Diagnostic performance of the “MESACUP anti-Skin profile TEST”

Abstract

Background:The "MESACUP anti-Skin profile TEST" is a new, commercially available ELISA kit to detect circulating IgG autoantibodies against desmoglein 1, desmoglein 3, BP180, BP230, and type VII collagen, both simultaneously and more rapidly than previous assays. Objectives: The aim of this study was to evaluate the diagnostic accuracy of this kit for the diagnosis of pemphigus foliaceus, pemphigus vulgaris, bullous pemphigoid and epidermolysis bullosa acquisita. Materials & Methods: Dual-centre retrospective study in which 138 patients with autoimmune blistering diseases were compared to 40 controls Results: Using the MESACUP anti-Skin profile TEST, both sensitivities and specificities for desmoglein 1, desmoglein 3, BP180, BP230, and type VII collagen autoantibodies were similar to those obtained using previous, specific ELISA systems and 88% of the results were concordant without any significant difference. Conclusion: The MESACUP anti-Skin profile TEST had a similar performance to previously produced ELISA systems. The novel kit can be used for rapid diagnosis of most common autoimmune blistering diseases and is especially suitable for identifying overlapping disorders.

Serological diagnostics in the detection of IgG autoantibodies against human collagen VII in epidermolysis bullosa acquisita: a multicentre analysis

“…Thus, early diagnosis is essential for the optimal therapeutic and functional management of EBA. The detection of IgG autoantibodies directed against Col7, which play an essential pathogenic role (Table ), is central to the proper diagnosis of the different clinical EBA variants . In general, anti‐Col7 IgG serum concentrations correlate with the disease status, which is also reflected by the longitudinal analyses of three selected patients with EBA in the present study (Fig.…”

Diagnosis of Autoimmune Blistering Diseases

“…In addition, two multivariant ELISA systems compiled of the individual assays include recombinant Dsg 1 and 3, BP180 NC16A, BP230, type VII collagen, and only in one system, envoplakin, are widely available ( 75 , 76 ).…”

Diagnosis of autoimmune bullous diseases

“…They allow simultaneous testing of BP180, BP230, Dsg1, Dsg3, type VII collagen (MESACUP anti‐skin profile, MBL) and of additional envoplakin (Dermatology Profile ELISA, Euroimmun) in a single serum sample in one row of an ELISA plate. Both multivariant assays have been successfully applied in the routine diagnosis of AIBDs, and revealed high concordance with single test analyses in 88 % and 87 % of the results . In an experimental setup, the Immuno Solid‐Phase Allergen Chip (ISAC) microarray (Thermo Fischer Scientific Phadia AB, Uppsala, Sweden) has been shown to simultaneously detect IgE and IgG antibodies against BP180 NC16A, replicating corresponding ELISA values .…”

“…Vor kurzem gelang mit einem Mosaik aus vier Biochips (rekombinantes BP180 NC16A, BP230-exprimierende HEK-Zellen, NaCl-separierte Spalthaut und Affenösophagus) bei 100% der getesteten PG-Seren der Nachweis von zirkulierenden Autoantikörpern [ 36 ] . [ 105,106 ] . In einer experimentellen Anordnung konnte der Immuno Solid-Phase Allergen Chip (ISAC) Microarray (Thermo Fisher Scientifi c Phadia AB, Uppsala, Schweden) gleichzeitig IgE-und IgG-Antikörper gegen BP180 NC16A nachweisen und damit entsprechende ELISA-Werte reproduzieren [ 107 ] .…”