti-CWM and anti-PSSF antibodies were observed at a frequency of 20 and 10%, respectively. The concentrations of serum mannan were sequentially measured by the enzyme-linked immunosorbent assay. Antigenemia (-3 ng/ml) was found in 9 of the 10 patients with invasive candidiasis and in 2 of the 4 patients with thrush, whereas the serum of 1 of the 36 patients with no evidence of candidiasis was positive for antigen. The first antigenemia antedated significant rises in antibody levels against Candida species by 6 to 23 days.

show abstract

“…Determination of HML-1 immunoglobulin subclass was performed using an enzyme-linked immunosorbent assay, rabbit antisera to mouse immunoglobulin isotypes and goat anti-rabbit IgG antisera conjugated with alkaline phosphatase (Miles Scientific, Paris, France) as described [35]. This indicated that HML-1 antibody belonged to the IgGza subclass.…”

Section: Production and Characterization Of The Hml-1 Mabmentioning

confidence: 99%

A monoclonal antibody (HML‐1) defining a novel membrane molecule present on human intestinal lymphocytes

et al. 1987

View full text Add to dashboard Cite

A monoclonal antibody, HML-1, was produced by fusion of NSI myeloma cells with spleen cells of a mouse immunized with isolated human intestinal intraepithelial lymphocytes (IEL). Immunofluorescence studies of isolated cells, as well as immunoperoxidase staining of tissue sections, indicated that HML-1 labeled all the various subsets of human intestinal IEL, approximately 40% of lamina propria T cells, 30% mesenteric lymphoblasts and some lymphocytes in other mucosae, particularly IEL. Conversely, it revealed only rare cells in all other lymphoid compartments. Analysis by polyacrylamide gel gradient electrophoresis showed that HML-1 precipitated two major noncovalently bound components of approximate mol. masses of 105 and 150 kDa from human IEL. HML-1 thus defines a novel human membrane antigen present on a subpopulation of lymphocytes preferentially associated with epithelia, and particularly with the intestinal epithelium. The characteristics of this human antigen are very similar to those of an antigen we had previously described in the rat. The possible functional role of this novel class of lymphocyte membrane antigens as well as the nature of the mechanism that triggers their expression remain to be elucidated.

show abstract

Diagnostic significance of the human antibody response to a major cytoplasmic antigen of C. albicans

Cited by 5 publications

References 6 publications

Diagnostic value of anti-Candida enolase antibodies

Diagnostic value of anti-Candida enolase antibodies

Enzyme-linked immunosorbent assay measurement of fluctuations in antibody titer and antigenemia in cancer patients with and without candidiasis

A monoclonal antibody (HML‐1) defining a novel membrane molecule present on human intestinal lymphocytes

Contact Info

Product

Resources

About