Diagnostic potential for miRNAs as biomarkers for pregnancy-specific diseases

Zhao, Zhen; Moley, Kelle H.; Gronowski, Ann M.

doi:10.1016/j.clinbiochem.2013.01.026

Cited by 105 publications

(108 citation statements)

References 44 publications

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“…Recently, the aberrant expression of miRNAs has been documented in different pregnancy-related conditions, such as PE, ectopic pregnancy, gestational diabetes mellitus, small for gestational age, recurrent pregnancy loss, and preterm delivery (28). These findings may be useful to improve our understanding of the underlying pathophysiology of these conditions.…”

Section: Discussionmentioning

confidence: 99%

Dysregulated circulating miRNAs in preeclampsia

et al. 2016

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Abstract. Preeclampsia (PE) is a pregnancy-related disease with potentially severe consequences with respect to foeto-maternal morbidity and mortality. However, the molecular pathogenesis of PE remains largely unknown. Recent reports have shown that microRNAs (miRNAs or miRs) may play important roles in the development of PE. Analysing the miRNAs in sera from preeclamptic women may improve our understanding of the pathophysiological mechanisms of the disease. The aim of this retrospective study was to identify whether circulating miRNAs were differentially expressed in PE patients compared with controls. Serum samples from 23 women who developed PE were compared with samples from 44 pregnant controls. Seventeen circulating miRNAs previously described in PE were chosen for evaluation of their expression by reverse transcription quantitative polymerase chain reaction (RT-qPCR). In the maternal serum, the miR-210-3p, miR-210-5p, miR-1233-3p, and miR-574-5p levels were found to be significantly higher in the PE patients than in the controls (P<0.05). Using a logistic regression model, we evaluated the discriminant power of those differentially expressed miRNAs, and the combination of miR-210-5p and miR-574-5p yielded an area under the curve of 0.7223 for discriminating PE patients from the controls. In conclusion, the fact that four circulating miRNAs (miR-210-3p, miR-210-5p, miR-1233-3p, and miR-574-5p) were differentially expressed in the sera of women who developed PE compared with controls confirms the possible pathophysiological role of miRNAs in PE.

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Section: Discussionmentioning

confidence: 99%

Dysregulated circulating miRNAs in preeclampsia

et al. 2016

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“…Молекулярно-генетические механизмы этих нарушений изучены недостаточно. В ряде исследований была отмечена роль микроРНК в патогенезе ГСД [4] и ПЭ [5].…”

Section: актуальностьunclassified

“…По данным различных авторов, число специфичных микроРНК в зрелой плаценте че-ловека колеблется от 300 до 600 [5]. В 2008 г. Chim et al сравнили уровень экспрессии 157 микроРНК в плацентарной ткани и в об-разцах венозной крови беременных [6].…”

Section: актуальностьunclassified

“…Изменения содержания раз-личных паттернов микроРНК в плацентарной ткани на разных сроках беременности рассма-триваются как один из важных регуляторных молекулярно-генетических механизмов, обес-печивающих нормальное развитие плаценты. Предполагается, что количественные измене-ния экспрессии некоторых микроРНК могут быть биомаркерами таких грозных осложне-ний беременности, как гестоз, гестационный сахарный диабет, хроническая плацентарная недостаточность и другие [5]. Так, значитель-ное снижение концентрации трех микроРНК (mir-29a, mir-222 и miR-132) были выявлены при сроке гестации 16-19 недель в крови бе-ременных, у которых в дальнейшим развился ГСД [4].…”

Section: актуальностьunclassified

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Peculiarities of placental microRNA expression in pregnancies complicated by gestational diabetes mellitus and preeclampsia

Pakin

Vashukova²,

Kapustin³

et al. 2017

Z.akus.zen.bolezn

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The aim of study was to determine feasible changes of placental miRNAs expression profiles revealed by next generation sequencing (NGS) in pregnancies with GDM complicated or not with PE. Out of 27 miRNAs, studied expression was significantly different (FDR < 0.05) only for his-miR-45a. Comparative analysis of revealed reliable differences in expression of hsa-miR-4532 (p < 0.0001, FDR = 0.0008), hsa-miR-34c-5p (p < 0.0001, FDR = 0.0083), and hsa-miR-193b-5p (p < 0.0001, FDR = 0.0139) in pregnancy complicated by PE, without of GDM. The present results suggest that GDM and PE are associated with specific alterations in the placental miRNA expression profiles. Further studies are needed to verify the role of these microRNA in molecular mechanisms underlying GDM and PE pathogenesis.

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“…MiRNAs are aberrantly expressed in many diseases, and their stability and resistance to degradation makes them useful biomarkers in cancer, autoimmune diseases, sepsis, and acute myocardial infarction among others [11]. Pregnancy-associated circulating miRNAs have been researched extensively in recent years as potential biomarkers for the diagnosis of pregnancy-associated complications, and alterations in the concentration of these miRNAs has been shown to reflect pathological states such as preeclampsia, preterm labor, and fetal growth restriction among others [12-16]. …”

Section: Introductionmentioning

confidence: 99%

MicroRNA-873 is a Potential Serum Biomarker for the Detection of Ectopic Pregnancy

Yan²,

Xu³

et al. 2017

Cell Physiol Biochem

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Background: Ectopic pregnancy (EP) refers to the implantation of the zygote outside the uterine cavity. In clinical practice, the diagnosis of EP relies on a combination of ultrasound findings and serum human chorionic gonadotrophin (hCG) measurements. However, the need for serial hCG measurements increases the risk of tubal rupture and death, underscoring the need to identify biomarkers for the early detection of EP. Methods: The serum concentrations of 21 microRNAs (miRNAs) associated with pregnancy or with known placental expression, as well as serum hCG and progesterone levels were analyzed 36 patients with viable intrauterine pregnancy (VIP), 30 patients with spontaneous abortion (SA), and 34 patients with EP using specific assay kits and reverse transcription PCR. The diagnostic performance of the different serum markers for detecting EP was analyzed by ROC curve analysis. Results: Five miRNAs were differentially expressed between the three groups, of which miR-873 and miR-223 were significantly lower in EP than in VIP and SA patients and did not change significantly according to gestational age, and miR-323 was significantly higher in EP than in VIP and SA. As a single marker, miR-873 had the highest sensitivity for detecting EP at 61.76% (at a fixed specificity of 90%). In comparison, the combination of hCG, progesterone and miR-873 had the highest sensitivity for detecting EP at 79.41% (at a fixed specificity of 90%). Conclusion: Although further validation in large-scale prospective studies is necessary, our results suggest that miR-873 could be a valuable noninvasive and stable biomarker for the early detection of EP.

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Diagnostic potential for miRNAs as biomarkers for pregnancy-specific diseases

Cited by 105 publications

References 44 publications

Dysregulated circulating miRNAs in preeclampsia

Dysregulated circulating miRNAs in preeclampsia

Peculiarities of placental microRNA expression in pregnancies complicated by gestational diabetes mellitus and preeclampsia

MicroRNA-873 is a Potential Serum Biomarker for the Detection of Ectopic Pregnancy

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