Diagnostic significance of serum lncRNA HOTAIR and its predictive value for the development of chronic complications in patients with type 2 diabetes mellitus

Abstract

Background Type 2 diabetes mellitus (T2DM) affects the social economy and quality of life, and has become a major threat to human health. This observation aimed to study the possibility of serum HOTAIR as a diagnostic index in patients with T2DM and to explore the prognostic potential of HOTAIR in the development of T2DM. Methods The expression of HOTAIR in serum of 96 patients with T2DM and 82 healthy controls was detected by the qRT-PCR technique. The related biochemical indexes of all participants were determined, such as total cholesterol (TC) and fasting blood glucose (FBG). The value of serum HOTAIR in the diagnosis of T2DM in the two groups was analyzed by the ROC curve. Moreover, the prognostic value of HOTAIR on T2DM was examined by the K-M curve and COX multivariate analysis. Results The results of the qRT-PCR analysis showed that the serum level of HOTAIR in patients with T2DM was significantly higher than that in healthy controls. ROC analysis showed that HOTAIR in serum was a diagnostic factor of T2DM. Further multivariate analysis showed that HOTAIR could be an independent biomarker in the prediction of chronic complications for T2DM patients, such as diabetic retinopathy and diabetic nephropathy. Conclusions We found the augment of HOTAIR expression was a character of T2DM. The high expression of serum HOTAIR was a potential non-invasive diagnostic marker and independent prognostic factor in patients with T2DM.

Association of Polymorphisms within HOX Transcript Antisense RNA (HOTAIR) with Type 2 Diabetes Mellitus and Laboratory Characteristics: A Preliminary Case-Control Study

“…Additionally, the circulating lncRNAs are indicative of various diseases; some of these are unique, but several are common in several diseases and as novel diagnostic and prognostic biomarkers of human diseases [ 68 ]. Many recent studies have shown that lncRNAs participate in the occurrence and development of other human diseases and diabetes and play critical regulatory roles [ 69 ]. Research articles on the significance of HOTAIR have been widely published in the literature, primarily in cancer.…”

“…HOTAIR polymorphism can be detected in diabetic patients and considered as diagnostic, prognostic, and therapeutic biomarkers. Besides, HOTAIR and fast blood glucose were independent biomarkers of T2DM, respectively [ 69 ].…”

Expressions of Serum lncRNAs in Diabetic Retinopathy – A Potential Diagnostic Tool

“…The lncRNAs used in the PCR panel are well-documented in literature. Specifically, HOTAIR is a trans-acting lncRNA that can directly interact with several epigenetic enzymes (e.g., polycomb repressive complex 2; PRC2) to regulate the expressions of multiple genes involved in various disease processes, including cancer progression, ischemic stroke, diabetes, and recently angiogenesis in DR ( 12 , 29 , 39 , 40 ). As for the lncRNA MALAT1 (also known as NEAT2 ), aberrant MALAT1 expressions have been documented in the retinas of diabetic rodents and various retinal cells cultured in high glucose environments ( 31 ).…”

Bibliometric Study of Trends in the Diabetic Nephropathy Research Space from 2016 to 2020

“…Measurements of urinary protein levels are commonly used for the diagnosis of DN in clinical practice, but other urinary and blood-based biomarkers have also been suggested to be of value for the early diagnosis and/or prognostic evaluation of DN patients [ 1 , 20 ]. These biomarkers include fibrosis-related factors, exosomes, noncoding RNAs, secreted proteins, and proinflammatory cytokines [ 21 , 22 ]. In the strategic diagram, cluster 0 was located within quadrant I, consistent with the central position and mature status of these topics, indicating that they have been active research hotspots over the past 5 years.…”