SummaryBackgroundConstipation can be diagnosed clinically using the Rome criteria. Ultrasound (US), which lacks the radiation exposure of conventional X‐ray, holds promise as a non‐invasive tool to evaluate colonic contents and constipation.AimTo examine the role of US in the assessment of constipation.MethodsWe performed a systematic search of Embase (OVID, 1984), Medline (Ovid, 1946), Cochrane Central, ClinicalTrials.gov and Australia New Zealand Clinical Trials Registry from database inception to 26 January 2024 according to PRISMA guidelines and prospectively registered with PROSPERO. All studies using US to assess constipation or colonic contents in either adults or children were included. Rectal diameter measurements were pooled in meta‐analysis. Risk of bias was assessed using the Newcastle Ottawa Scales and Joanna Briggs Institute checklists.ResultsOf 12,232 studies screened, 51 articles (6084 patients; 3422 children) describing US to assess symptoms in patients with constipation were included. Most studies used Rome criteria to diagnose constipation. Rectal diameter was associated with clinical constipation in 29 paediatric studies (3331 patients). Meta‐analysis showed the mean rectal diameter of constipated patients was significantly higher than controls (mean difference 12 mm, 95% confidence intervals (CI): 6.48, 17.93, p < 0.0001, n = 16 studies). Other features of constipation on US included posterior acoustic shadowing and echogenicity of luminal contents.ConclusionUS is an appealing imaging modality to assess luminal contents and constipation. Further well‐designed studies are required to validate US metrics that accurately identify constipation.